Influence of GST- and P450-based metabolic resistance to pyrethroids on blood feeding in the major African malaria vector Anopheles funestus

Nouage, Lynda; Elanga-Ndille, Emmanuel; Binyang, Achille; Tchouakui, Magellan; Atsatse, Tatiane; Ndo, Cyrille; Kekeunou, Sévilor; Wondji, Charles S.

doi:10.1371/journal.pone.0230984

Cited by 5 publications

(3 citation statements)

References 52 publications

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“…RNAi knockdown of RproCYP4M1 increased the susceptibility and mortality of its nymphs subsequently exposed to deltamethrin (Paim et al, 2021). As a salivary protein, CYP6P9a is associated with feeding success and blood meal size of A. funestus (Nouage et al, 2020). TmCYP345C4, TmCYP307A1, TmCYP4BN60, and several other genes were highly or specifically expressed in salivary gland.…”

Section: Resultsmentioning

confidence: 99%

Genome‐wide and expression‐profiling analyses of the cytochrome P450 genes in Tenebrionidea

Wang

et al. 2022

Arch Insect Biochem Physiol

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Cytochrome P450 monooxygenases (CYPs) are present in almost all areas of the tree of life. As one of the largest and most diverse superfamilies of multifunctional enzymes, they play important roles in the metabolism of xenobiotics and biosynthesis of endogenous compounds, shaping the success of insects. In this study, the CYPome (an omics term for all the CYP genes in a genome) diversification was examined in the four Tenebrionidea species through genome-wide analysis. A total of 483 CYP genes were identified, of which 103, 157, 122, and 101 were respectively deciphered from the genomes of Tebebrio molitor, Asbolus verucosus, Hycleus cichorii and Hycleus phaleratus. These CYPs were classified into four major clans (mitochondrial, CYP2, CYP3, and CYP4), and clans CYP3 and CYP4 are most diverse. Phylogenetic analysis showed that most CYPs of these Tenebrionidea beetles from each clan had a very close 1:1 orthology to each other, suggesting that they originate closely and have evolutionally conserved function. Expression analysis at different developmental stages and in various tissues showed the life stage-, gut-, salivary gland-, fat body-, Malpighian tubule-, antennae-, ovary-and testis-specific expression patterns of T. molitor CYP genes, implying their various potential roles in development, detoxification, immune response, digestion, olfaction, and reproduction.Our studies provide a platform to understand the evolution

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Section: Resultsmentioning

confidence: 99%

Genome‐wide and expression‐profiling analyses of the cytochrome P450 genes in Tenebrionidea

Wang

et al. 2022

Arch Insect Biochem Physiol

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“…Previous work on insecticide resistance markers has shown an association between the CYP6P9a gene (a marker of cytochrome P450, which mediates metabolic resistance again pyrethroid insecticides) and the feeding success and blood meal size in An. funestus [ 59 ] . These findings highlight the need for further studies to improve knowledge of the influence of multiple insecticide resistance markers harbouring on the propensity of malaria vectors to blood feed.…”

Section: Discussionmentioning

confidence: 99%

Putative pleiotropic effects of the knockdown resistance (L1014F) allele on the life-history traits of Anopheles gambiae

Medjigbodo

Djogbénou

Djihinto

et al. 2021

Malar J

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Background Existing mechanisms of insecticide resistance are known to help the survival of mosquitoes following contact with chemical compounds, even though they could negatively affect the life-history traits of resistant malaria vectors. In West Africa, the knockdown resistance mechanism kdrR (L1014F) is the most common. However, little knowledge is available on its effects on mosquito life-history traits. The fitness effects associated with this knockdown resistance allele in Anopheles gambiae sensu stricto (s.s.) were investigated in an insecticide-free laboratory environment. Methods The life-history traits of Kisumu (susceptible) and KisKdr (kdr resistant) strains of An. gambiae s.s. were compared. Larval survivorship and pupation rate were assessed as well as fecundity and fertility of adult females. Female mosquitoes of both strains were directly blood fed through artificial membrane assays and then the blood-feeding success, blood volume and adult survivorship post-blood meal were assessed. Results The An. gambiae mosquitoes carrying the kdrR allele (KisKdr) laid a reduced number of eggs. The mean number of larvae in the susceptible strain Kisumu was three-fold overall higher than that seen in the KisKdr strain with a significant difference in hatching rates (81.89% in Kisumu vs 72.89% in KisKdr). The KisKdr larvae had a significant higher survivorship than that of Kisumu. The blood-feeding success was significantly higher in the resistant mosquitoes (84%) compared to the susceptible ones (34.75%). However, the mean blood volume was 1.36 µL/mg, 1.45 µL/mg and 1.68 µL/mg in Kisumu, homozygote and heterozygote KisKdr mosquitoes, respectively. After blood-feeding, the heterozygote KisKdr mosquitoes displayed highest survivorship when compared to that of Kisumu. Conclusions The presence of the knockdown resistance allele appears to impact the life-history traits, such as fecundity, fertility, larval survivorship, and blood-feeding behaviour in An. gambiae. These data could help to guide the implementation of more reliable strategies for the control of malaria vectors.

