Effect of exogenous fibrolytic enzymes supplementation on milk production and nutrient utilization in Murrah buffaloes

Abstract

The present study was conducted to evaluate the effect of commercial exogenous fibrolytic enzyme (EFE) mixture added at 1.5 and 3.0 g (cellulase 4,000 microM glucose/g/h + xylanase 7,990 microM xylose/g/min; 50:50 w/w) per kilogram of dry matter (DM) of feed on nutrient digestibility, milk production, milk composition, and some blood constituents in lactating Murrah buffaloes. Eighteen buffaloes were allotted to three dietary treatments, on the basis of milk yield (8.48, 8.52, and 8.53 kg/day) and days in lactation (68.5, 80.33, and 82.00) for 90 days. The buffaloes were fed a total mixed ration (TMR) comprising of 45% chaffed wheat straw, 15% chopped green maize, and 40% concentrate on DM basis (control group), the same TMR plus EFE at 1.5 g/kg DM (T-1 group) and the same TMR plus EFE at 3.0 g/kg DM (T-2 group) supplemented through the concentrate mixture. There was no effect of fortifying EFE mixture on DM intake and crude protein intake (grams per day) whereas total digestible nutrients intake (kilogram per day) was higher by (P < 0.05) 12.53% in T-1 group over that of control, and there was no significant difference between T-2 and control groups. The average daily milk yield and 4% fat-corrected milk (FCM) yield was higher (P < 0.05) by 12.99% and 15.17% in T-1 group as over that of control, and there was no difference between T-2 and control groups. There was no (P > 0.05) difference in blood glucose and blood urea nitrogen concentration in different experimental groups. It is concluded that supplementation of cellulase and xylanase mixture at 1.5 g/kg of DM of TMR containing wheat straw (45%), green maize (15%), and concentrate (40%) on DM basis significantly increased (P < 0.05) the average daily milk yield and FCM yield in Murrah buffaloes due to improved dietary fiber digestion.

Effect of Feeding Xylanase and Cellulase Treated Oat Silage on Nutrient Digestibility, Growth Performance and Blood Metabolites of Nili Ravi Buffalo Calves

“…The reason may be a high metabolic rate of utilization of glucose and homeostatic mechanism of animal body does not allow appreciable change in glucose level. The blood glucose and urea levels recorded in the present study are in agreement with the results reported by Shekhar et al (2010) and Varlyakov et al (2010) in buffaloes. There was no difference in blood urea nitrogen level in control and treatment groups.…”

“…There was no difference in blood urea nitrogen level in control and treatment groups. Similarly, non-significant differences in blood glucose and urea levels were reported by Shekhar et al (2010) and Varlyakov et al (2010) in buffaloes. Buffalo calves were in positive nitrogen balance across all diets.…”

Nitrogen and Phosphorus Utilisation and Excretion in Dairy Buffalo Intensive Breeding

“…The apparent N digestibility for non-lactating and lactating buffaloes combined was around 67%. Similar values have been reported in previous studies performed in buffalo species (Shekhar et al, 2010;Saxena et al, 2010). Apparent N digestibility was greater in buffaloes at mid-and late-lactation than those in early-lactation.…”

Understanding the structural and functional properties of carbohydrate esterases with a special focus on hemicellulose deacetylating acetyl xylan esterases

“…Earlier studies have proved that supplementation of exogenous fibrolytic enzymes to animal feedstock has significantly improved the digestion of fibres (Beauchemin et al 2003). Studies have also reported that addition of xylanases, AcXE and cellulases to the animal feed stock has significantly increased the average daily milk yield among the goats and Murray buffaloes, respectively (Bala et al 2009; Shekhar et al 2010). …”