Influence of Growth Regulators on Shoot Proliferation in Quercus suber L.

Romano, Anabela; Noronha, Catarina; Martins-Loução, Maria Amélia

doi:10.1093/oxfordjournals.aob.a088513

Cited by 27 publications

(21 citation statements)

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“…In hardwood trees and a few gymnosperms, responsive tissues are found at the root-shoot junction, in root or stump sprouts, sphaeroblasts, and epicormic shoots (Bonga et al 2008(Bonga et al , 2010. In vitro plants of pedunculate, sessile, cork, and red oaks were regenerated using stump sprouts or epicormic shoots from mature trees, following the above described protocols (Chalupa 1993;Vieitez et al 1985Vieitez et al , 1993Vieitez et al , 1994Favre and Juncker 1987;San-José et al 1988Romano et al 1992;Evers et al 1993;Sánchez et al 1996). These results appear to indicate the good potential for the application of micropropagation techniques in oak GD Gresshoff and Doy (1972), MS Murashige and Skoog (1962), WPM woody plant medium (Lloyd and McCown 1980) Supplements: AC activated charcoal, BA 6-benzylaminopurine, GA 3 gibberellic acid, IAA indole-3-acetic acid, IBA 3-indolebutyric acid, TDZ thidiazuron, Z zeatin Eur J Forest Res (2012) 131:519-539 523 forestry.…”

Section: In Vitro Tissue Culturementioning

confidence: 99%

Application of biotechnological tools to Quercus improvement

et al. 2011

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The genus Quercus, which belongs to the family Fagaceae, is native to the northern hemisphere and includes deciduous and evergreen species. The trees of the different species are very important from both economic and ecological perspectives. Application of new technological approaches (which span the fields of plant developmental biology, genetic transformation, conservation of elite germplasm and discovery of genes associated with complex multigenic traits) to these long-rotation hardwoods may be of interest for accelerating tree improvement programs. This review provides a summary of the advances made in the application of biotechnological tools to specific oak species. Significant progress has been made in the area of clonal propagation via organogenesis and somatic embryogenesis (SE). Standardized procedures have been developed for micropropagating the most important European (Q. robur, Q. petarea, Q. suber) and American (Q. alba, Q. bicolor, Q. rubra) oaks by axillary shoot growth. Although regenerated plantlets are grown in experimental trials, large-scale propagation of oak species has not been carried out. The induction of SE in oaks from juvenile explants is generally not problematic, although the use of explants other than zygotic embryos is much less efficient. During the last decade, enormous advances have been made in inducing SE from selected adult trees, mainly specimens of pedunculate oak (Q. robur) and cork oak (Q. suber). Advances in the understanding of the maturation and germination steps are required for better use of embryogenic process in clonal forestry. Quercus species are late-maturing and late-flowering, exhibit irregular seed set, and produce seeds that are recalcitrant to storage by conventional procedures. Vitrification-based cryopreservation techniques were used successfully in somatic embryos of pedunculate oak and cork oak, and an applied genbank of cork oak selected genotypes is now under development. The feasibility of genetic transformation of pedunculate oak and cork oak somatic embryos by means of co-culture techniques with several strains of Agrobacterium tumefaciens has also been demonstrated. To date, most research on the genomics of Quercus species has concerned population genetics. Approaches using functional genomics to examine the molecular and cellular mechanisms that control organogenesis and or somatic embryogenesis are still scarce, and efforts on the isolation and characterization of genes related to other specific traits should be intensified in the near future, as this would help improve the practical application of clonal forestry in recalcitrant species such as oaks.

