Influence of growing conditions on morphological and chemical properties and biological effects of extracts of Arnica montana L.

Abstract

Belgrade, 2012(1,7 -2,4%).Mikroskopskim snimanjem samlevenog biljnog materijala pod različitim uveličanjima ustanovljena je jednostavna procedura identifikacije cveta arnike u odnosu na dva pretpostavljena falsifikata. Tako Since the A. montana plant specie is not native to the region of Serbia, although it occurs spontaneously in some mountain areas of neighboring countries and the Balkans in general, the aim of this study was to find a suitable technology solution of field cultivation based on the examination of induced various ecological factor effects.In this research, beside observations connected with flower heads, as the main drug, underground parts of arnica: rhizome and roots, were also studied as side products. The first phase of the experimental work included a three-year three-factorial field experiment in different modes of production, where as the factors examined: time of plantation establishment (spring and autumn planting), fertilization (manure and NPK) and methods of propagation (from seed and clonal propagation -division of the tuft). In the second phase of the experiment, characterization of the raw material was carried out in the terms of its rapid identification, qualitative evaluations based on the content of secondary metabolites and testing of biological effects of extracts obtained.Tests were performed on the field experiment conducted in 2008 at locality of Kaludjerske Bare (1008 m) on mountain Tara, in area of Nursery production department of the National Park "Tara". Since arnica its first vegetation remains in the rosette phenophase, all measurements of morphological parameters, yields and chemical Summary Doctoral Dissertation

Chemometrics-based approach in analysis of Arnicae flos

“…In this study, the content of STLs in accessions with Bulgarian provenance agreed with that reported for Arnica by Pljevljakušić, 2014 (from 4.6 mg/g to 13.9 mg/g),[ 23 ] and was intermediate between the values reported by Heldmaier, 2007 (0.64%)[ 32 ] and Clauser et al . 2012 (up to 2.31%).…”

“…[ 29 ] On the other hand, chlorogenic acid was up to 3 orders of magnitude lower than that established by Albert et al ., 2009 (4.4–6.7 mg/g)[ 12 ] and Pljevljakušić, 2012 (1.9–6.57 mg/g). [ 23 ] The content of caffeic acid was in agreement with the literature. [ 15 29 ] The difference could be due to the methods of sample preparation and quantification used as well as the number and variety of phenolic acids included in the amounts stated.…”

“…The obtained results revealed that the dominant phenolic derivatives are chlorogenic acid, isoquercitrin, and astragalin, which was in agreement with the literature. [ 23 ] The highest total level of studied flavonoids recorded in the sample from the Botanical Garden, Turku University (E) was in the same order of magnitude as that given by Bomme, 1999 (7.00 mg/g),[ 24 ] Ganzera et al ., 2008 (0.6–1.7%),[ 15 ] Dall’Acqua et al ., 2011 (0.70–1.42%). [ 25 ] On the other hand, other authors reported significantly higher values (13.18–23.45 mg/g;[ 11 ] 10.4–24.4 mg/g;[ 26 ] 9.6–24.4 mg/g;[ 27 ] 13.18–23.45 mg/g[ 28 ]).…”

“…[ 29 ] In addition, the authors provided evidence for a higher quercetin content (1.881 mg/g) as compared to our results. The amount of isoquercitrin determined in this study was lower than the levels found by Albert et al ., 2009 (9.7–11.7 mg/g)[ 12 ] and Pljevljakušić, 2012 (7.8–13.9 mg/g),[ 23 ] while the amount of astragalin was within the range of reported values (2.2–3.7 mg/g),[ 12 ] except for the Bulgarian accessions A and I.…”

Arnica Montana L. Supercritical Extraction Optimization for Antibiotic and Anticancer Activity

“…The genus has more than 30 different species, and they differ significantly in the content of medicinal components, with A. montana being the species used most widely for its medicinal value ( Kowalski et al, 2015 ; Kriplani et al, 2017 ) and the flower heads being the part of the plant containing the highest levels of biologically active compounds ( Galambosi, 2004 ; Ganzera et al, 2008 ; Spitaler et al, 2008 ). The antimicrobial activity of the plant extracts has been studied extensively, as they have been shown to possess antimicrobial activity against a wide range of microorganisms ( Brantner and Grein, 1994 ; Koo et al, 2000 ; Klaas et al, 2002 ; Iauk et al, 2003 ; Pljevljakušić, 2013 ; Bulfon et al, 2014 ; Kryvtsova, 2020 ). The anticancer activity of various components of A. montana has been investigated thoroughly, particularly sesquiterpene lactones, the main active compound of the plant ( Lee et al, 1972 ; Lyß et al, 1998 ; Willuhn, 1998 ; Douglas et al, 2004 ; Huang P.-R. et al, 2005 ; Ghantous et al, 2010 ; Chaturvedi, 2011 ; Lim et al, 2012 ; Chadwick et al, 2013 ; Jakobs et al, 2016 ; Drogosz and Janecka, 2019 ; Kriplani and Guarve, 2020 ).…”