Influence of green tea on erythrocytes antioxidant status of different age rats intoxicated with ethanol

Abstract

The aim of this paper is to evaluate the effect of green tea on the erythrocyte antioxidant system of ethanol-intoxicated rats, as well as its efficacy in the prevention of lipid peroxidation. Rats (2, 12 and 24 months old) were fed on a control or an ethanol Lieber-DeCarli diet with and without green tea (7 g/L) for 5 weeks. Examination included the activity of antioxidant enzymes and the level of both non-enzymatic antioxidants and lipid peroxidation marker in rat erythrocytes. It was shown that ageing was accompanied by changes in the antioxidant enzymes activity - increase in the SOD and CAT activity and decrease in GSSG-R and GSH-Px activity, as well as in the level of non-enzymatic antioxidants - GSH, vitamin A and vitamin E. The increase in the level of lipid peroxidation marker - MDA - was also observed. Green tea consumption partially prevented lipid peroxidation process, especially in erythrocytes of 2- and 12-month-old rats. It was proved that ethanol administration caused a statistically significant decrease in the activity/level of the examined antioxidants in all age groups (the most significant in the case of 24-month-old rats) of rats, as well as an increase in the MDA level. However, ingestion of green tea by ethanol-intoxicated rats partially prevented the decrease in activity/level of all examined antioxidant parameters, as well as protected lipids against peroxidation in all age groups of rats. Obtained results confirm the beneficial effect of green tea on erythrocyte antioxidant abilities.

Green tea polyphenols and their potential role in health and disease

“…EGCG is known for its anti-inflammatory, hypoglycemic, hypocholesterlemic, anticancer, antihypertensive, antioxidant, antiviral, and pancreatic anticancer activities (Yousaf et al 2014). Tea extract has been reported to show an inhibitory effect on blood coagulation, prevent the onset of certain type of cardiovascular diseases [atherosclerosis], dissolve fibrinogen, inhibit platelet aggregation, (Liatsos et al 2010) lower endothelin level (Unger 2010), activate GSH-Px, protect LDL oxidation (Wojciech et al 2010), and improve glucose tolerance in diabetes (Zuo et al 2014).…”

Morphological alterations in the jejunal mucosa of aged rats and the possible protective role of green tea

“…By the administration of the green tea extract to old rats, these parameters recovered to normal values of young rats. This effect can be attributed to the antioxidative activity of green tea, since its administration prevented decreases in liver glutathione (GSH) concentrations induced by ethanol administration [44]. A previous study showed that green tea extract has the capacity to scavenge free radicals with significant increase in the activity of antioxidant enzymes such as glutathione S-transferases (GST), superoxide dismutase (SOD) and GPX [45].…”

Oxidative stress and antioxidant status in intensely exercising horses administered nutraceutical extracts

“…Retinol, also known as vitamin A, has been seen to have antioxidant properties. In a study in which healthy rats consumed a liquid diet with 7 g green tea extract/l diet for 5 weeks, vitamin A levels were significantly increased in rats aged 2 and 12 months (Wojciech et al . 2010).…”

“…Retinol, also known as vitamin A, has been seen to have antioxidant properties. In a study in which healthy rats consumed a liquid diet with 7 g green tea extract/l diet for 5 weeks, vitamin A levels were significantly increased in rats aged 2 and 12 months (Wojciech et al 2010). Based on the previous study, administering tea extracts increases retinol levels; however, further research will need to be conducted in horses to see if other antioxidant levels could be increased with multiple doses of tea extract.…”