Influence of fucoidans and their derivatives on antitumor and phagocytic activity of human blood leucocytes

Anisimova, Natalia; Ustyuzhanina, Nadezhda E.; Доненко, Ф. В.; Bilan, Maria I.; Ушакова, Н. А.; Усов, А. И.; Nifantiev, Nikolay E.; Kiselevskiy, M. V.

doi:10.1134/s0006297915070111

Cited by 18 publications

(17 citation statements)

References 20 publications

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“…Intact animals were characterized as a control. Used active concentrations were selected on the basis of our previous data [ 18 , 26 ]. The results of the investigation of hematological parameters in various groups of mice are presented in Table 1 and Figure 2 .…”

Section: Resultsmentioning

confidence: 99%

“…They are in demand for the accompanying treatment of cancer patients who suffer from hematological toxicity during chemo and/or radiation therapy. Availability of parent natural fucoidans [ 18 , 20 , 21 ] and fucosylated chondroitin sulfates [ 12 , 13 , 14 , 15 , 16 , 17 ] of very different structure as well as the possibility of chemical synthesis of oligosaccharides which represent fucoidans [ 22 , 25 , 26 , 27 ] and fucosylated chondroitin sulfates [ 23 , 24 ] fragments as well as the methods for their conformational analysis [ 27 , 28 , 29 , 30 , 31 ] permits systematic structure-activity study, to assess the pharmacophore fragments in the structures of sulfated polysaccharides and to undertake the next steps—the search for target cellular receptors and rational design of their druggable inhibitors. These studies are in progress and will be reported in due course.…”

Section: Discussionmentioning

confidence: 99%

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Fucoidan and Fucosylated Chondroitin Sulfate Stimulate Hematopoiesis in Cyclophosphamide-Induced Mice

et al. 2017

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Application of cytostatics in cancer patients’ chemotherapy results in a number of side effects, including the inhibition of various parts of hematopoiesis. Two sulfated polysaccharides, fucoidan from the seaweed Chordaria flagelliformis (PS-Fuc) and fucosylated chondroitin sulfate from the sea cucumber Massinium magnum (PS-FCS), were studied as stimulators of hematopoiesis after cyclophosphamide immunosuppression in mice. Recombinant granulocyte colony-stimulating factor (r G-CSF) was applied as a reference. Both tested polysaccharides PS-Fuc and PS-FCS have a similar activity to r G-CSF, causing pronounced neutropoiesis stimulation in animals with myelosuppression induced by cyclophosphamide (CPh). Moreover, these compounds are also capable to enhance thrombopoiesis and erythropoiesis. It should be noted that PS-FCS demonstrated a greater activity than r G-CSF. The results indicate the perspective of further studies of PS-Fuc and PS-FCS, since these compounds can be considered as potentially promising stimulators of hematopoiesis. Such drugs are in demand for the accompanying treatment of cancer patients who suffer from hematological toxicity during chemo and/or radiation therapy.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fucoidan and Fucosylated Chondroitin Sulfate Stimulate Hematopoiesis in Cyclophosphamide-Induced Mice

et al. 2017

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“…The authors [121] studied the immunotropic activity of fucoidans and their derivatives differing on Mm and structure from different algae (poly-and oligosaccharides, including HMW PSs from Chordaria flagelliformis, Saccharina latissima, LMW PS from P. plantaginea and completely sulfated LMW L-fucose octasaccharide) and established that the greatest activity in vitro was shown by HMW fucoidan from algae C. flagelliformis. Its main chain is built up of (1→3)-linked α-L-fucopyranose residues and side chains include α-D-glucuronic acid and α-L-fucopyranose residues.…”

Section: Structural-functional Relationships Problem Of Standardizatmentioning

confidence: 99%

The Sulfated Polysaccharides of Brown Algae and Products of Their Enzymatic Transformation as Potential Vaccine Adjuvants

