Influence of different carbon sources on bacterial cellulose production byGluconacetobacter xylinusstrain ATCC 53524

Abstract

Aims: To determine the effect of carbon sources on cellulose produced by Gluconacetobacter xylinus strain ATCC 53524, and to characterize the purity and structural features of the cellulose produced. Methods and Results: Modified Hestrin Schramm medium containing the carbon sources mannitol, glucose, glycerol, fructose, sucrose or galactose were inoculated with Ga. xylinus strain ATCC 53524. Plate counts indicated that all carbon sources supported growth of the strain. Sucrose and glycerol gave the highest cellulose yields of 3·83 and 3·75 g l−1 respectively after 96 h fermentation, primarily due to a surge in cellulose production in the last 12 h. Mannitol, fructose or glucose resulted in consistent rates of cellulose production and yields of >2·5 g l−1. Solid state 13C CP/MAS NMR revealed that irrespective of the carbon source, the cellulose produced by ATCC 53524 was pure and highly crystalline. Scanning electron micrographs illustrated the densely packed network of cellulose fibres within the pellicles and that the different carbon sources did not markedly alter the micro‐architecture of the resulting cellulose pellicles. Conclusions: The production rate of bacterial cellulose by Ga. xylinus (ATCC 53524) was influenced by different carbon sources, but the product formed was indistinguishable in molecular and microscopic features. Significance and Impact of the Study: Our studies for the first time examined the influence of different carbon sources on the rate of cellulose production by Ga. xylinus ATCC 53524, and the molecular and microscopic features of the cellulose produced.

Bacterial Cellulose Production by Komagataeibacter hansenii ATCC 23769 Using Sisal Juice - An Agroindustry Waste

“…Yield decreased from 66% to 30% from the 2 nd to the 4 th day of culture of G. xylinus ATCC 53524 in HS and the most significant increase happened in the first 4 days of culture. BC production by G. xylinus ATCC 53524 in HS medium ranged from 1.89 to 3.10 g/l between the second and fourth day of cultivation (Mikkelsen et al, 2009). It was similar to that obtained for G. hansenii ATCC 23769 in SM and HS medium in this work.…”

Pectin and Xyloglucan Influence the Attachment of Salmonella enterica and Listeria monocytogenes to Bacterial Cellulose-Derived Plant Cell Wall Models

“…Our BC composites were shown to have compositions similar to those of other BC-based PCW models reported in the literature (34,35). Our BCPX composites also showed chemical composi- tions similar to those of average native PCWs (approximately 35% cellulose, 40% pectin, and 15% xyloglucan) (36).…”

The Effect of Carbon and Nitrogen Sources on Bacterial Cellulose Production and Properties from Gluconacetobacter sucrofermentans CECT 7291 Focused on its use in Degraded Paper Restoration

“…sucrofermentans is a rod-shaped, obligate aerobic, gram-negative bacterium (Kersters et al 2006). BC synthesized by G. sucrofermentans has specific physical and chemical properties that distinguish it from plant cellulose, such as high crystallinity carbon substrates for the G. xylinus strain ATCC 53524 (Mikkelsen et al 2009); sucrose also gave the best result with Acetobacter sp. 4B-2 (Pourramezan et al 2009); glycerol was the best substrate for G. xylinus ATCC 10245 (Keshk and Sameshima 2005); and glucose was found to be the best carbon source for Acetobacter lovaniensis HBB5 (Çoban and Biyik 2011).…”