Influence of Growth Conditions on the Production of a Bacteriocin, Pediocin AcH, by
            <i>Pediococcus acidilactici</i>
            H

Biswas, Swadesh Ranjan; Ray, P. K.; Johnson, M. C.; Ray, Bibek

doi:10.1128/aem.57.4.1265-1267.1991

Cited by 315 publications

(189 citation statements)

References 16 publications

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“…Although factors affecting the production of the peptide antibiotic, nisin, have been studied (Hurst 1981 ;Rayman & Hurst 1984) information about other bacteriocins produced by lactic acid bacteria is scarce. The effect of pH is well documented (Barefoot & Klaenhammer 1984;Joerger & Klaenhammer 1986;Ahn & Stiles 1990;Piard et al 1990;Hastings & Stiles 1991), but only a few studies are available on the effect of some media ingredients (Biswas et al 1991) or on comparisons of bacteriocin production in different media (Geis et al 1983).…”

Section: Discussionmentioning

confidence: 99%

A comparison of factors affecting the production of two bacteriocins from lactic acid bacteria

Parente

Hill

1992

Journal of Applied Bacteriology

117

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The effect of tryptone, yeast extract, Tween 80 and initial pH on the production of enterocin 1146 and lactocin D, two bacteriocins produced by lactic acid bacteria, was studied in a basal buffered medium (tryptone‐yeast extract‐tween, TYT) using factorial experiments and empirical modelling. Production of enterocin 1146 was affected by pH, yeast extract and Tween 80 and to a lesser degree, by the initial pH of the medium. On the basis of the predictions of the models developed, three TYT media (TYT10, TYT11 and TYT30) were designed to maximize bacteriocin production while minimizing the amount of peptides in the medium. Growth and bacteriocin production by Enterococcus faecium DPC 1146 (enterocin 1146), Lactococcus lactis subsp. lactis biovar diacetylactis DPC 3286 (lactocin D) and Lact. lactis subsp. cremoris LMG2130 (lactococcin A) was compared in TYT media and seven other culture media (Elliker lactic broth, M17, M17 dialysate, MRS, tryptose phosphate, tryptone yeast extract broth, yeast glucose Lemco broth). Bacteriocin production in TYT media was comparable with that in M17 and MRS, which had a higher peptide content. TYT30 allowed good production of enterocin 1146 and lactocin D while TYT11 proved acceptable for all the strains tested.

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Section: Discussionmentioning

confidence: 99%

A comparison of factors affecting the production of two bacteriocins from lactic acid bacteria

Parente

Hill

1992

Journal of Applied Bacteriology

117

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“…Culture pH alters the microbial growth profile which in turn alters bacteriocin production, as well as its adsorption and desorption to the cell membrane. 5,15,22,28,29 It is also known that pH is involved in the regulation of the processes of post-translational modification and secretion of bacteriocins. 30 This study shows that pediocin SM-1 is secreted across only a narrow pH range.…”

Section: Effect Of Medium Ph On Pediocin Productionmentioning

confidence: 99%

“…Similarly, studies in batch and chemostat cultures have shown that several bacteriocins produced by LAB, such as nisin, pediocin and plantaricin C are produced only within narrow pH ranges or at specific pH values, while others are able to be produced over wider ranges of pH values, e.g., lactococcin 972. 20,22,28,31,32 Effect of the type of the carbon source on the growth of P. pentosaceus and bacteriocin production in batch cultures Batch cultivations were carried out using glucose, sucrose, or fructose as the main source of carbon at the concentrations of 5 and 10 g/L (the pH maintained at 6.5). Glucose and sucrose supported similar growth rates (results not shown).…”

Section: Effect Of Medium Ph On Pediocin Productionmentioning

confidence: 99%

Chemostat production of pediocin SM‐1 by Pediococcus pentosaceus Mees 1934

Papagianni

Sergelidis

2015

Biotechnology Progress

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Production of the bacteriocin pediocin SM-1 by Pediococcus pentosaceus Mees 1934 was investigated in pH-controlled batch and chemostat cultures using a complex medium containing glucose, sucrose or fructose. In chemostat cultures operated at 150 rpm, 30°C, 60% dissolved oxygen tension, pH 6.5, and D = 0.148 h(-1) , the pediocin titer reached 185 AU/mL representing an increase of 32% compared with batch cultures in which glucose was used as the carbon source. Pediocin biosynthesis was markedly affected by the growth rate of the producer microorganism. For all carbon sources tested, pediocin production appeared to take place only at dilution rates lower than μmax . However, only glucose supported production at the very low dilution rate of 0.05 h(-1) indicating a direct regulation of pediocin biosynthesis by the carbon source. Glucose supported higher biomass productivity and higher pediocin titers and yields compared with the other sugars used.

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“…Inhibitory activity was studied using the agar well diffusion assay (Biswas et al 1991) using Petri dishes containing a basal layer of 1.2% agar medium. A top layer of 2 mL of soft agar (0.7% agar) containing 100 mL of the indicator strain broth was poured, and holes (5.5 mm diameter) were made using a cork borer.…”

Section: Inhibitory Activity Assaymentioning

confidence: 99%

Characterization and partial purification of a bacteriocin‐like substance produced by thermophilic Bacillus licheniformis H1 isolated from cow manure compost

Abdel-Mohsein

Sasaki

Tada

et al. 2011

Animal Science Journal

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The production and partial characterization of bacteriocin-like substances (BLSs) produced by bacteria isolated from cow manure compost were investigated. Eight BLS producers, which exhibited inhibitory activity against pathogenic bacteria, were isolated from cow manure compost at different stages of the composting process. The pile temperature ranged from 9.1 °C to 73.2 °C. The BLSs showed thermostability, but the BLS producers were not thermostable except for the H1 producer. Thermophilic Bacillus licheniformis H1 was further characterized. The culture supernatant of B. licheniformis H1 exhibited antagonistic activity against various species of Gram-positive bacteria such as Listeria monocytogenes ATCC19111 but not against Gram-negative bacteria except Pseudomonas fluorescens ATCC11251. Inactivation of bacteriocin-like activity by α-chymotrypsin, trypsin, and papain was highly significant (P<0.001). The BLS was found to be stable under a pH range from 3 to 9 and at temperatures up to 75 °C for 60 min, but it lost activity after being autoclaved at 121 °C for 15 min. The optimum production of BLS by B. licheniformis H1 was obtained at a temperature of 55 °C. Sodium dodecyl sulfate - polyacrylamide electrophoresis analysis of concentrated partially purified supernatants collected after resting the bacterial cells at 55 °C revealed a bacteriocin-like protein with a molecular mass of approximately 3.5 kDa. This study is the first report of a BLS from thermophilic B. licheniformis with an animal compost origin.

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Influence of Growth Conditions on the Production of a Bacteriocin, Pediocin AcH, by Pediococcus acidilactici H

Cited by 315 publications

References 16 publications

A comparison of factors affecting the production of two bacteriocins from lactic acid bacteria

A comparison of factors affecting the production of two bacteriocins from lactic acid bacteria

Chemostat production of pediocin SM‐1 by Pediococcus pentosaceus Mees 1934

Characterization and partial purification of a bacteriocin‐like substance produced by thermophilic Bacillus licheniformis H1 isolated from cow manure compost

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