Influence of herbicide, 2,4-dichlorophenoxy acetic acid, on haemocyte DNA of in vivo treated mussel

Mičić, Milena; Bihari, Nevenka; Mlinarič-Raščan, Irena

doi:10.1016/j.jembe.2004.05.007

Cited by 14 publications

(7 citation statements)

References 40 publications

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“…As similar to the our results, the cytotoxic/cytostatic effects of some different synthetic auxins were reported in the previous studies performed on various test systems (Di Paolo et al 2001;Tuschl and Schwab 2003;Mičić et al 2004;Gonzàlez et al 2006Gonzàlez et al , 2007Gonzàlez et al , 2011.…”

Section: Discussionsupporting

confidence: 93%

In vitro genotoxicity assessment of the synthetic plant growth regulator, 1-naphthaleneacetamide

Kocaman

Güven

2015

Cytotechnology

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1-Naphthaleneacetamide (NAAm) is a synthetic plant growth regulator in the auxin family that is widely used in agriculture to promote the growth of numerous fruits, for root cuttings and as a fruit thinning agent. The potential genotoxic effects of NAAm were investigated in vitro by the chromosome aberrations (CAs), and cytokinesis-block micronucleus assays in human peripheral blood lymphocytes (PBLs) for the first time. The human PBLs were treated with 20, 40, 80, and 160 lg/mL of NAAm for 24 and 48 h. The results of this study showed that NAAm significantly induced the formation of structural CA and MN for all concentrations (20, 40, 80 and 160 lg/mL) and treatment periods (24 and 48 h) when compared with the negative and the solvent control. In addition, the higher concentrations of NAAm (80 and 160 lg/mL) caused a statistically significant increase in nuclear bud (NBUD) formation for both 24 and 48 h treatment times. With regard to the cell cycle kinetics, at all the tested concentrations, NAAm caused a statistically significant reduction in the mitotic index (MI) only for 48 h treatment period and also in the nuclear division index (NDI) for both 24 and 48 h treatment periods as compared to the control groups. The reductions in the MI and NDI occured in a concentration-dependent manner for both treatment times. In conclusion, the present results indicate that in the tested experimental conditions, NAAm was genotoxic and cytotoxic on human PBLs in vitro.

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Section: Discussionsupporting

confidence: 93%

In vitro genotoxicity assessment of the synthetic plant growth regulator, 1-naphthaleneacetamide

Kocaman

Güven

2015

Cytotechnology

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“…Moreover, pesticide exposure at 78C had no direct effect on measures of immunity, because phagocytic activity and efficiency were not affected. However, a suppression of hemocyte number was registered after 14 and 28 d. Indeed, at least one of the pesticides present in the test herbicide (2,4-D) is known to be cytotoxic and to stimulate apoptosis in hemocytes of mussels treated in vivo at concentrations between 100 and 500 mg/g, injected below the mantle [29].…”

Section: Pesticide Effects In Specimens Kept At 78cmentioning

confidence: 99%

Physiological effects of temperature and a herbicide mixture on the soft‐shell clam Mya arenaria (Mollusca, Bivalvia)

Greco

Pellerin

Capri

et al. 2010

Enviro Toxic and Chemistry

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Abstract-The aim of the current study was to investigate effects of temperature and a mixture of herbicides on the physiological status of the bivalve Mya arenaria. Bivalves acclimated to two temperatures (7 and 188C) were exposed for 28 d to 0.01 mg/L of a pesticide formulation containing dichlorophenoxyacetic acid (2,4-D), 2-(2-methyl-4-chlorophenoxy) propionic acid (mecoprop), and 3,6-dichloro-2-methoxybenzoic acid (dicamba). At days 7, 14, and 28, mortality, immune parameters (hemocyte number, phagocytic activity, and efficiency), biomarkers of oxidative stress (catalase [CAT] and superoxide dismutase [SOD] activities and malondialdehyde [MDA] content), the metabolic enzyme cytochrome C oxidase (CCO), a biomarker of pesticide exposure (acetylcholinesterase [AChE]), and the activity of an enzyme related to gametogenesis (aspartate transcarbamylase [ATCase]) were monitored in clam tissues. Gonadosomatic index (GSI), condition factor (CF), and sex were also assessed. In clams acclimated to 78C, exposure to pesticide enhanced CCO activity and CF and decreased MDA content, hemocyte number, CAT, and SOD activities. In clams kept at 188C, pesticide effects appeared minor compared with samples kept at 78C. In bivalves acclimated to 188C, CCO, SOD, and ATCase activity and MDA content were enhanced, and hemocyte number, CAT, and AchE activities and phagocytosis were suppressed. In samples exposed to pesticides, increased temperature enhanced MDA content and CCO and SOD activity and suppressed hemocyte number and CAT and AchE activity. A gradual sexual maturation was observed in both sexes through experimental time, but females had a higher sensitivity to temperature and pesticides compared to males. Increased temperature altered the ability of the sentinel species Mya arenaria to respond to pesticide exposures. Further work is needed to understand the impacts of increasing temperature on the whole St. Lawrence estuary ecosystem. Environ. Toxicol. Chem. 2011;30:132-141. # 2010 SETAC

