Background-Sustained cardiac pressure overload-induced hypertrophy and pathological remodeling frequently leads to heart failure. Casein kinase-2 interacting protein-1 (CKIP-1) has been identified to be an important regulator of cell proliferation, differentiation, and apoptosis. However, the physiological role of CKIP-1 in the heart is unknown. Methods and Results-The results of echocardiography and histology demonstrate that CKIP-1-deficient mice exhibit spontaneous cardiac hypertrophy with aging and hypersensitivity to pressure overload-induced pathological cardiac hypertrophy, as well. Transgenic mice with cardiac-specific overexpression of CKIP-1 showed resistance to cardiac hypertrophy in response to pressure overload. The results of GST pull-down and coimmunoprecipitation assays showed the interaction between CKIP-1 and histone deacetylase 4 (HDAC4), through which they synergistically inhibited transcriptional activity of myocyte-specific enhancer factor 2C. By directly interacting with the catalytic subunit of phosphatase 2A, CKIP-1 overexpression enhanced the binding of catalytic subunit of phosphatase-2A to HDAC4 and promoted HDAC4 dephosphorylation. Conclusions-CKIP-1 was found to be an inhibitor of cardiac hypertrophy by upregulating the dephosphorylation of HDAC4 through the recruitment of protein phosphatase 2A. These results demonstrated a unique function of CKIP-1, by which it suppresses cardiac hypertrophy through its capacity to regulate HDAC4 dephosphorylation and fetal cardiac genes expression. (Circulation. 2012;126:3028-3040.)Key Words: hypertrophy Ⅲ molecular biology Ⅲ cardiomyopathy Ⅲ heart failure D espite recent treatment advances, heart failure continues to impose a substantial healthcare burden. One of the major risk factors for developing heart failure is preexisting cardiac hypertrophy resulting from pathological stimuli, such as long-standing hypertension or myocardial infarction. 1,2 Among the intracellular signaling pathways involved in the regulation of cardiac hypertrophy, class II histone deacetylases (HDACs) act as signal-responsive repressors by inhibiting the activity of myocyte-specific enhancer factor 2C (MEF2C) in the nucleus. [3][4][5] Dynamic nucleocytoplasmic shuttling has been proposed as one of the most fundamental mechanisms regulating the function of class II HDACs. 4,6,7 Phosphorylation of class II HDACs at specific serine residues after hypertrophic stimulation induces its interaction with 14-3-3, through which the class II HDACs are exported to the cytosol, where they can no longer suppress target transcription factors. 4,8 -10 In the heart, nuclear export of class II HDACs directly elicits activation of myocyte enhancer factor-2 (MEF2), which is a master positive regulator of cardiac hypertrophy. Serine/threonine protein phosphatase 2A (PP2A) could interact with and dephosphorylate HDAC4, thus reinforcing its nuclear accumulation. 11,12 However, little is known about the regulation of HDAC4 dephosphorylation in response to extracellular stimuli leading to car...
This review systematically summarizes challenges and strategies relating to the functional layers of PSCs and their integration aimed at performance improvement and commercialization.
Nine cultivars of winter rapa with different cold-resistance were studied in the original growing regions and north extending regions. The results showed that the over-wintering rate of winter rapeseed reduced from 93.0%-100.0% to 40.0%-95.0%; The vegetative growth period before wilt leaf stage and the regreen stage to maturity was shortened greatly, while the duration from wilt leaf stage to regreen stage was longer than winter rapa planted in the original regions. The plant height and branch length were shortened, the 1000-grain weight and the seeds in each lique were higher at Zhangye than at Tianshui. The ultra winter-hardy cultivars and the reasonable growing date and density should be adopted in order to increase the over-winter rate of winter rapeseed in winter rapeseed extending northern regions.
Gene flow may occur between genetically modified (GM) crops and wild relatives and the fate of escaped transgenes depends on the performance of hybrids and the fitness of the transgene. Hybrids were formed by hand-crossing a GM strain of glyphosate-tolerant soybean and its non-GM counterpart with wild soybean and were assessed in this study. The hundred-seed weight of hybrids was significantly higher than that of wild soybean. However, no overall difference in plant growth was found between GM and non-GM hybrids. The results suggest that the herbicide-resistant transgene may not pose fitness costs and could persist in nature.
Background: China is generally regarded internationally as an “authoritarian” state. Traditional definitions have assigned many negative connotations surrounding the term of authoritarian. We realize that it might not be considered value-neutral in other countries. But authoritarian in the Chinese context emphasizes more on centralized decision making, collectivism, coordinating all activities of the nation, and public support, which is considered a value-neutral term. Therefore, it is adopted in this paper. We would like to clarify this. Authoritarian governance is considered an important mechanism for developing China's economy and solving social problems. The COVID-19 crisis is no exception. Most of the current research on crisis management and government crises focuses on advanced, democratic countries. However, the consequences of crisis management by authoritarian governments have not been fully appreciated. Although prior research has addressed authoritarian initiatives to manage crises in China, authoritarian interventions have rarely been theorized in public health emergencies.Methods: Based on a literature review and theoretical analysis, we use a descriptive and qualitative approach to assess public health policies and mechanisms from an authoritarian perspective in China. In light of the key events and intervention measures of China's government in response to COVID-19, the strategic practices of the Communist Party of China (CPC) to construct, embody, or set political goals through authoritarian intervention in public health crisis management are discussed.Results: China's government responded to the COVID-19 pandemic with a comprehensive authoritarian intervention, notably by establishing a top-down leadership mechanism, implementing a resolute lockdown, rapidly establishing square cabin hospitals, enhancing cooperation between different government departments, mobilizing a wide range of volunteer resources, enforcing the use of health codes, imposing mandatory quarantine on those returning from abroad, and implementing city-wide nucleic acid testing. These measures ensured that China was able to contain the outbreak quickly and reflect on the unique role of the Chinese authoritarian system in responding to public health crises.Conclusions: Our paper contributes to expanding the existing understanding of the relationship between crisis management and authoritarian system. China's response to COVID-19 exemplifies the unique strengths of authoritarian institutions in public health crisis management, which is a helpful and practical tool to further enhance the CPC's political legitimacy. As a socialist model of crisis management with Chinese characteristics, it may offer desirable experiences and lessons for other countries still ravaged by the epidemic.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
334 Leonard St
Brooklyn, NY 11211
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.