Influence of enrichment media and application of a PCR based method to detect Salmonella in poultry industry products and clinical samples

Schrank, Irene Silveira; Morés, M. A. Z.; Costa, José Luiz A da; Frazzon, Ana Paula Guedes; Soncini, Ricardo Alfredo; Schrank, Augusto; Vainstein, Marilene Henning; Silva, S.C

doi:10.1016/s0378-1135(01)00350-9

Cited by 53 publications

(39 citation statements)

References 19 publications

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“…H-antigens can be expressed in one of two phases: phase 1 H-antigens are serovar specific while phase 2 antigens are not. However K-antigens are produced by serovars that are Since Salmonella is closely related to both human and animal health, more rapid and sensitive methods for the identification of this bacterium are required (Schrank et al, 2001). In the regions where enteric fever is common, clinical diagnosis of typhoid fever is inadequate, as the symptoms it causes are non-specific and overlap with those of other febrile illness.…”

Section: Introductionmentioning

confidence: 99%

Prevalence, Detection and Antimicrobial Resistance Pattern of Salmonella in Sudan

Hussein¹,

Mohy-Eldin²,

Elmadiena³

et al. 2012

Salmonella - Distribution, Adaptation, Control Measures and Molecular Technologies

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Section: Introductionmentioning

confidence: 99%

Prevalence, Detection and Antimicrobial Resistance Pattern of Salmonella in Sudan

Hussein¹,

Mohy-Eldin²,

Elmadiena³

et al. 2012

Salmonella - Distribution, Adaptation, Control Measures and Molecular Technologies

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“…The assay combines simplicity with specificity and sensitivity in detecting organisms. PCR assay has been used successfully to diagnose the presence of bacterial pathogens in aquatic environments, food products and clinical samples (Chen and Griffiths, 2001;Ellingson et al, 2004;Guo et al, 2000;Myint et al, 2006;Patel et al, 2006;Sandery et al, 1996;Schrank et al, 2001;Soumet et al, 1999(a);Soumet et al, 1999(b) detection. Pre-enrichment of samples is thus necessary to lower the detection limit and dilute any inhibitory substances present in the samples.…”

Section: Introductionmentioning

confidence: 99%

Optimisation of the PCR-invA primers for the detection of Salmonella in drinking and surface waters following a pre-cultivation step

Moganedi¹,

Goyvaerts²,

Venter³

et al. 2009

WSA

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A polymerase chain reaction (PCR)-based method for the detection of Salmonella species in water samples was optimised and evaluated for speed, specificity and sensitivity. Optimisation of Mg 2+ and primer concentrations and cycling parameters increased the sensitivity and limit of detection of PCR to 2.6 x 10 4 cfu/mℓ. A 6h non-selective pre-enrichment step further increased the limit of detection to 26 cfu/mℓ. Out of 14 different Salmonella strains tested, only two, Salmonella arizonae and Salmonella pullorum, did not give positive amplification results with primers homologous to a conserved region of the invA gene. When environmental and drinking waters were assessed, a non-selective pre-enrichment step was included to increase the detection efficiency of PCR. The PCR method demonstrated specificity in the presence of other competing micro-organisms as confirmed by the conventional culture method. No false positives or negatives were observed when household and environmental water samples were tested by invA-PCR analysis parallel to the culture method.

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“…However, sample preparation prior to PCR is necessary, especially for feces, which contain high amount of compounds that are inhibitory for PCR (19). In spite of same enrichment broths to Salmonella are inhibitory for PCR (18), combination between different selective enrichment broths and PCR methods has been tested in order to improve sensitivity and dilute PCR inhibitory substances (6,15,18). The decision about the best methodology to be used to detect Salmonella in feces is very difficult due to the large amount of data that have been published.…”

Section: Introductionmentioning

confidence: 99%

Comparison of DNA-extraction methods and Selective Enrichment broths on the detection of Salmonella Typhimurium in swine feces by polymerase chain reaction (PCR)

Freschi¹,

Carvalho²,

Oliveira³

2005

Braz. J. Microbiol.

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The aim of this study was to compare different DNA-extraction methods and selective enrichment broths for their effectiveness to detect Salmonella Typhimurium in artificially inoculated swine feces samples (100 CFU/ g) by polymerase chain reaction. After enrichment in Rappaport-Vassiliadis, selenite cystine or Müller-Kauffmann tetrathionate, aliquots were used for DNA extraction by three different methods: boilingcentrifugation, phenol-chloroform and salting-out. Aliquots of extracted DNA were then used as template in PCR. The selective enrichment broths had no effect on the efficiency of PCR when boiling-centrifugation and salting-out were used. On the other hand, phenol-chloroform was superior (P<0.05) when combined to Rappaport-Vassiliadis. Considering cost and efficiency parameters, we encourage the use of Müller-Kauffmann tetrathionate broth in combination with boiling-centrifugation DNA-extraction procedure.

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Influence of enrichment media and application of a PCR based method to detect Salmonella in poultry industry products and clinical samples

Cited by 53 publications

References 19 publications

Prevalence, Detection and Antimicrobial Resistance Pattern of Salmonella in Sudan

Prevalence, Detection and Antimicrobial Resistance Pattern of Salmonella in Sudan

Optimisation of the PCR-invA primers for the detection of Salmonella in drinking and surface waters following a pre-cultivation step

Comparison of DNA-extraction methods and Selective Enrichment broths on the detection of Salmonella Typhimurium in swine feces by polymerase chain reaction (PCR)

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