Protein Expression in Arabidopsis thaliana after Chronic Clinorotation

Piastuch, W. C.; Brown, Christopher S.

doi:10.1016/s0176-1617(11)82063-8

Cited by 7 publications

(2 citation statements)

References 18 publications

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“…There are many different designs for clinostats, most recently reviewed by Hoson et al (1997). Each design has its problems and may not adequately simulate the space environment (Briegleb 1992; Piastuch and Brown 1995; Hilaire et al 1996). In one study by Brown et al (1996), a 2D clinostat was found to provide a good control for short, dark grown, oat coleoptiles grown in space, but not for tall plants of the same population.…”

Section: Discussionmentioning

confidence: 99%

The effect of a microgravity (space) environment on the expression of expansins from the peg and root tissues of Cucumis sativus

Link¹,

Wagner²,

Cosgrove³

2001

Physiologia Plantarum

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In young cucumber seedlings, the peg is a polar outgrowth of tissue that functions by snagging the seed coat, thereby freeing the cotyledons. The development of the peg is thought to be gravity-dependent and has become a model system for plant-gravity response. Peg development requires rapid cell expansion, a process thought to be catalyzed by alpha-expansins, and thus was a good system to identify expansins that were regulated by gravity. This study identified 7 new alpha-expansin cDNAs from cucumber seedlings (Cucumis sativus L. cv Burpee Hybrid II) and examined their expression patterns. Two alpha-expansins (CsExp3 and CsExp4) were more highly expressed in the peg and the root. Earlier reports stated that pegs tend not to form in the absence of gravity, so the expression levels were compared in the pegs of seedlings grown in space (STS-95), on a clinostat, and on earth (1 g). Pegs were observed to form at high frequency on clinostat and space-grown seedlings, yet on clinostats there was more than a 4-fold reduction in the expression of CsExp3 in the pegs of seedlings grown on clinostats vs. those grown at 1 g, while the CsExp4 gene appeared to be turned off (below detection limits). There were no detectable differences in expansin gene expression levels for the pegs of seedlings grown in space or in the orbiter environmental simulator (OES) (1 g) at NASA. The microgravity environment did not affect the expression of CsExp3 or CsExp4, and the clinostat did not simulate the microgravity environment well.

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Section: Discussionmentioning

confidence: 99%

The effect of a microgravity (space) environment on the expression of expansins from the peg and root tissues of Cucumis sativus

Link¹,

Wagner²,

Cosgrove³

2001

Physiologia Plantarum

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“…Stems may then grow horizontally, instead of upward, and roots may grow toward the stem, rather than downward under the gravity effect 4 . Several reports have elucidated the effect of clinorotation on aspects of plant physiology, such as cytoskeletal function 5 , calcium distribution 6 , the cell cycle 7 , carbohydrate metabolism 8 and protein expression 9 . The growth rates of many plant organs, such as lettuce hypocotyl, garden cress hypocotyl, and wheat coleoptile were increased under microgravity conditions 10 , 11 .…”

Section: Introductionmentioning

confidence: 99%

Gravistimulation effects on Oryza sativa amino acid profile, growth pattern and expression of OsPIN genes

Farooq

Jan

Kim

2020

Sci Rep

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Gravity is an important ecological factor regulating plant growth and developmental processes. Here we used various molecular and biochemical approaches to investigate artificial and normal gravistimulation’s effect on the early growth stages of rice (Oryza sativa L.) by changing the orientations of Petri dishes. Rate of amino acid formation, root and shoot growth, and OsPIN expression was significantly higher under gravistimulation compared with the control. Clinostat rotation positively affected plant growth and amino acid profile. However, under normal gravity, vertical-oriented seedlings showed high amino acid levels compared with clinostat, 90°-rotated, and control seedlings. Similarly, seedling growth significantly increased with 90°-rotated and vertical orientations. Artificial gravity and exogenous indole-3-acetic acid induced OsPIN1 expression in the roots, root shoot junction, and shoots of clinorotated seedlings. Phenyl acetic acid induced OsPIN1 expression in the roots and root shoot junction of clinorotated seedlings but not in the shoot. The current study suggests that OsPIN1 is differentially regulated and that it might be involved in the regulation of plant growth. Conversely, OsPIN2 and OsPIN3a are gravity sensors and highly induced in the roots and root shoot junctions of vertical and 90°-rotated seedlings and play an important role in stress conditions. Thus, on exposure to gravity, hormones, and UV-C radiation, these genes are highly regulated by jasmonic acid, 6-benzylaminopurine and gibberellic acid.

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Regeneration of plant cell protoplasts under microgravity: Investigation of protein patterns by SDS-PAGE and immunoblotting

1996

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As part of the D-2 Spacelab mission, tobacco (Nicotiana tabacum L.) protoplasts were cultured for 10 days in microgravity and successfully regenerated into microcalli, which, after further cultivation on the ground, gave rise to intact plants. Protein analysis was performed on samples taken during the initial microgravity period and compared to ground controls. Total protein content and protein patterns were monitored, as well as the cytoskeletal proteins tubulin and actin, a key enzyme of secondary metabolism, phenylalanine ammonia lyase, and the pathogenesis-related protein osmotin. None of the investigated proteins showed a gravity-dependent effect. Since relative changes due to culture age were detectable in the immunoblots as well as in the total protein pattern, an adaptation of the cells to microgravity without major modifications of their protein complement may be assumed.

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Protein Expression in Arabidopsis thaliana after Chronic Clinorotation

Cited by 7 publications

References 18 publications

The effect of a microgravity (space) environment on the expression of expansins from the peg and root tissues of Cucumis sativus

The effect of a microgravity (space) environment on the expression of expansins from the peg and root tissues of Cucumis sativus

Gravistimulation effects on Oryza sativa amino acid profile, growth pattern and expression of OsPIN genes

Regeneration of plant cell protoplasts under microgravity: Investigation of protein patterns by SDS-PAGE and immunoblotting

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