Influence of Glucose Concentration on the Membrane Stability of Human Erythrocytes

Abstract

The action of glucose as an osmolyte in relation to blood cells is not well-characterized in the literature. This study aimed to study the influence of glucose concentration on the stability of red blood cells. The stability of erythrocytes was evaluated by the half-transition point obtained from the curves of lysis induced by glucose in the absence of salt or by increase in medium hypotonicity in the absence and the presence of different concentrations of glucose. In the presence of 0.9 g/dl NaCl, there was no hemolysis with increasing concentration of glucose from 0 to 10 g/dl. In the absence of NaCl, the dependence of hemolysis with the 0-10 g/dl glucose was described by a decreasing sigmoid, with fully lysed and fully protected cells being encountered in the presence of 0-2 and 4-10 g/dl glucose, respectively. The possible origin of such stabilization effect is discussed with base of what is known about osmostabilization of biological complexes and about the influence of glucose on the rheological properties of erythrocytes.

Relationship between serum cholesterol and indices of erythrocytes and platelets in the US population

“…Also, the ABO blood group type, recently shown in a genome-wide association study to be associated with LDL-C ( 47 ), was not determined in NHANES. Two additional variables that we did not analyze, but that have important effects upon erythrocyte membrane stability, are albumin concentration ( 48 ) and glucose concentration ( 49 ). Strengths of our analysis include the large size of our study population and our replication of results in a separate cohort.…”

Influence of age on the correlations of hematological and biochemical variables with the stability of erythrocyte membrane in relation to sodium dodecyl sulfate

“…1,2 The maintenance of the fluidity and functionality of a membrane depends on several factors, which include the relative content of phospholipids and cholesterol, the concentration of ions 3 and the osmolarity of the medium. 4 In erythrocytes, the membrane fluidity contributes to the deformability required for the passage of these cells through small diameter capillaries as well as for the exchange of gases between hemoglobin (Hb) and the external tissues. However, during their lifetime, the red cells undergo various chemical and mechanical stresses, resulting in loss of membrane area and deformability.…”

Cytotoxicity of N-dodecanoyl-L-homoserine lactone and 5-N-dodecyl resorcinol to human granulocytes and monocytes: a comparative study

“…In a separate experiment, the action of C 12 -HSL and C 12 -AR on erythrocyte membrane stability was investigated [17]. The 50-µl aliquots of treated and control samples were added to tubes containing 5 ml of 0.40-0.85% NaCl solutions, gently mixed, and incubated at 37°C for 30 min before being centrifuged at 1500 × g for 15 min.…”