Influence of Freeze-Drying and Irradiation on Mechanical Properties of Human Cancellous Bone: Application to Impaction Bone Grafting

Cornu, Olivier

doi:10.5772/32811

Cited by 8 publications

(5 citation statements)

References 119 publications

(83 reference statements)

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“…Althoug gamma irradiation is commonly used in bone banks for sterilization, it causes radiolysis of water molecules, releasing free radicals ( Nguyen et al, 2007 ) that damage collagen ( Seto, Gatt, and Dunn, 2008 ; Harrell et al, 2018 ). Previous studies on bones showed a negative synergic effect on mechanical resistance when irradiation was added to freeze-dried samples ( Cornu et al, 2000 ; Cornu et al, 2011 ), attributed to a significant reduction in enzymatic crosslinks density ( Cornu, 2012 ). This is consistent with our results revealing a more pronounced alteration of crosslinks post-irradiation.…”

Section: Discussionmentioning

confidence: 99%

Collagen molecular organization preservation in human fascia lata and periosteum after tissue engineering

Vettese,

Manon,

Chretien

et al. 2024

Front. Bioeng. Biotechnol.

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Large bone defect regeneration remains a major challenge for orthopedic surgeons. Tissue engineering approaches are therefore emerging in order to overcome this limitation. However, these processes can alter some of essential native tissue properties such as intermolecular crosslinks of collagen triple helices, which are known for their essential role in tissue structure and function. We assessed the persistence of extracellular matrix (ECM) properties in human fascia lata (HFL) and periosteum (HP) after tissue engineering processes such as decellularization and sterilization. Harvested from cadaveric donors (N = 3), samples from each HFL and HP were decellularized following five different chemical protocols with and without detergents (D1-D4 and D5, respectively). D1 to D4 consisted of different combinations of Triton, Sodium dodecyl sulfate and Deoxyribonuclease, while D5 is routinely used in the institutional tissue bank. Decellularized HFL tissues were further gamma-irradiated (minimum 25 kGy) in order to study the impact of sterilization on the ECM. Polarized light microscopy (PLM) was used to estimate the thickness and density of collagen fibers. Tissue hydration and content of hydroxyproline, enzymatic crosslinks, and non-enzymatic crosslinks (pentosidine) were semi-quantified with Raman spectroscopy. ELISA was also used to analyze the maintenance of the decorin (DCN), an important small leucine rich proteoglycan for fibrillogenesis. Among the decellularization protocols, detergent-free treatments tended to further disorganize HFL samples, as more thin fibers (+53.7%) and less thick ones (−32.6%) were recorded, as well as less collagen enzymatic crosslinks (−25.2%, p = 0.19) and a significant decrease of DCN (p = 0.036). GAG content was significantly reduced in both tissue types after all decellularization protocols. On the other hand, HP samples were more sensitive to the D1 detergent-based treatments, with more disrupted collagen organization and greater, though not significant loss of enzymatic crosslinks (−37.4%, p = 0.137). Irradiation of D5 HFL samples, led to a further and significant loss in the content of enzymatic crosslinks (−29.4%, p = 0.037) than what was observed with the decellularization process. Overall, the results suggest that the decellularization processes did not significantly alter the matrix. However, the addition of a gamma-irradiation is deleterious to the collagen structural integrity of the tissue.

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Section: Discussionmentioning

confidence: 99%

Collagen molecular organization preservation in human fascia lata and periosteum after tissue engineering

Vettese,

Manon,

Chretien

et al. 2024

Front. Bioeng. Biotechnol.

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“…Most xenografts are produced by defatting, decellularizing, and deproteinizing cancellous bone, which are necessary processes for the elimination of antigenicity, the mineralized bone matrix alone being the substance in clinical use ( Meloni et al, 2017 ). Thus, the toughness of xenograft is lowered compared to natural bone even though the strength can be mostly retained ( Cornu, 2012 ). Moreover, due to the process of sterilization and storage allografts and xenografts have significantly decreased osteogenic and osteoinductive function compared to living autografts ( Pape et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCs

Zhu

Blahnová

Perale

et al. 2020

Front. Cell Dev. Biol.

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Bone defect is a noteworthy health problem and is the second most transplanted tissue after blood. Numerous bone grafts are designed and applied in clinics. Limitations, however, from different aspects still exist, including limited supply, mechanical strength, and bioactivity. In this study, two biomimetic peptides (P2 and P6) are incorporated into a composite bioactive xeno hybrid bone graft named SmartBonePep®, with the aim to increase the bioactivity of the bone graft. The results, which include cytotoxicity, proliferation rate, confocal microscopy, gene expression, and protein qualification, successfully prove that the SmartBonePep® has multi-modal biological effects on human mesenchymal stem cells from bone marrow. The effective physical entrapment of P6 into a composite xeno-hybrid bone graft, withstanding manufacturing processes including exposure to strong organic solvents and ethylene oxide sterilization, increases the osteogenic potential of the stem cells as well as cell attachment and proliferation. P2 and P6 both show a strong biological potential and may be future candidates for enhancing the clinical performance of bone grafts.

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“…A wide variety of bone grafts are used in bone grafting procedures . However, several limitations of the current bone grafts were observed such as donor site morbidity, the possibility of disease transmission and the exhaustive processing procedures which negatively influence the structural integrity and osteoregenerative properties of different bone grafts . These many drawbacks led to an ongoing search for alternative products that can be used for bone grafting procedures.…”

Section: Introductionmentioning

confidence: 99%

Egyptian propolis compared to nanohydroxyapatite graft in the treatment of Class II furcation defects in dogs

Zohery

Meshri

Madi

et al. 2018

Journal of Periodontology

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Influence of Freeze-Drying and Irradiation on Mechanical Properties of Human Cancellous Bone: Application to Impaction Bone Grafting

Cited by 8 publications

References 119 publications

Collagen molecular organization preservation in human fascia lata and periosteum after tissue engineering

Collagen molecular organization preservation in human fascia lata and periosteum after tissue engineering

Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCs

Egyptian propolis compared to nanohydroxyapatite graft in the treatment of Class II furcation defects in dogs

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