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“…These biological processes include adult survivorship, fecundity, blood-feeding, male mating success, development of immature stages and also vector competence [11][12][13][14][15][16] . Most of these studies were generally carried out under optimal laboratory conditions and involved unrelated resistant and susceptible strains which often differ in many other genes as they originate differently.…”

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confidence: 99%

Insecticide Resistant Anopheles Gambiae Have Enhanced Longevity but Reduced biting Frequency and Reproductive Fitness

Osoro

Machani

Ochomo

et al. 2021

Preprint

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Background: Widespread insecticide resistance in African malaria vectors raises concerns over the potential to compromise malaria vector control interventions. Understanding the evolution of resistance mechanisms, and whether the selective disadvantages are large enough to be useful in resistance management or designing suitable control strategies is crucial. This study assessed whether insecticide resistance to pyrethroids has an effect on the gonotrophic cycle and reproductive potential of malaria vector Anopheles gambiae.Methods: Comparative tests were performed with a pyrethroid resistant and susceptible colonies of Anopheles gambiae colonized from the same geographical area, and the reference Kisumu strain was used as a control. Adult females aged 2 days old were given a blood meal and kept separately for individual egg laying. The number of days taken to lay eggs post blood feeding was recorded to determine the length of gonotrophic cycle. To measure adult longevity and reproduction potential, newly emerged males and females of equal numbers were aspirated into a cage and females allowed to blood feed daily. The number of eggs laid and the surviving mosquitoes were recorded daily to determine fecundity, net reproduction rate, intrinsic growth rate and adult longevity. Results: Overall, the resistant females had a significantly longer (1.8 days) gonotrophic cycle than susceptible females (F 2, 13 =9. 836, P <0.01). The proportion of resistant females that laid eggs was lower 31.30% (94/300) compared to 54% (162/300) in the susceptible colony and 65.7% (197/300) in the Kisumu strain. The mean number of eggs laid per female was significantly lower in the resistant colony (88.02 ± 20) compared to the susceptible colony (104.9 ± .28.8) and the Kisumu strain (97.6 ± 34.8). The adult longevity was significantly higher for resistant (39.7 ± 1.6 days) compared to susceptible (29.9 ± 1.7 days) and the Kisumu strain was (29.6 ± 1.1 days) (F 2,8 =45.05, P<0.0001). Resistant colony exhibited a lower fecundity (4.3 eggs/females/day) and net reproductive rate (2.6 offsprings/female/generation) compared to the susceptible colony (8.6 eggs/female/day; 4.7 off springs/female/generation respectively) and Kisumu strain (9.7 eggs/female/day; 4.1 off springs/female/generation respectively).Conclusion: The study suggests high fitness cost on reproductive parameters of pyrethroid resistant mosquitoes particularly on the duration of gonotrophic cycle, fecundity and net reproductive rate. These fitness costs are likely associated with maintaining both target site and metabolic mechanisms of resistance to pyrethroids. Despite these costs, the resistant mosquitoes had a longer longevity. The results of this study are critical to determining the extent to which insecticide resistance interacts with mosquito reproduction potential and should provide key information in the design of more targeted insecticide resistance management strategies.

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Influence of GST- and P450-based metabolic resistance to pyrethroids on blood feeding in the major African malaria vector Anopheles funestus

Cited by 5 publications

References 52 publications

Genome‐wide and expression‐profiling analyses of the cytochrome P450 genes in Tenebrionidea

Genome‐wide and expression‐profiling analyses of the cytochrome P450 genes in Tenebrionidea

Putative pleiotropic effects of the knockdown resistance (L1014F) allele on the life-history traits of Anopheles gambiae

Insecticide Resistant Anopheles Gambiae Have Enhanced Longevity but Reduced biting Frequency and Reproductive Fitness

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