Influence of enrichment and isolation media on the detection of Campylobacter spp. in naturally contaminated chicken samples

Repérant, E.; Laisney, Marie-José; Nagard, Bérengère; Quesne, Segolénè; Rouxel, Sandra; Gall, Françoise Le; Chemaly, Marianne; Denis, Martine

doi:10.1016/j.mimet.2016.06.028

Cited by 14 publications

(17 citation statements)

References 28 publications

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“…The use of different isolation methods, in particular regarding possibly varying inoculum size (Hsieh et al, 2018), enrichment (Carrillo et al, 2014;Reperant et al, 2016), temperature, media (Hsieh et al, 2018), and number of isolates identified at the species level (Carrillo et al, 2014;Reperant et al, 2016), may strongly influence the recovery of Campylobacter spp. from samples, the proportions of C. jejuni or C. coli or even the diversity of the isolates, including their profiles of antimicrobial susceptibility.…”

Section: 2mentioning

confidence: 99%

“…Those factors may vary between countries and likely yield differences. The observed proportions of Campylobacter species among isolates however also depend on the isolation methods, as certain media may favour one Campylobacter species over another (Reperant et al, 2016). The identification of a low number of colonies at the species level in a sample will, in most cases, result in the detection of the dominant species.…”

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confidence: 99%

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Technical specifications on harmonised monitoring of antimicrobial resistance in zoonotic and indicator bacteria from food‐producing animals and food

Aerts¹,

Battisti²,

Hendriksen³

et al. 2019

EFS2

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Proposals to update the harmonised monitoring and reporting of antimicrobial resistance (AMR) from a public health perspective in Salmonella, Campylobacter coli, Campylobacter jejuni, Escherichia coli, Enterococcus faecalis, Enterococcus faecium and methicillin-resistant Staphylococcus aureus (MRSA) from food-producing animals and derived meat in the EU are presented in this report, accounting for recent trends in AMR, data collection needs and new scientific developments. Phenotypic monitoring of AMR in bacterial isolates, using microdilution methods for testing susceptibility and interpreting resistance using epidemiological cut-off values is reinforced, including further characterisation of those isolates of E. coli and Salmonella showing resistance to extended-spectrum cephalosporins and carbapenems, as well as the specific monitoring of ESBL/AmpC/carbapenemase-producing E. coli. Combinations of bacterial species, food-producing animals and meat, as well as antimicrobial panels have been reviewed and adapted, where deemed necessary. Considering differing sample sizes, numerical simulations have been performed to evaluate the related statistical power available for assessing occurrence and temporal trends in resistance, with a predetermined accuracy, to support the choice of harmonised sample size. Randomised sampling procedures, based on a generic proportionate stratified sampling process, have been reviewed and reinforced. Proposals to improve the harmonisation of monitoring of prevalence, genetic diversity and AMR in MRSA are presented. It is suggested to complement routine monitoring with specific cross-sectional surveys on MRSA in pigs and on AMR in bacteria from seafood and the environment. Whole genome sequencing (WGS) of isolates obtained from the specific monitoring of ESBL/AmpC/carbapenemase-producing E. coli is strongly advocated to be implemented, on a voluntary basis, over the validity period of the next legislation, with possible mandatory implementation by the end of the period; the gene sequences encoding for ESBL/AmpC/carbapenemases being reported to EFSA. Harmonised protocols for WGS analysis/ interpretation and external quality assurance programmes are planned to be provided by the EU-Reference Laboratory on AMR.

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Section: 2mentioning

confidence: 99%

mentioning

confidence: 99%

Technical specifications on harmonised monitoring of antimicrobial resistance in zoonotic and indicator bacteria from food‐producing animals and food

Aerts¹,

Battisti²,

Hendriksen³

et al. 2019

EFS2

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“…This suggests that both direct plating and enrichment are useful in detecting Campylobacter [ 5 , 15 ]. After inhibition of low or high levels of competing bacteria, the enrichment method showed a higher detection rate in the present study, suggesting that enrichment in a selective medium is likely to be advantageous over the direct plating method [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of potassium clavulanate supplementation of Bolton broth for enrichment and detection of Campylobacter from chicken

