Influence of green tea on surface charge density and phospholipids composition of erythrocytes membrane in ethanol intoxicated rats

Szachowicz-Petelska, Barbara; Dobrzyńska, Izabela; Skrzydlewska, Elżbieta; Figaszewski, Zbigniew A.

doi:10.1007/s10565-005-1783-9

Cited by 11 publications

(1 citation statement)

References 38 publications

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“…An increase in the amount of specific phospholipids results in the appearance of additional functional groups, both positively and negatively charged, at the membrane surface. Changes in membrane structure caused by the modification of protein structure lead to yield higher negative electric charge at high pH values and lower positive electric charges at low pH values (Szachowicz-Petelska et al 2005, 2008; Dobrzyńska et al 2008). Variations in the number and kind of functional groups result in changes in C TA and C TB and, in turn, in their association constant values (Table 2).…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Blackcurrant on Liver Cell Membrane of Rats Intoxicated with Ethanol

et al. 2012

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Chronic ethanol intoxication oxidative stress participates in the development of many diseases. Nutrition and the interaction of food nutrients with ethanol metabolism may modulate alcohol toxicity. One such compound is blackcurrant, which also has antioxidant abilities. We investigated the effect of blackcurrant as an antioxidant on the composition and electrical charge of liver cell membranes in ethanol-intoxicated rats. Qualitative and quantitative phospholipid composition and the presence of integral membrane proteins were determined by high-performance liquid chromatography. Electrophoresis was used to determine the surface charge density of the rat liver cell membranes. Ethanol intoxication is characterized by changes in cell metabolism that alter the structure and function of cell membrane components. Ethanol increased phospholipid levels and altered the level of integral proteins as determined by decreased phenylalanine, cysteine, and lysine. Ethanol significantly enhanced changes in the surface charge density of the liver cell membranes. Administration of blackcurrant to rats intoxicated with ethanol significantly protected lipids and proteins against oxidative modifications. It is possible that the beneficial effect of blackcurrant is connected with its abilities to scavenge free radicals and to chelate metal ions.

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Section: Discussionmentioning

confidence: 99%

Protective Effect of Blackcurrant on Liver Cell Membrane of Rats Intoxicated with Ethanol

et al. 2012

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Influence of green tea on erythrocytes antioxidant status of different age rats intoxicated with ethanol

Łuczaj

Zapora

Skrzydlewska

2009

Phytotherapy Research

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The aim of this paper is to evaluate the effect of green tea on the erythrocyte antioxidant system of ethanol-intoxicated rats, as well as its efficacy in the prevention of lipid peroxidation. Rats (2, 12 and 24 months old) were fed on a control or an ethanol Lieber-DeCarli diet with and without green tea (7 g/L) for 5 weeks. Examination included the activity of antioxidant enzymes and the level of both non-enzymatic antioxidants and lipid peroxidation marker in rat erythrocytes. It was shown that ageing was accompanied by changes in the antioxidant enzymes activity - increase in the SOD and CAT activity and decrease in GSSG-R and GSH-Px activity, as well as in the level of non-enzymatic antioxidants - GSH, vitamin A and vitamin E. The increase in the level of lipid peroxidation marker - MDA - was also observed. Green tea consumption partially prevented lipid peroxidation process, especially in erythrocytes of 2- and 12-month-old rats. It was proved that ethanol administration caused a statistically significant decrease in the activity/level of the examined antioxidants in all age groups (the most significant in the case of 24-month-old rats) of rats, as well as an increase in the MDA level. However, ingestion of green tea by ethanol-intoxicated rats partially prevented the decrease in activity/level of all examined antioxidant parameters, as well as protected lipids against peroxidation in all age groups of rats. Obtained results confirm the beneficial effect of green tea on erythrocyte antioxidant abilities.

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Parameters characterizing acid–base equilibria between cell membrane and solution and their application to monitoring the effect of various factors on the membrane

Dobrzyńska

Skrzydlewska

Figaszewski

2006

Bioelectrochemistry

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Influence of green tea on surface charge density and phospholipids composition of erythrocytes membrane in ethanol intoxicated rats

Cited by 11 publications

References 38 publications

Protective Effect of Blackcurrant on Liver Cell Membrane of Rats Intoxicated with Ethanol

Protective Effect of Blackcurrant on Liver Cell Membrane of Rats Intoxicated with Ethanol

Influence of green tea on erythrocytes antioxidant status of different age rats intoxicated with ethanol

Parameters characterizing acid–base equilibria between cell membrane and solution and their application to monitoring the effect of various factors on the membrane

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