Influence of exogenous phytase supplementation on phytate degradation, plasma inositol, alkaline phosphatase, and glucose concentrations of broilers at 28 days of age

Abstract

Inositol is the final product of phytate degradation, which has the potential to serve as an indicator of phytase efficacy. An experiment was conducted to evaluate effects of supplementing broiler diets with phytase on phytate degradation and plasma inositol concentrations at 28 d of age. Twenty-four Ross × Ross 708 male chicks were placed in battery cages (4 birds per cage) from 1 to 21 d of age and individually from 22 to 28 d of age. At 27 d of age, a catheter was placed in the brachial vein of broilers to avoid repeated puncture of the vein during blood collection. At 28 d of age, broilers received 1 of 3 experimental diets formulated to contain 0, 400, or 1,200 phytase units ( FTU )/kg, respectively, in diet 1, 2, and 3. Blood was collected 1 h before feeding experimental diets and from 20 to 240 min after feeding experimental diets at 20-min intervals with a final blood collection at 480 min to determine plasma inositol concentrations. Inositol phosphate ( IP ) ester degradation was determined in gizzard contents and ileal digesta. Broilers provided the 1,200 FTU/kg phytase diet had 60% less ( P < 0.01) IP6 concentration in gizzard content (1,264 vs. 4,176 nmol/g) and ileal digesta (13,472 vs. 33,244 nmol/g) than birds fed the 400 FTU/kg diet. Adding phytase at 1,200 FTU/kg increased ( P < 0.01) inositol concentrations in gizzard content and ileal digesta of broilers by 2.5 (2,703 vs. 1,071 nmol/g) and 3.5 (16,485 vs. 4,667 nmol/g) fold, respectively, compared with adding 400 FTU/kg. Plasma inositol concentration of broilers was not different ( P = 0.94) among the dietary treatments at each collection time. Inositol liberation in the digesta of broilers fed diets with 1,200 FTU/kg phytase did not translate to increased plasma inositol concentrations, which warrants further investigation.

High Dietary Organic Iron Supplementation Decreases Growth Performance and Induces Oxidative Stress in Broilers

“…Serum enzymes, such as ALP, ALT, and AST, serve as biomarkers of liver function [38]. ALP is mainly produced in the liver, kidney, and bones, and it increases inorganic phosphate levels and activates collagen fibers in the bone matrix; thus, ALP contributes to bone mineralization, as well as the functions of the liver and kidney [39,40]. In the current study, serum ALP activity decreased in broilers supplemented with a high dose of Fe-LG, which was consistent with the results reported by Yamasaki et al [41], who reported that high Fe exposure may inhibit ALP activity in osteoblastic cells.…”

Effect of Phytase Level and Form on Broiler Performance, Tibia Characteristics, and Residual Fecal Phytate Phosphorus in Broilers from 1 to 21 Days of Age

“…Martins et al [ 53 ] observed a reduction in residual fecal phytate P of broilers fed phytase-supplemented diets (750 FTU/kg) compared to broilers fed diets with no phytase addition. Kriseldi et al [ 54 ] reported similar results when evaluating two doses of phytase (400 and 1200 FTU/kg) on phytate concentration in the ileal digesta of 28-day-old birds. The authors reported that both concentrations of phytase effectively increased phytate degradation and this led to increased inositol liberation.…”

The Transport and Uptake of Resveratrol Mediated via Glucose Transporter 1 and Its Antioxidant Effect in Caco-2 Cells

“…In humans and animals, the gastrointestinal tract digests food and absorbs nutrients [ 1 ]. After oral administration, many Chinese herbal extracts and Chinese herbal monomers will be absorbed by the small intestine after passing through the stomach and large intestine [ 2 , 3 ]. However, intestinal absorption and bioavailability are also affected by a variety of factors (uptake, absorption time, and apparent permeation coefficient, Papp) [ 4 , 5 ].…”