Influence of DNA repair defects (radl, rad52) on nitrogen mustard mutagenesis in yeast

Abstract

Nitrogen mustard (HN2) mutagenesis of a plasmid-borne copy of the Saccharomyces cerevisiae SUP4-o gene was examined in a repair-proficient yeast strain and isogenic derivatives defective for excision (rad1) or DNA double-strand break (rad52) repair. The excision repair deficiency sensitized the cells to killing by HN2 and abolished mutation induction. Inactivation of RAD52 had no influence on the lethality of HN2 treatment but diminished the induced mutation frequency by 50% at all doses tested. DNA sequence analysis of HN2-induced SUP4-o mutations suggested that RAD52 contributed to the production of basepair substitutions at G.C sites. The rad52 defect appeared to alter the distribution of G.C-->A.T transitions in SUP4-o relative to the distribution for the wild-type strain. This difference did not seem to be due to an effect of RAD52 on the relative fractions of HN2-induced transitions at localized (flanked by A.T pairs) or contiguous (flanked by at least one G.C pair) G.C sites but instead to an influence on the strand specificity of HN2 mutagenesis. In the repair-proficient strain, the transitions showed a small bias for sites having the guanine on the transcribed strand and this preference was eliminated by inactivation of RAD52.

Delayed haematological complications of mustard gas

“…Although some of these complications are transient or treatable in the early phases, delayed complications may remain for years. Alkylating effects of mustard gas disturb the DNA of haematopoietic cells (Mis and Kunz, 1992;Watson and Griffin, 1992). High-dose exposure has a cytotoxic effect on haematopoietic stem cells and pancytopenia has been seen in Iranian combatants (Tabarestani, 1988).…”