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“…It belongs to the phenoxy herbicide family and is still one of the systemic herbicides widely used to control many types of broadleaf weeds (1). It is used to control aquatic vegetation, in pasture and rangeland applications, in cultivated agriculture, forest management, home, and garden.…”

mentioning

confidence: 99%

“…In a previous study, Benli et al (2) estimated the 96 h LC 50 for freshwater crayfish to be 32.6 mg L -1 . In addition, 2,4-D exposure has been linked with gonadal tumours in shellfish (1,(13)(14)(15)(16). Limited information about the effects of this widely used herbicide 2,4-D on the aquatic invertebrate histology and biochemical biomarkers are available in the open literature.…”

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confidence: 99%

The sublethal effects of (2,4-Dichlorophenoxy) acetic acid (2,4-D) on narrow-clawed crayfish (Astacus leptodactylus Eschscholtz, 1823)

Benlı

Şahin

Sarıkaya

et al. 2016

Archives of Industrial Hygiene and Toxicology

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2,4-D is a widely used phenoxy herbicide, potentially toxic to humans and biota. The objective of the present study was to reveal short term sublethal effects of 2,4-D on narrow-clawed freshwater crayfish (Astacus leptodactylus Eschscholtz, 1823), based on histology, total haemocyte counts, selected haemolymph parameters, and oxidative stress parameters. In the laboratory conditions crayfish specimens were exposed to 9 mg L -1 of 2,4-D for one week. Experiments were conducted under semi-static conditions in 20 L-capacity aquaria where 10 freshwater crayfish were stocked per aquarium. Exposure (experimental) and control groups were used and the experiments were repeated two times. No mortality and behavioural changes were recorded during the experiments. Total haemocyte counts decreased significantly, while haemolymph glucose levels increased (P<0.05), when compared to the control group. Haemolymph levels of calcium, chloride, sodium, potassium, magnesium, total protein, and lactate did not change. Exposure resulted with increased levels of malondialdehyde (MDA) only in hepatopancreas. However, results of gill FOX assay showed a significant decrease in oxidative stress parameters (P<0.05). MDA levels of gill and abdominal muscle tissues and FOX levels of hepatopancreas and abdominal muscle tissues did not change when compared to the control group. Significant histopathological alterations were observed both in hepatopancreas (multifocal deformations in tubule lumen) and gill tissue (melanisation of gill lamella). Exposure of crayfish even to a sublethal concentration of 2,4-D alters histopathology and lipid peroxidation due to stress. Biomarkers studied here seem to be useful for the assessment of adverse/toxic effects of pesticides on non-target, indicator aquatic organisms.KEY WORDS: haemolymph; herbicide; histology; oxidative stress; toxicity T h e f i r s t s u c c e s s f u l s e l e c t i v e h e r b i c i d e , 2,4-dichlorophenoxy acetic acid (2,4-D), was developed in 1946. It belongs to the phenoxy herbicide family and is still one of the systemic herbicides widely used to control many types of broadleaf weeds (1). It is used to control aquatic vegetation, in pasture and rangeland applications, in cultivated agriculture, forest management, home, and garden. It acts by sustaining high levels of the plant hormone auxin, which results in overstimulation of plant growth and death. In addition to this, it causes changes in the animal nervous system based on receptor interaction/interference of acetylcholine. Furthermore, it inhibits the acetyl cholinesterase (AChE) activity and increases the level of serotonin.Available data on 2,4-D toxicity on aquatic organisms mostly rely on the reports of its acute toxic effects observed in different fish species. Oruc and Uner (4) studied the combined effects of azinphosmethyl and 2,4-D on Oreochromis niloticus to clarify the mode of its action on the cellular defence system. Farah et al. (5) (8); for catfish (Clarias batrachus) by studying micronuclei and erythrocyte alt...

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Influence of herbicide, 2,4-dichlorophenoxy acetic acid, on haemocyte DNA of in vivo treated mussel

Cited by 14 publications

References 40 publications

In vitro genotoxicity assessment of the synthetic plant growth regulator, 1-naphthaleneacetamide

In vitro genotoxicity assessment of the synthetic plant growth regulator, 1-naphthaleneacetamide

Physiological effects of temperature and a herbicide mixture on the soft‐shell clam Mya arenaria (Mollusca, Bivalvia)

The sublethal effects of (2,4-Dichlorophenoxy) acetic acid (2,4-D) on narrow-clawed crayfish (Astacus leptodactylus Eschscholtz, 1823)

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