Influence of Estimated Training Status on Anti and Pro-Oxidant Activity, Nitrite Concentration, and Blood Pressure in Middle-Aged and Older Women

Abstract

The purpose of this study was to compare the association between anti and pro-oxidant activity, nitrite concentration, and blood pressure (BP) in middle-aged and older women with different levels of estimated training status (TS). The sample consisted of 155 females (50–84 years) who were submitted to a physical examination to evaluate estimated TS through the “Functional Fitness Battery Test,” BP measurements, and plasma blood samples to evaluate pro-oxidant and antioxidant activity and nitrite concentrations. Participants were separated by age into a middle-aged group (<65 years) and an older (≥65 years) group and then subdivided in each group according to TS. Blood biochemistry was similar between groups. On the other hand, protein oxidation was lower in participants with higher TS, independent of age. Older females with higher TS presented higher nitrite concentrations, lower lipoperoxidation, and lower values of BP compared with those with lower TS. Lower GPx activity was observed in participants with higher TS compared with middle-aged with lower TS. Thus, our results suggest that good levels of TS may be associated with lower oxidative stress and higher nitrite concentration and may contribute to maintain normal or reduced blood pressure values.

Dynamic Resistance Training Improves Cardiac Autonomic Modulation and Oxidative Stress Parameters in Chronic Stroke Survivors: A Randomized Controlled Trial

“…Thiobarbituric acid reactive substances (TBARS), carbonyls, NADPH oxidase, hydrogen peroxide (H 2 O 2 ), superoxide dismutase (SOD), and plasma nitrite analyses were conducted in accordance with Jacomini et al [27].…”

Effect of Functional Fitness on Plasma Oxidation Level in Elders: Reduction of the Plasma Oxidants and Improvement of the Antioxidant Barrier

“…It has been observed that regular physical exercise is essential for healthy aging and beneficial for cardiovascular system [7] and cognitive function [8]. With regard to oxidative stress, physical activity is able to regulate both proand anti-oxidant activities by preventing the exacerbation of free radical release related to older age.…”

Identification of Aortic Proteins Involved in Arterial Stiffness in Spontaneously Hypertensive Rats Treated With Perindopril:A Proteomic Approach

“…After the functional parameter measurements, all rats were deeply anesthetized by an overload of xylazine hydrochloride and ketamine hydrochloride (Anasedan ® , 20 mg/kg and Dopalen ® , 160 mg/kg, i.v., respectively, Ceva Sante Animale, Paulínea, SP, Brazil) and euthanized by decapitation. Blood samples were collected in heparinized vacutainers immediately after the euthanasia and centrifuged at 4,000 rpm for 5 min for analysis of nitrite concentration as previously published ( Jacomini et al, 2017 ). In summary, proteins were quantified using automated biochemistry equipment (model A-15, Biosystems S/A, Barcelona, Spain) to normalize the calculation of nitrite concentration.…”