Influence of Horizontal Clinostat Rotation on Plant Proteins: 1. Effects on Ubiquitinated Polypeptides in the Stroma and Thylakoid Membranes of Vicia faba L. Chloroplasts

Wolf, D.; Schulz, Margot; Schnabl, Heide

doi:10.1016/s0176-1617(11)81739-6

Cited by 11 publications

(3 citation statements)

References 26 publications

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“…A lack of discernable changes in gross total soluble protein patterns in chronically rotated Arabidopsis stem and leaf tissue appears to be in conflict with recent reports of clinorotation effects on protein expression in Vicia faba leaves (Schulz et al, 1992;Hunte et al, 1993;Wolf et al, 1993). However, in contrast to the present study, those experiments used relatively large plants subjected to short periods of clinorotation.…”

Section: Discussioncontrasting

confidence: 99%

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Protein Expression in Arabidopsis thaliana after Chronic Clinorotation

Piastuch

Brown

1995

Journal of Plant Physiology

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SummarySoluble protein expression in Arabidopsis thaliana L. (Heynh.) leaf and stem tissue was examined after chronic clinorotation. Seeds of Arabidopsis were germinated and plants grown to maturity on horizontal or vertical slow-rotating clinostats (1 rpm) or in stationary vertical control units. Total soluble proteins and in vivo-labeled soluble proteins isolated from these plants were analyzed by two-dimensional SDS PAGE and subsequent fluorography. Visual and computer analysis of the resulting protein patterns showed no significant differences in either total protein expression or in active protein synthesis between horizontal clinorotation and vertical controls in the Arabidopsis leaf and stem tissue. These results show chronic clinorotation does not cause gross changes in protein expression in Arabidopsis.

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Section: Discussioncontrasting

confidence: 99%

“…Recent work demonstrated that membrane enzyme activities and protein turnover (Wolf et al, 1993;Hunte et al, 1993;Schulz et al, 1992) are affected by transient Clinorotation in leaves of Vicia faba. From these results, the authors suggested that Clinorotation is acting as a significant stressor of plant metabolism.…”

Section: Introductionmentioning

confidence: 99%

Protein Expression in Arabidopsis thaliana after Chronic Clinorotation

Piastuch

Brown

1995

Journal of Plant Physiology

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“…An elegant series of experiments has shown that ubiquitin is responsible for degradation of phytochrome in the light (Jabben, Shanklin, and Vierstra, 1989), but the function of this process is still in doubt. However, in plants ubiquitin has been implicated in alkaloid synthesis (Fernandez and DeLuca, 1994), petal senescence (Courtney, Rider, and Stead, 1994), pollen development (Callis and Bedinger, 1994), effects of heat shock and other stress events (Jamet et al, 1990;Rickey and Belknap, 1991;Genshik, et al, 1992;Gindin and Borachov, 1992;Almoguera, Coca, and Jordano, 1993;Cornejo et al, 1993;Wolf, Schulz, and Schnabl, 1993), as well as in lesion formation during the hypersensitive response (Becker et al, 1993).…”

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Localization of ubiquitin to differentiating vascular tissues

Stephenson

Collins

Reid

et al. 1996

American J of Botany

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The selective degradation of proteins, an essential process of any developmental program, may entail conjugation of the protein to be destroyed to the polypeptide ubiquitin. Experiments were designed to localize ubiquitin as a first step in determining whether this molecule is crucial for certain developmental processes in plant tissues and cells. Antibodies to ubiquitinated protein were detected on tissue prints of cross sections of bean petioles (Phaseolus vulgaris, Fabaceae), cotton hypocotyls (Gossypium hirsutum, Malvaceae), and Coleus stems (Coleus x hybridus, Lamiaceae). For most of the material investigated, there appears to be an accumulation of ubiquitin antibodies in vascular tissues, but not preferentially in the abscission zone of bean petioles. Vascular localization was confirmed using immunohistochemical methods on fixed and sectioned internodal tissues of Coleus. Antibodies to ubiquitin are detected in parenchyma cells of the cortex and pith, but are most concentrated in the xylem, especially secondary xylem, and in the cambial region, and in the phloem. Thus, ubiquitin accumulates in certain vascular tissues, some of which may be undergoing programmed cell death. Ubiquitin can also be detected in nondifferentiating cells, and its level is elevated in rapidly dividing cambial cells.

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Biotechnology in Space

Schnabl¹

2001

Biotechnology

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Influence of Horizontal Clinostat Rotation on Plant Proteins: 1. Effects on Ubiquitinated Polypeptides in the Stroma and Thylakoid Membranes of Vicia faba L. Chloroplasts

Cited by 11 publications

References 26 publications

Protein Expression in Arabidopsis thaliana after Chronic Clinorotation

Protein Expression in Arabidopsis thaliana after Chronic Clinorotation

Localization of ubiquitin to differentiating vascular tissues

Biotechnology in Space

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