Influence of exogenous fibrolytic enzymes onin vitroandin saccodegradation of forages for ruminants

Abstract

An in vitro assay was carried out to evaluate the effects of exogenous fibrolytic enzymes (1, 2, 3 and 4 g/kg DM) powder preparation containing xylanase and cellulase from Aspergillus niger and Trichoderma viride on DM, NDF and ADF degradation of alfalfa hay, corn silage, corn stover, elephant grass, Guinea grass and oat straw. Kinetics data of in vitro degradations were analyzed. The potentially degradable fraction and degradation rate of NDF and ADF of alfalfa increased quadratically (P<0.05) as the inclusion level of enzyme increased up to 3 g. The others forages were not affected by the enzyme. An in sacco trail was performed using four Holstein steers fitted with ruminal cannulas to evaluate the effects of the exogenous fibrolytic enzymes (3 g/kg DM) on DM, NDF and ADF degradation of alfalfa hay and corn stover. Kinetics data were also analyzed. The potentially degradable fraction degradation of NDF (62.0 vs 65.7%) and ADF (52.8 vs 56.9%), of alfalfa hay were increased (P<0.05) by the exogenous fibrolytic enzymes, but no differences were found for corn stover. These results suggest that the enzymes increased in vitro and in sacco fibre degradation only for alfalfa hay

Ruminal degradability of high moisture triticale (X. Triticosecale Wittmack) silage with chemical and biological additives

“…This effect may be related to the greater digestibility of fiber caused by action of enzymes present in inoculant. This improvement has already been proven in other studies (Alvarez et al, 2009;Gallardo et al, 2010).…”

Sustainable anaerobic rumen methane and carbon dioxide productions from prickly pear cactus flour by organic acid salts addition

“…It seems that rumen modulator like OAS delays the adaptation of rumen microbes to the substrate, in contrast to reports where other rumen enhancers such as S. cerevisiae shortens lag time (Rodriguez et al, 2015) or exogenous fibrolytic enzymes have no effect on lag time (Elghandour et al, 2015a). This variability in results, among others, may be due to factors such as the species and physiological status of rumen fluid donor, characteristics of the substrate used, composition and quantity of rumen modulator applied and application methods (Gallardo et al, 2010). Substrate degradation or fermentation rate has been reported to be directly proportional to GP (Dhanoa et al, 3.…”

Effect of exogenous fibrolytic enzymes supplementation on milk production and nutrient utilization in Murrah buffaloes

“…Many studies have reported increased digestion of dry matter (DM) and fiber measured in situ, in vitro (Elwakeel et al 2007;Bandla et al 2008;Gallardo et al 2010), as well as in vivo (Kung et al 2000;Alvarez et al 2009). Recent studies have also shown that adding EFE to ruminant diets increases the milk production Shojaeian and Thakur 2007b;Miachieo and Thakur 2007) and average daily gain (McAllister et al 1999;Cruywagen and Goosen 2004).…”