Influence of electrohydrodynamic jetting parameters on the morphology of PCL scaffolds

Abstract

Abstract:One of the important constituents in tissue engineering is scaffold, which provides structural support and suitable microenvironment for the cell attachment, growth and proliferation. To fabricate micro/nano structures for soft tissue repair and three-dimensional (3D) cell culture, the key is to improve fibre-based scaffold fabrication. Electrohydrodynamic (EHD) jetting is capable of producing and orientating submicron fibres for 3D scaffold fabrication. In this work, an EHD-jetting system was developed to explore the relationship between vital processing parameters and fibre characteristics. In this study, polycaprolactone (PCL) solution prepared by dissolving PCL pellets in acetic acid was used to fabricate the scaffolds. The influence of voltage, motorized stage speed, solution feed rate, and solution concentration on fibre characteristics and scaffold pattern were studied. Morphology of the EHD-jetted PCL fibres and scaffolds were analysed using optical microscope images and scanning electron microscope (SEM) images. Multi-layer scaffolds with the varied coiled pattern were fabricated and analysed. Cell attachment and proliferation have to be investigated in the future by further cell culture studies on these multi-layer coiled scaffolds.

3D‐Printed PCL/rGO Conductive Scaffolds for Peripheral Nerve Injury Repair

“…The dependence of scaffold morphology on these parameters was studied and reported before (28). EHD-jet 3D printing is a high-resolution process and a number of polymers could be processed using this technique.…”

“…Nonetheless, the disadvantages of electrospinning include the inability to precisely control the scaffold morphology including pore size and porosity, lack of geometrical freedom, and non-scalability. Electrohydrodynamic jet (EHDjet) 3D printing works on the same principle as electrospinning but overcomes the limitations of the latter (28)(29)(30). EHD-jet 3D printing offers geometric freedom like other additive manufacturing processes and the scaffolds are printed layer by layer.…”

Emerging Trends in Information‐Driven Engineering of Complex Biological Systems

“…[170] As the needle to collector distance is fixed to 500 µm-1 mm, the solvent in the polymer jet may not have sufficient time to fully evaporate and may remain liquid after deposition. [172] Zhou et al investigated the effect of various parame ters, such as working distance, flow rate, and stage speed on the morphology of PCL fibers and sugar-PCL core-shell fibers with similar microstructures to neuronal and muscle tissues. [172] Zhou et al investigated the effect of various parame ters, such as working distance, flow rate, and stage speed on the morphology of PCL fibers and sugar-PCL core-shell fibers with similar microstructures to neuronal and muscle tissues.…”

“…[172] Zhou et al investigated the effect of various parame ters, such as working distance, flow rate, and stage speed on the morphology of PCL fibers and sugar-PCL core-shell fibers with similar microstructures to neuronal and muscle tissues. [172] Nevertheless, the height of the scaffolds produced with NFES is limited by the short working distance. [172] Nevertheless, the height of the scaffolds produced with NFES is limited by the short working distance.…”

Cell powered biobots and more perspectives for IJB

“…Sing et al investigate titanium/tantalum/ collagen-based biphasic implants for repairing osteochondral defects [6] . Liu et al study the morphology of polycaprolactone scaffold made by electrohydrodynamic jetting [7] . Suntornnond provides a perspective on the role of support materials in bioprinting [8] .…”