Influence of dietary fish oil supplementation on humoral immune response and some selected biochemical parameters of broiler chickens

Abstract

Influence of dietary fish oil supplementation on humoral immune response and some selected biochemical parameters of broiler chickensThe objective of this experiment was to evaluate the influence of fish oil (FO) supplementation in the diet of broiler chickens on the humoral immune response as well as some blood parameters. Two hundred and sixteen one day old broiler chickens were divided into four dietary groups 0, 1, 2, or 4% FO with 3 replicates of 18 birds. Four chicks randomly selected and marked from each replicate were immunized intramuscularly with 0.2 ml of 5% sheep red blood cells (SRBC) as a non-infectious antigen, at the ages of 15 and 35 days and blood samples were taken 7 days after each immunization. The highest BW was observed in the 2% FO dietary group (T3), followed by T2 (P<0.01). The serum cholesterol and triglyceride levels significantly decreased in the FO groups at the age of 42 days (P<0.01). In addition, the inclusion of FO in broiler diets significantly increased the blood glucose (G) level and decreased the total protein (TP), albumin (A) and globulin (GL) concentrations. Fish oil-treated birds had significantly more serum antibody (predominantly immunoglobulin M, IgM) to SRBC than the control group. The highest response to primary and secondary injections of SRBC after 7 days, were detected for group 4 (4% FO), followed by 2% FO group (P<0.05). The results indicate that the addition of 2 % FO to broiler chick's diet may stimulate the development of the immune response and improve blood indices, while 4% level was not recommended because of probable off-flavours in the product.

Clove bud (Syzygium aromaticum) improved blood and hepatic antioxidant indices in laying hens receiving low n‐6 to n‐3 ratios

“…This might be due to the fact that omega‐3 fatty acids reduce triglyceride synthesis rates and raise its removal by peripheral tissues and liver (Crespo and Esteve‐Garcia, ). In this case, the presence of PUFA from direct deposit of the diet or conversion of precursors by de novo synthesis in the liver or tissues might be responsible for a decline in serum triglyceride and cholesterol contents (Hosseini‐Mansoub and Bahrami, ). Besides, a decrease in serum triglyceride content by feeding n‐3 polyunsaturated fatty acids might be in part due to an alteration in the fluidity and composition of the plasma cell membrane (Hosseini‐Mansoub and Bahrami, ).…”

“…In this case, the presence of PUFA from direct deposit of the diet or conversion of precursors by de novo synthesis in the liver or tissues might be responsible for a decline in serum triglyceride and cholesterol contents (Hosseini‐Mansoub and Bahrami, ). Besides, a decrease in serum triglyceride content by feeding n‐3 polyunsaturated fatty acids might be in part due to an alteration in the fluidity and composition of the plasma cell membrane (Hosseini‐Mansoub and Bahrami, ). Confirming these findings, studies have demonstrated that intake of fish oil containing DHA and EPA reduced serum triglyceride concentrations in ducks (Liu et al., ) and hyperlipidaemic men (Mori et al., ).…”

Anticoccidial Effect of Herbal Powder “Shi Ying Zi” in Chickens Infected with Eimeria tenella

“…When infective sporozoites enter the cecum mucosa by penetrating villus epithelial cells, this leads to extensive destruction of the cecum epithelium, bloody stools, and a large amount of oocyst excretion [29,30]. In this study, infected chicks were dull and depressed, exhibiting disheveled feathers and a lower feed intake, which may have been due to altered gut homeostasis that led to poor feed intake and metabolism and thus decreased weight gains [31,32]. "Shi Ying Zi" powder can alleviate the histopathological changes of the cecum, and the number of oocysts and mucosa cell necrocytosis in the cecum were decreased.…”

Anticoccidial effect of "Shi Ying Zi" Powder in chickens infected with Eimeria tenella

“…When infective sporozoites enter the caecum mucosa by penetrating villus epithelial cells, leading to extensive destruction of the caecum epithelium, hemorrhagic feces output and a large number of oocysts excretion [19][20]. In this study, infected chicks were dull and depressed with ruffled feather and less feed intake, which may be due to altered gut homeostasis that leads to poor feed intake, metabolism and thus decreased weight gains [21,22]. "Shi Ying Zi" powder can alleviate the histopathological changes of caecum, and the number of oocysts and mucosa cell necrocytosis in caecum has decreased.…”