2018

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Culture-based detection of Campylobacter can be affected by competing flora, temperature, incubation time, and presence of blood. The presence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli in poultry has become one of the most common factors interfering with the detection of Campylobacter. In the present study, we evaluated potassium clavulanate (ESBL inhibitor) as a supplement in Bolton broth (C-Bolton broth) for enrichment and detection of Campylobacter. First, we determined growth kinetics of Campylobacter in the presence of different concentrations of ESBL E. coli in C-Bolton broth during enrichment. The effects of other factors such as incubation time, incubation temperature, and presence of blood on Campylobacter detection in C-Bolton broth were also investigated. The growth of Campylobacter co-cultured at a low concentration (2 and 4 log10 CFU/mL) of ESBL E. coli was similar to that of Campylobacter alone in C-Bolton broth, and Campylobacter co-cultured at a high concentration (6 and 8 log10 CFU/mL) of ESBL E. coli showed slower growth than the pure Campylobacter culture. The Campylobacter detection limit was 1 log10 CFU/mL when mixed with 2, 4, or 6 log10 CFU/mL of E. coli and 3 log10 CFU/mL when mixed with 8 log10 CFU/mL of E. coli after 48 h enrichment in the broth. Campylobacter detection from chicken feces and litter samples was not affected by incubation time, or presence of blood in the broth. A modified procedure of enrichment in C-Bolton broth at 37°C for 24 h without blood showed a significantly (P ≤ 0.05) higher detection rate and a lower false-negative rate than the ISO 10272–1:2006 method for Campylobacter detection from chicken feces and litter samples. In summary, the present study demonstrates the efficacy of Bolton broth supplemented with potassium clavulanate in the detection of Campylobacter mixed with ESBL E. coli, and an improved procedure to detect Campylobacter from chicken feces and litter samples.

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“…It has well recognized that the recovery of Campylobacter requires selective media, due to their inability to compete with other germs (Josefsen et al, 2003;Repérant et al, 2016). Today, there is a wide range of plating media, whose selectivity differs from each other's, and usually grouped in two categories; this includes blood-based agar (Preston, Skirrow, Butzler, Campy-cefex agar) and others based on charcoal instead of blood, such as Karmali and modified charcoal cefoperazone deoxycholate agar (mCCDA).…”

Section: Culture Mediamentioning

confidence: 99%

A Review of Current Knowledge and Gaps About Campylobacter Methods: From Culture to Characterization

Baaboua¹,

Maadoudi²,

Bouyahya

et al. 2022

J microb biotech food sci

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Campylobacteriosis is a zoonotic disease caused by Campylobacter, mostly associated with consumption of contaminated foodstuffs and water. Campylobacter jejuni and Campylobacter coli recognized as the leader of foodborne diarrheal illness in humans. The frequency of these microorganisms in poultry is fairly high than Salmonella and more challenging to measure, which represent an expensive burden charge on public health due to their difficulties to master them, especially with the fast increase rates of multidrug-resistant of thermophilic Campylobacter strains. It is well recognized that Campylobacter spp. is a fastidious cell, difficult to isolate in laboratories owing to their requirements and sensibility. That’s why; these factors must be taken into consideration during recovery protocols. A variety of phenotyping tests have been reported and widely used for confirmation and identification of Campylobacter species. Nonetheless, Whole Genome Sequencing (WGS) and Culture-Independent Diagnostic Tests (CIDTs) are new eras of hopeful technologies, mainly involved in the detection and characterization of threaten public health pathogens. This review aimed to describe the culture methods, phenotypic and genotypic schemes used to isolate, identify, and characterize Campylobacter isolates, through discussing the current knowledge and gaps related to the application of these techniques over others performed for typing this microaerophilic genus.

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Influence of enrichment and isolation media on the detection of Campylobacter spp. in naturally contaminated chicken samples

Cited by 14 publications

References 28 publications

Technical specifications on harmonised monitoring of antimicrobial resistance in zoonotic and indicator bacteria from food‐producing animals and food

Technical specifications on harmonised monitoring of antimicrobial resistance in zoonotic and indicator bacteria from food‐producing animals and food

Evaluation of potassium clavulanate supplementation of Bolton broth for enrichment and detection of Campylobacter from chicken

A Review of Current Knowledge and Gaps About Campylobacter Methods: From Culture to Characterization

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