Influence of Different Concentration of Tris Buffer Solution on Calcium Carbonate Precipitation in Bio-based Repair Materials

Putri, Prima Yane; Kawaai, Keiyu; Ujike, Isao; Okuno, Hiroshi

doi:10.18517/ijaseit.7.5.3452

Cited by 4 publications

(6 citation statements)

References 11 publications

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“…Based on the result of previous experiments carried out by Putri et al [24], the highest calcium carbonate precipitates were found to the concentration of tris buffer solution in the range of 0.5 to 0.75 (mol/L) for initial pH adjusted to 9.0. The concentration of dry yeast were specified as 27 g/L, which were mixed with glucose and calcium acetate with concentrations of 0.3 mol/L and 0.15 mol/L respectively.…”

Section: Application Of Bio-based Repair Materialsmentioning

confidence: 91%

“…Based on previous research [24], amount of precipitates is mainly comprising calcium carbonate up to 48 h after they were mixed which seemed to reach about 0.388 g in the 30 ml test tube. The volume of the gap is 68.292 cm 3 and based on the calculation, required 5280.3 mL of bio-based materials to fill the leakage for 0.6 mm crack width.…”

Section: Casementioning

confidence: 92%

“…Putri, et al [22]- [24] observed precipitation of calcium carbonate caused by metabolic activity of stagnant bio-based material in the test tubes. The precipitates are mainly comprised of calcium carbonate up to 48h after mixed.…”

Section: Introductionmentioning

confidence: 99%

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Application of bio-based material for concrete repair: case study leakage on parallel concrete slab

2019

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The applicability of bio-based materials for concrete repair has been studied. This technique employs yeast, glucose and calcium acetate mixed in Tris buffer solution. The microbial metabolic process leads to precipitation of calcium carbonate. First, this study investigated the applicability of bio-based repair materials to small-scale concrete specimens. On this research, water permeability test was carried out to evaluate the effectiveness of the selected mixtures for sealing cracks in the concrete specimens. As the result of permeability tests carried out using specimens with crack width of 0.6 mm, water leakage through crack was observed to be negligible after 216 hours by continuous pouring method using bio-based repair materials. Also, this study showed the initial flow rate for the specimens with the same crack width does not influence crack sealing time. Furthermore, the precipitation of the calcium carbonate from the bio-based materials was analyzed by Fourier-Transformed Infra-Red spectroscopy (FT-IR) and then examined by X-ray Diffraction (XRD) for mineral identification formed through the microbial metabolic process.

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Section: Application Of Bio-based Repair Materialsmentioning

confidence: 91%

Section: Casementioning

confidence: 92%

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Application of bio-based material for concrete repair: case study leakage on parallel concrete slab

2019

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“…According to Kawaai [19] and Putri [30][31][32], the initial pH levels and concentration of the mixture influence the precipitation rate, particularly at the beginning of the reaction. Correspondingly, the most effective compositions in the early phases contribute to increasing the amount of precipitation, as well as speeding up the reaction rate due to the effectiveness of sealing the leakage in concrete.…”

Section: Effect Of Different Concentrations Of Yeast-based Mixtures On Calcium Carbonate Precipitationmentioning

confidence: 99%

Calcium Carbonate in Bio-Based Material and Factor Affecting Its Precipitation Rate for Repairing Concrete

et al. 2020

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The use of bio-based material for repairing concrete is a relatively new method. Therefore, more results from simulated real-condition experiments are needed before being applied on a practical scale. In the recent past, several studies have been conducted on the improvement of bio-based repair materials. In this study, the bio-based material involving yeast, glucose, and calcium acetate mixed in a Tris buffer solution showed the potential to develop a microbial process leading to the precipitation of calcium carbonate. We investigated the factors affecting the precipitation rate of the calcium carbonate of bio-based materials for repairing leakage in the concrete specimens. Based on a series of experiments involving temperature, the type of dry yeast, and the concentration of the Tris buffer solution, the composition of bio-based materials with the highest precipitation rate of calcium carbonate was selected. The selected mixture could be applied to repair leakage of concrete until the cracks are sealed entirely.

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“…Putri et al [14] also investigated the effect of dry yeast types on calcium carbonate precipitation rates in Japan. When dry yeast is selected as the microorganism, carbon dioxide, produced through microbial metabolic processes (by consuming an organic carbon source such as glucose), provides carbonate ions.…”

Section: Introductionmentioning

confidence: 99%

EFFECT OF TEMPERATURE AND SOLUTION pH ON CALCIUM CARBONATE PRECIPITATION AS BIO-BASED REPAIR MATERIAL IN CONCRETE

Putri

2024

GEOMATE

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In the present work, the effect of temperature and solution pH on calcium carbonate precipitation from bio-based repair materials is investigated. A microbial metabolic process precipitates calcium carbonate. Mixtures of bio-based materials typically comprise a microorganism, an organic carbon source, and a calcium source. When dry yeast is selected as the microorganism, carbon dioxide produced through the microbial metabolic process of consuming an organic carbon source, such as glucose, provides carbonate ions. The carbonate ions lead to reactions with the calcium ions present in the mixture, leading to the precipitation of calcium carbonate. Mixtures were tested at temperature conditions of 10, 20, and 30 ºC, and the pH of the solution was initially adjusted as 8.0 or 9.0, for up to 72 hours of elapsed time. In this research, temperature conditions of 20-30 °C is the optimum temperature range for increasing the precipitation of calcium carbonate. The results confirmed that the maturity index has a linear correlation with the decreasing rate of calcium ions. Large maturity indices mean that the decreasing rate of calcium ions is high. Maturity index was used as an indicator of the precipitation rate of calcium carbonate in the temperature range 10-30 o C; the datum temperature was 0 o C. The adequate concentration of the Tris buffer solution was found in the range 0.50-0.75 (mol/L), at an initial pH of 9.0, and 0.75-1.00 (mol/L), at an initial pH of 8.0. The rate of the initial reaction tended to be faster with the initial pH of 9.0.

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Influence of Different Concentration of Tris Buffer Solution on Calcium Carbonate Precipitation in Bio-based Repair Materials

Cited by 4 publications

References 11 publications

Application of bio-based material for concrete repair: case study leakage on parallel concrete slab

Application of bio-based material for concrete repair: case study leakage on parallel concrete slab

Calcium Carbonate in Bio-Based Material and Factor Affecting Its Precipitation Rate for Repairing Concrete

EFFECT OF TEMPERATURE AND SOLUTION pH ON CALCIUM CARBONATE PRECIPITATION AS BIO-BASED REPAIR MATERIAL IN CONCRETE

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