Influence of glutathione chemical effectors in the response of maize to arsenic exposure

Requejo, Raquel; Tena, Marı́a Teresa

doi:10.1016/j.jplph.2012.01.016

Cited by 26 publications

(12 citation statements)

References 29 publications

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“…This may be related to GSH biosynthesis. As previously reported, an increase in GSH caused an increase in nonprotein thiol content, which promoted heavy metal tolerance in maize (Requejo and Tena, 2012). Phytochelatin content might also be affected by stable GSH levels because they are synthesized from GSH.…”

Section: Primer Namesupporting

confidence: 56%

Regulation of boron toxicity responses via glutathione-dependent detoxification pathways at biochemical and molecular levels in Arabidopsisthaliana

Kayıhan¹,

Kayıhan²,

Çiftçi³

2019

Turk J Bot

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The fine-tuned regulation of the Halliwell-Asada cycle (ascorbate-glutathione pathway) in Arabidopsis thaliana under boron (B) toxicity was shown in our previous report. In this study, we investigated the expression levels of some members of the glutathione S-transferase (GST) superfamily, such as phi (GSTF2, GSTF6, GSTF7, and GSTF8), tau (GSTU19), and zeta (GSTZ1) classes in Arabidopsis thaliana that were exposed to 1 mM boric acid (1B) and 3 mM boric acid (3B). Additionally, the expression levels of genes for glutathione (GSH) and phytochelatin biosynthesis as well as miR169 and miR156 were evaluated in Arabidopsis thaliana exposed to 1B and 3B. Moreover, changes in the levels of total GST activity; GSH; and total, protein-bound, and nonprotein thiols were spectrophotometrically determined. GSH levels and nonprotein thiol content did not change significantly following both B-toxicity conditions. Expression levels of GSH1 and GSH2 stayed stable under 1B toxicity; however, GSH1 expression increased significantly under 3B conditions in Arabidopsis thaliana. The expression levels of four genes from phi class members of GST were not dramatically changed under B-toxicity conditions. However, the transcript levels of miR169, ATGSTU19, and ATGSTZ1 were significantly increased after 1B and 3B exposure. These GST genes may have a role in the dramatic increase of total GST activity under toxic B. To the best of our knowledge, this is the first report displaying an integrative view of high-B-induced regulation of GSH-dependent enzymatic machinery at different biological organization levels in Arabidopsis thaliana.

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Section: Primer Namesupporting

confidence: 56%

Regulation of boron toxicity responses via glutathione-dependent detoxification pathways at biochemical and molecular levels in Arabidopsisthaliana

Kayıhan¹,

Kayıhan²,

Çiftçi³

2019

Turk J Bot

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“…It has been reported that safeners could increase the conjugation of xenobiotic compounds with GSH through elevating the level of GSH in plant (Requejo, Tena, 2012). Therefore, GSH content in plants treated with safener was an important index to access the protective ability of safener.…”

mentioning

confidence: 99%

The safener effect of chiral derivatives of 3-dichloroacetyl oxazolidine against haloxyfop-P-methyl-induced toxicity in maize

Fei

Cao

et al. 2016

Zemdirbyste-Agriculture

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Herbicide safener, a diverse group of chemicals, is an important tool used to protect plants from herbicidal injury. With the aim of decreasing drift injury of haloxyfop-P-methyl to sensitive plants, the protective effect of four safeners (R-28725, 3-dichloroacetyl oxazolidine and its two optical isomers) was evaluated. Physiological and biochemical tests were conducted under laboratory conditions in Northeast Agricultural University, China, by using seed treatment with safener and soil treatment with haloxyfop-P-methyl, respectively. The maize seeds treated with these safeners were safe from haloxyfop-P-methyl treatment. A positive correlation between growth level and endogenous glutathione (GSH) content, glutathione S-transferases (GST) activity was observed in this research. Enhancement of GSH content, GST activity and affinity of GST to 1-chloro-2,4-dinitrobenzene (CDNB) in maize treated by R-28725 was maximum. However, the detoxification of herbicide was not accompanied by the increase of acetyl-CoA-carboxylase (ACC) activity in maize.

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“…On cadmium or copper exposure A. thaliana plants responded by increasing the transcription of glutathione synthetase and glutathione reductase ( GR ) genes which were involved in GSH synthesis and reduction respectively (Queval et al ., 2009). Further, the involvement of GSH was reported in combating metal toxicity against arsenic stress in maize (Requejo and Tena, 2012), chromium toxicity in rice (Zeng et al ., 2012; Qiu et al ., 2013) and cadmium stress in Pinus and A. thaliana (Schützendübel et al ., 2001; Jobe et al ., 2012). Cross-talk of GSH with zinc and Fe homeostasis was also reported (Shanmugam et al ., 2012).…”

Section: Introductionmentioning

confidence: 99%

Glutathione regulates transcriptional activation of iron transporters via S-nitrosylation of bHLH factors to modulate subcellular iron homeostasis

Shee

Ghosh²,

Khan³

et al. 2021

Preprint

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Glutathione (GSH) is a ubiquitous molecule known to regulate various physiological and developmental phenomena in plants. Recently, its involvement in regulating iron (Fe) deficiency response was established in Arabidopsis. However, the role of GSH in modulating subcellular Fe homeostasis remained elusive. In this study, we dissected the role of GSH in regulating Fe homeostasis in Arabidopsis shoots under Fe limited conditions. The two GSH depleted mutants, cad2-1 and pad2-1 displayed increased sensitivity to Fe deficiency with smaller rosette diameter and higher chlorosis level compared with the Col-0 plants. Interestingly, the expression of the vacuolar Fe exporters, AtNRAMP3 and AtNRAMP4, chloroplast Fe importer, AtPIC1, along with AtFer1 and AtIRT1 were significantly down-regulated in these mutants. The expression of these genes were up-regulated in response to exogenous GSH treatment while treatment with BSO, a GSH inhibitor, down-regulated their expression. Moreover, the mutants accumulated higher Fe content in the vacuole and lower in the chloroplast compared with Col-0 under Fe limited condition suggesting a role of GSH in modulating subcellular Fe homeostasis. This regulation was, further, found to involve a GSNO-dependent pathway. Promoter analysis revealed that GSH induced the transcription of these genes presumably via S-nitrosylation of different Fe responsive bHLH transcription factors.

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Influence of glutathione chemical effectors in the response of maize to arsenic exposure

Cited by 26 publications

References 29 publications

Regulation of boron toxicity responses via glutathione-dependent detoxification pathways at biochemical and molecular levels in Arabidopsisthaliana

Regulation of boron toxicity responses via glutathione-dependent detoxification pathways at biochemical and molecular levels in Arabidopsisthaliana

The safener effect of chiral derivatives of 3-dichloroacetyl oxazolidine against haloxyfop-P-methyl-induced toxicity in maize

Glutathione regulates transcriptional activation of iron transporters via S-nitrosylation of bHLH factors to modulate subcellular iron homeostasis

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