Insecticide Resistant Anopheles Gambiae Have Enhanced Longevity but Reduced biting Frequency and Reproductive Fitness

Abstract

Background: Widespread insecticide resistance in African malaria vectors raises concerns over the potential to compromise malaria vector control interventions. Understanding the evolution of resistance mechanisms, and whether the selective disadvantages are large enough to be useful in resistance management or designing suitable control strategies is crucial. This study assessed whether insecticide resistance to pyrethroids has an effect on the gonotrophic cycle and reproductive potential of malaria vector Anopheles gambiae.Methods: Comparative tests were performed with a pyrethroid resistant and susceptible colonies of Anopheles gambiae colonized from the same geographical area, and the reference Kisumu strain was used as a control. Adult females aged 2 days old were given a blood meal and kept separately for individual egg laying. The number of days taken to lay eggs post blood feeding was recorded to determine the length of gonotrophic cycle. To measure adult longevity and reproduction potential, newly emerged males and females of equal numbers were aspirated into a cage and females allowed to blood feed daily. The number of eggs laid and the surviving mosquitoes were recorded daily to determine fecundity, net reproduction rate, intrinsic growth rate and adult longevity. Results: Overall, the resistant females had a significantly longer (1.8 days) gonotrophic cycle than susceptible females (F 2, 13 =9. 836, P <0.01). The proportion of resistant females that laid eggs was lower 31.30% (94/300) compared to 54% (162/300) in the susceptible colony and 65.7% (197/300) in the Kisumu strain. The mean number of eggs laid per female was significantly lower in the resistant colony (88.02 ± 20) compared to the susceptible colony (104.9 ± .28.8) and the Kisumu strain (97.6 ± 34.8). The adult longevity was significantly higher for resistant (39.7 ± 1.6 days) compared to susceptible (29.9 ± 1.7 days) and the Kisumu strain was (29.6 ± 1.1 days) (F 2,8 =45.05, P<0.0001). Resistant colony exhibited a lower fecundity (4.3 eggs/females/day) and net reproductive rate (2.6 offsprings/female/generation) compared to the susceptible colony (8.6 eggs/female/day; 4.7 off springs/female/generation respectively) and Kisumu strain (9.7 eggs/female/day; 4.1 off springs/female/generation respectively).Conclusion: The study suggests high fitness cost on reproductive parameters of pyrethroid resistant mosquitoes particularly on the duration of gonotrophic cycle, fecundity and net reproductive rate. These fitness costs are likely associated with maintaining both target site and metabolic mechanisms of resistance to pyrethroids. Despite these costs, the resistant mosquitoes had a longer longevity. The results of this study are critical to determining the extent to which insecticide resistance interacts with mosquito reproduction potential and should provide key information in the design of more targeted insecticide resistance management strategies.

House modifications using insecticide treated screening of eave and window as vector control tool: evidence from a semi-field system in Tanzania and simulated epidemiological impact

“…When these interventions are used at a large scale (protecting >80% of the community [6]) disease reduction among all community members is observed [7][8][9][10][11][12]. The widespread use of malaria control interventions also impacts other mosquitoes, including those that are of public health importance, such as those that transmit dengue infection [13] and community adherence is improved through the reduction in nuisance biting insects [14,15]. These interventions remain a cornerstone for the control of mosquito vectors.…”