Influence of different surface modification treatments on poly(d,l-lactic acid) with silk fibroin and their effects on the culture of osteoblast in vitro

Cai, Kaiyong; Yao, Kangde; Cui, Yuan‐Lu; Yang, Zhiming; Li, Xiuqiong; Xie, Huiqi; Qing, Tingwu; Gao, Laibao

doi:10.1016/s0142-9612(01)00287-3

Cited by 154 publications

(90 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although porosity and pore size distribution are important parameters for successful cell-biomaterial interactions, the results of the present study suggest that the closing of some pores caused by collagen coating did not adversely affect the cytocompatibility and performance of modified scaffolds. This finding is in accordance with other reports that mention the difficulty in controlling the extent of acrylic acid grafting and covalent collagen immobilization used to modify surface properties 24,26 .…”

Section: Discussionsupporting

confidence: 93%

“…Acrylic acid grafting generated nano-and micropores on the membrane and scaffold surfaces. However, the micropores in the scaffolds were originated mainly in the pore walls, where the polymeric matrix was weaker and more susceptible to chain scission by acid hydrolysis during acrylic acid grafting 24,25 . Coating the surface with collagen resulted in the obstruction of some pores.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Surface characterization and osteoblast-like Cells culture on collagen modified PLDLA scaffolds

et al. 2014

View full text Add to dashboard Cite

Surface modification techniques based on the grafting of chemical functional groups and immobilization of bioactive molecules have been used to improve biocompatibility and clinical performance of bioabsorbable scaffolds in tissue engineering and medicine regenerative applications. This study aimed at developing and characterizing a biomimetic surface to stimulate bone regeneration by a simple and low-cost method of surface biofunctionalization of the poly (L-co-D,L lactic acid)-PLDLA scaffolds. The method was obtained by grafting reaction of carboxyl groups (-COOH) on their surface via acrylic acid (AAc) polymerization process, followed by immobilization of collagen type I (Col). Such approach resulted in a surface morphology markedly modified after treatment, with increase of pores and roughness on PLDLA-AAc surfaces and a network of fibrillar collagen deposition in nonspecific areas of PLDLA-Col surfaces. The cytocompatibility of collagen-immobilized scaffolds was significantly improved in terms of cellular adhesion, proliferation, collagen synthesis and maintenance of osteoblast-like phenotype, indicating, therefore, the fundamental role of collagen protein over the biological interactions that occur by bio-recognition mimetic mechanisms at biomaterials interface. These results indicate that the surface modification method used here may be useful as a strategy to develop biofunctional scaffolds, which provide a more successful clinical application of biomaterials in the tissue engineering field.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Surface characterization and osteoblast-like Cells culture on collagen modified PLDLA scaffolds

et al. 2014

View full text Add to dashboard Cite

show abstract

“…30 It was also used as composite coating with calcium phosphate/ hydroxyapatite 31 on titanium substrates because of its good biocompatibility. In our previous studies, 32,33 silk fibroin was employed to surface modify poly (D,L-lactic acid), which confirmed silk fibroin having beneficial effects on improving osteoblast growth behaviors. Although the layer-by-layer technique has been applied on titanium oxide 34,35 previously, studies mainly focused on the production of composite with titanium oxide.…”

Section: Introductionmentioning

confidence: 79%

Surface engineering of titanium thin films with silk fibroin via layer‐by‐layer technique and its effects on osteoblast growth behavior

Cai

Jandt

2007

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

The objective of the present study was to surface modify the titanium thin films to improve its biocompatibility. A layer-by-layer (LBL) self-assembly technique, based on the electrostatic interactions mediated adsorption of chitosan (Chi) and silk fibroin (SF), was used leading to the formation of multilayers on the titanium thin film surfaces. The surface chemistry and wettability of LBL films were investigated by X-ray photoelectron spectroscopy (XPS), water contact angle measurement, and atomic force microscopy, respectively. XPS and contact angle measurement results indicated that a full SF/Chi pair film was formed after the deposition layers of PEI/(SF/Chi)(2) on the titanium film surfaces. The topographies of multilayered films were directly related to the corresponding outmost layer components. The build-up of such SF/Chi pair films on titanium films may in turn affect the biocompatibility of the modified titanium films. Therefore, an in vitro investigation was performed to confirm this hypothesis. Cell proliferation, cell viability, DNA synthesis as well as differentiation function (alkaline phosphatase) of osteoblasts on LBL-modified titanium films and control samples were investigated, respectively. Osteoblasts cultured on modified titanium films was found to be higher proliferation tendency than that on control (p < 0.05). Cell viability, alkaline phosphatase as well as DNA synthesis measurement indicated that osteoblasts on LBL-modified films were greater (p < 0.05 or p < 0.01) than the control, respectively. These results suggest that surface engineering of titanium was successfully achieved via LBL deposition of Chi/SF pairs, and enhanced its cell biocompatibility. The approach presented in the study may be exploited as an efficient alternative for surface engineering of titanium-based implants.

show abstract

“…In general, the Amide I mode of silk fibroin associating with the α-form conformation appears at 1650-1660 cm −1 , whereas the random coil conformation at 1640-1650 cm −1 and the β-form conformation at 1620 and 1640 cm −1 ; the Amide II mode of silk fibroin appear at 1520-1530 cm −1 . Figure 4 represents the ATR-FTIR spectra of PHBHHx porous scaffold (Figure 4(A)), SF-modified scaffold without (Figure 4 [24]. Notably, the peak at 1653 cm −1 (Figure 4(B)) indicates that the silk fibroin conformation is α-form before the scaffold is treated by methanol.…”

Section: Adhesion Ability Of Silkmentioning

confidence: 99%

Degradable PHBHHx Modified by the Silk Fibroin for the Applications of Cardiovascular Tissue Engineering

Yang

Sun

Zhang

et al. 2011

ISRN Materials Science

View full text Add to dashboard Cite

Biodegradable polymer, poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx), has been used to fabricate the tissueengineered cardiovascular scaffolds due to its controllable and suitable mechanical properties. However, the unsound biocompatibility of PHBHHx is still the unsolved problem till now. Silk fibroin (SF) with low inflammation, no blood clotting, and good cell and tissue compatibility in vitro and vivo is adopted as a surface modificator to improve the biocompatibility of PHBHHx. The adhesion of SF on PHBHHx surface was investigated by infrared spectroscopy and water contact angle. The proliferation and morphologies of human fibroblasts, human smooth muscle cells, endothelial-like cell line ECV304, and umbilical vein endothelial cells on the SF-modified PHBHHx were studied by the assays of MTT, H&E staining, DAPI staining, and the scanning electron microscopy. In sum, SF-modified PHBHHx scaffolds are highly biocompatible with cardiovascular-related cells, demonstrating its potential help for the extensive applications of PHBHHx in the cardiovascular regeneration.

show abstract

Influence of different surface modification treatments on poly(d,l-lactic acid) with silk fibroin and their effects on the culture of osteoblast in vitro

Cited by 154 publications

References 23 publications

Surface characterization and osteoblast-like Cells culture on collagen modified PLDLA scaffolds

Surface characterization and osteoblast-like Cells culture on collagen modified PLDLA scaffolds

Surface engineering of titanium thin films with silk fibroin via layer‐by‐layer technique and its effects on osteoblast growth behavior

Degradable PHBHHx Modified by the Silk Fibroin for the Applications of Cardiovascular Tissue Engineering

Contact Info

Product

Resources

About