Characterization and partial purification of a bacteriocin‐like substance produced by thermophilic Bacillus licheniformis H1 isolated from cow manure compost

Abstract

The production and partial characterization of bacteriocin-like substances (BLSs) produced by bacteria isolated from cow manure compost were investigated. Eight BLS producers, which exhibited inhibitory activity against pathogenic bacteria, were isolated from cow manure compost at different stages of the composting process. The pile temperature ranged from 9.1 °C to 73.2 °C. The BLSs showed thermostability, but the BLS producers were not thermostable except for the H1 producer. Thermophilic Bacillus licheniformis H1 was further characterized. The culture supernatant of B. licheniformis H1 exhibited antagonistic activity against various species of Gram-positive bacteria such as Listeria monocytogenes ATCC19111 but not against Gram-negative bacteria except Pseudomonas fluorescens ATCC11251. Inactivation of bacteriocin-like activity by α-chymotrypsin, trypsin, and papain was highly significant (P<0.001). The BLS was found to be stable under a pH range from 3 to 9 and at temperatures up to 75 °C for 60 min, but it lost activity after being autoclaved at 121 °C for 15 min. The optimum production of BLS by B. licheniformis H1 was obtained at a temperature of 55 °C. Sodium dodecyl sulfate - polyacrylamide electrophoresis analysis of concentrated partially purified supernatants collected after resting the bacterial cells at 55 °C revealed a bacteriocin-like protein with a molecular mass of approximately 3.5 kDa. This study is the first report of a BLS from thermophilic B. licheniformis with an animal compost origin.

Antibacterial activity of Bacillus species-derived surfactin on Brachyspira hyodysenteriae and Clostridium perfringens

“…In addition to B. subtilis , B. licheniformis also has the ability to synthesize antimicrobial substances, such as surfactin (Pecci et al 2010; Sumi et al 2015). This antimicrobial substance shows antibacterial activity against a wide range of Gram-positive bacteria, such as Listeria monocytogenes and Methicillin-resistant S. aureus , but does not cause hemolysis or inhibit the growth of Gram-negative bacteria (Dischinger et al 2009; Abdel-Mohsein et al 2011). To date, the isoforms of surfactin and antibacterial activity in B. licheniformis have rarely been studied.…”

Isolation and Partial Characterization of an Antifungal Protein Produced by Bacillus licheniformis BS-3

“…Exported bacterial substances can fulfill different functions, B. licheniformis is known to secrete a number of different substances into the extracellular medium and this ability has been used in the fermentation industry for a long time. Many of these substances have also antifungal activity, such as peptide [ 15 , 17 ], biosurfactant [ 5 , 18 ], Chitinase [ 19 ], Laccase [ 20 ]. Many bacteriocins from Gram-positive bacteria, some of which are produced by B. licheniformis strains, have been isolated from buffalo rumen and the Amazon basin [ 1 , 8 ].…”

“…The molecular weight of F2 protein, differed clearly from other antifungal agents (enzymes, peptides and proteins) produced by B. licheniformis . B. licheniformis produces various antifungal substances with molecular masses ranging from 1.4 to 20 kDa [ 1 , 5 , 9 , 10 , 11 , 15 , 17 , 21 ].…”

Effect of Fermented Products Produced by Bacillus licheniformis on the Growth Performance and Cecal Microbial Community of Broilers under Coccidial Challenge

“…Previous studies have reported that B. licheniformis can synthesize antimicrobial lipopeptides [ 33 , 34 ]. Surfactin, one of B. licheniformis -derived antimicrobial lipopeptides, exhibits antibacterial activity against a wide range of Gram-positive bacteria, such as Listeria monocytogenes and Methicillin-resistant S. aureus , but does not cause hemolysis [ 35 , 36 ]. Our previous findings have demonstrated that surfactin isolated from fermented products inhibits the growth of C. perfringens and B. hyodysenteriae [ 13 , 14 ].…”