Кузнецова

Persiyanova²,

Ermakova

et al. 2018

Natural Product Communications

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The review is devoted to critical analysis of literature data, deal with effects and mechanisms of action of sulfated polysaccharides (PSs)-fucoidans from brown algae and products of their enzymatic transformation as potential adjuvants for enhancement of anti-infective and antitumor immune response. Numerous experimental data indicate that sulfated PSs demonstrate properties of vaccine adjuvants. Application perspectiveness of fucoidans as vaccine adjuvants is defined by their high biocompatibility, low-toxicity, safety and good tolerance by macroorganism, and also mechanisms of their immunomodulatory action. In particular, fucoidans are agonists of receptors of innate immunity and strong inducers of cellular and humoral immune response. At presenting the data of structural-functional interrelations, attention focused to the defining role of degree of sulfation, uronic acids and polyphenols contents, and also molecular mass in actions of fucoidans to innate and adaptive immunity cells. Insufficiency of literary data on studying of correlation of structure-physicochemical characteristics with adjuvanticities of the sulfated PSs, and also the problem of standardization of their active fractions are noted. Special attention is paid to the analysis of immunomodulatory and adjuvant activity of fucoidan oligosaccharides. Presented here results of experimental trial indicate that, despite the difficulties due to preparation of highly purified structurally characterized fractions and complex structure of fucoidans, these substances can be used as safe and effective adjuvants in vaccines against various pathogens including viruses, and also in antitumor vaccines.

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“…The mechanisms are still unknown. However, fucoidan can directly induce cytotoxicity and apoptosis of cancer cells (14) probably explained by an additional study suggesting implication of macrophages and blood leukocytes in relation to fucoidan (15).…”

mentioning

confidence: 99%

Fucoidans Stimulate Immune Reaction and Suppress Cancer Growth

Vĕtvická

Větvičková

2017

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Abstract. Background Fucoidan is a complex sulfated polysaccharide consisting of sulfated fucose residues and found mostly in brown marine algae, echinoderms (1) and seagrasses (2). Fucoidans exhibit numerous biological activities found both in in vitro and in vivo experiments. Fucoidan extracts have been found to ameliorate acute colitis (3), have anticancer activity (4) mediate via natural killer (NK) cells (5) and affect inflammation, vascular physiology, as well as oxidative stress (6, 7). Some effects differ based on the type of extract, as documented by the differential effects of high and low molecular weight fucoidans on the severity of arthritis in mice. The low molecular weight samples reduced arthritis via suppression of Th1-mediated immunity, whereas high molecular weight samples enhanced the inflammatory activation of macrophages (8). Subsequent experiments revealed that high molecular weight fucoidans stimulated spleen cells, whereas low molecular weight samples had little activity or were toxic (9). The reasons for these differences are still unknown. Using a fish model, however, dietary fucoidan was found to influence blood chemistry and constituents, antioxidation and innate immunity (10). In human models, fucoidan delayed apoptosis and induced proinflammatory cytokines, probably via activation of the PI3K/AKT pathway (11). As fucoidan can function as an adjuvant and stimulate antibody response (12), it has been used to improve vaccine efficacy (13).Cancer development is the most studied effect of fucoidan. The mechanisms are still unknown. However, fucoidan can directly induce cytotoxicity and apoptosis of cancer cells (14) probably explained by an additional study suggesting implication of macrophages and blood leukocytes in relation to fucoidan (15).In our study, we not only directly compared the biological activities of individual fucoidan samples, but also compared purified samples with commercially available component mixtures. Thus, our study answers the question not only whether fucoidans influence immune reactions, but also if the popular idea of combining biologically active molecules together improves their effectiveness. Materials and MethodsMaterials. Individual samples were purchased from the manufacturers or distributors as shown in Table I. RPMI 1640 medium, sodium citrate, 4-(2-hydroxyethyl)-1-piperazine-ethanol-sulfonic acid (HEPES), antibiotics, Wright stain, Freund's adjuvant, ovalbumin, Concanavalin A (Con A) and lipopolysaccharide (LPS) were purchased from Sigma (St. Louis, MO, USA). Fetal calf serum (FCS) was from Hyclone Laboratories (Logan, UT, USA).Cells. J774A.1 mouse macrophages and YAC cells were obtained from the ATCC (Manassas, VA, USA). The BALB/c mouse-derived mammary tumor cell line Ptac64 was generously provided by Dr. Wei-Zen Wei of the Michigan Cancer Foundation, Wayne State University, Detroit, MI, USA. The cell lines were maintained in RPMI 1640 medium containing HEPES buffer supplemented with 10% heat-inactivated FCS, 100 U/ml penicillin and 100 μg/ml stre...

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Influence of fucoidans and their derivatives on antitumor and phagocytic activity of human blood leucocytes

Cited by 18 publications

References 20 publications

Fucoidan and Fucosylated Chondroitin Sulfate Stimulate Hematopoiesis in Cyclophosphamide-Induced Mice

Fucoidan and Fucosylated Chondroitin Sulfate Stimulate Hematopoiesis in Cyclophosphamide-Induced Mice

The Sulfated Polysaccharides of Brown Algae and Products of Their Enzymatic Transformation as Potential Vaccine Adjuvants

Fucoidans Stimulate Immune Reaction and Suppress Cancer Growth

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