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Section: In Vitro Tissue Culturementioning

confidence: 99%

Application of biotechnological tools to Quercus improvement

et al. 2011

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“…In previous studies on cork oak micropropagation, the commonly used carbon source was sucrose (Bellarosa 1981;Pardos 1981;Manzanera & Pardos 1990;Romano & Martins-Lou~o 1992;. Since other sugars have proved more efficient for woody plant propagation, independently of their in vivo requirements, the influence of different carbon sources on the shoot proliferation and in vitro rooting of Q. suber was assayed.…”

Section: Introductionmentioning

confidence: 99%

Role of carbohydrates in micropropagation of cork oak

Romano

Noronha

Martins-Loução

1995

Plant Cell Tiss Organ Cult

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The influences of carbon sources, fructose, glucose, sorbitol and sucrose on shoot proliferation and in vitro rooting of cork oak (Quercus suber L.) were compared at a wide range of concentrations (1-6%, w/v). The highest number of shoots occurred on glucose-containing medium. Nevertheless, we have chosen 3% sucrose which induced a similar rate of proliferation but favoured shoot elongation, permitting an effectively higher number of shoots during transfers. Sorbitol and autoclaved fructose did not stimulate shoot proliferation. Adventitious root formation was strongly dependent on carbohydrate supply. Sorbitol and autoclaved fructose were completely ineffectively on rooting induction. Glucose was the most effective carbon source on rooting promotion followed by sucrose and filter-sterilized fructose. The rooting response induced by fructose was dependent on the sterilizing procedure. The number of adventitious roots produced per shoot increased with increasing glucose and sucrose concentration. The content of reducing sugars in leaves of proliferation cultures and in leaves and roots of rooted plantlets was more dependent on carbon concentration than on glucose or sucrose supplement. The results presented here show that carbohydrate requirements during cork oak micropropagation depend upon the phase of culture. Sucrose (3%) and glucose (4%) were the best carbon sources respectively during proliferation and rooting phases.

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Section: Plant Materials and Surface Sterilizationmentioning

confidence: 99%

“…The types of explants that were used were the nodal and apical explants, defoliated and surface-sterilized according to Romano et al (1992). Buds were grown under controlled conditions (glasshouse), and developed shoots, 1 to 2 cm in length, were collected for in vitro culture according to Romano et al (1992). These explants were treated with 70% ethanol for 3 min and 15% Clorox (containing 5% sodium hypochlorite) with 0.2% detergent for at least 10 to 20 min for surface sterilization, and then rinsed six times with sterilized distilled water.…”

Section: Plant Materials and Surface Sterilizationmentioning

confidence: 99%

Prospects of increasing the presence of Helianthemum kahiricum Dell. pastoral North African plant by means of micropropagation

Hamza¹,

Neffati²

2014

Afr. J. Biotechnol.

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Helianthemum kahiricum has great potential in forage, in traditional medicine, for halting desert encroachment and stabilizing sand dunes through their excellent root systems development, and in the improvement of soil organic matter content. H. kahiricum is threatened with extinction because of overgrazing abuse and increasing human disturbance. For these reasons, in vitro propagation is essential for developing efficient conservation program. Several cytokinins: indole-3-acetic acid (IAA) ratios and a range of zeatin concentrations were evaluated for their effect on shoot multiplication from apical shoots and nodal segments. The type of cytokinin and the origin of the explants were the most important factors affecting shoot multiplication. The highest shoot multiplication rate was obtained from single-nodal explants on medium hormone free. Increasing zeatin concentrations promotes decreased shoot multiplication independently of explants type, although this effect tends to decrease with higher zeatin concentration. Shoot growth was higher in apical shoots and it was not stimulated by the presence of auxin. A number of experiments were conducted to identify suitable procedures for rooting of in vitro produced shoots. Although, rooting frequency rose to 98% by in vitro culture on an auxin-free medium, the survival of the plants after 6 months of acclimatization was good (90%).

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Influence of Growth Regulators on Shoot Proliferation in Quercus suber L.

Cited by 27 publications

References 0 publications

Application of biotechnological tools to Quercus improvement

Application of biotechnological tools to Quercus improvement

Role of carbohydrates in micropropagation of cork oak

Prospects of increasing the presence of Helianthemum kahiricum Dell. pastoral North African plant by means of micropropagation

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