Influence of Dietary Calcium:Phosphorus Ratio on Mineral Excretion and Nephrocalcinosis in Female Rats

Hoek, A.; Lemmens, A. G.; Mullink, J. W. M. A.; Beynen, A.C.

doi:10.1093/jn/118.10.1210

Cited by 99 publications

(48 citation statements)

References 10 publications

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“…The Spearman rank correlation coefficients were 0·91 (P<O 'Ol,n=24) and 0'71 (P<O·Ol, n=36) for experiments 1 and 2, respectively. This association has also been found in previous studies (Hoek et al, 1988;Mars et al, 1988;Schoenmakers et al, 1989). Kidney calcification in rats fed the high-P diets containing 1000 IU of vitamin D/kg was associated with increased group mean plasma urea concentrations (see Tables 4 and 6), pointing to diminished kidney function.…”

Section: Resultssupporting

confidence: 88%

Vitamin D, within its range of fluctuation in commercial rat diets, does not influence nephrocalcinogenesis in female rats

et al. 1991

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SummaryMassive, toxic doses of vitamin D have been shown to cause nephrocalcinosis in rats, but the effect of this vitamin within its range of fluctuation in commercial rat diets was unknown. Therefore, in two experiments with young female rats, the effect on nephrocalcinosis of a moderately increased level of vitamin D in the diet was studied, that is 5000 IU /kg versus the recommended concentration of 1000 IV/kg. This was done using purified diets with 0·5% (w/w) calcium and O· 0411Jo magnesium containing either 0·2 or O' 611Jo phosphorus (P). Ratsfed the diets containing 0 '611Jo P showed severe kidney calcification compared to those fed the 0'211Jo-P diets. The level of vitamin D in the O· 2 and O' 611Jo-Pdiets did not affect kidney calcification. Bone density was increased after feeding diets containing 5000 instead of 1000 IV of vitamin D/kg. This study suggests that, within 28 days, a moderate increase of the amount of vitamin D in the diet has no influence on the development of kidney calcification. This in turn suggests that the variation in nephrocalcinosis severity and incidence seen in practice in rats fed different commercial diets is unlikely to be related to the different vitamin D concentrations in these diets. However, in rats fed such diets bone metabolism may be influenced differently.

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Section: Resultssupporting

confidence: 88%

Vitamin D, within its range of fluctuation in commercial rat diets, does not influence nephrocalcinogenesis in female rats

et al. 1991

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“…They reported that high phosphorus intake caused kidney calcification and an increase in kidney weight (22)(23)(24). Ritskes-Hoitinga and colleagues (25,26) reported that high phosphorus intake led to an increase in kidney weight due to calcification, but there was no kidney edema.…”

Section: Discussionmentioning

confidence: 99%

Effect of the Protein and Oil Contents on Calcium Utilization in the Diets of Female Fischer Rats That Were Fed by Three Different Feeding Methods

Ohtsuka

Kitano

Maeda

et al. 2006

J Nutr Sci Vitaminol

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SummaryWe studied the effects of varying the protein and oil contents in the diet and three different feeding methods on the utilization of dietary calcium (Ca) in female Fischer rats. The experimental diets were based on the AIN-76 diet. Rats were fed one of nine experimental diets containing different levels of protein and oil. The experimental diets contained 10, 20 or 40% of protein (milk casein) and 5, 10 or 20% of soybean oil. The three meal feeding methods were ad libitum feeding, pair feeding and adjustable feeding. The experimental diets that were given by prepared feeding were supplemented with a mineral and vitamin solution. The effects of the protein and oil contents in the diet were analyzed by two-way ANOVA. Among rats that were fed ad libitum, the oil content in the diet affected the degree of mineral and vitamin intake. Among the rats fed ad libitum, the oil content in the diet had a significant effect on the level of Ca intake, although it did not have a significant effect on the level of energy intake. For pair feeding, the feeding volume was limited in each group; therefore, there was a significant difference in energy intake and there was no significant difference in Ca intake among each diet group. For adjustable feeding, there was considerable mineral and vitamin intake, which effected the reduction of feeding volume depending on the oil volume in the diet. There were no significant differences in the energy, Ca and other mineral and vitamin intakes among the nine groups that were fed using adjustable feeding. In analyzing two-way ANOVA, in which the parameters were the contents of protein and oil in the experimental diets, there were differences in Ca utilization among rats that were fed using the three feeding methods. This result was shown to reflect on the difference as energy, mineral including Ca, and vitamin intake. However, the protein content in the diet had a significant effect on urinary Ca excretion in all three feeding methods. In addition, it was clear that the intake of protein and oil affected kidney calcification in all three feeding methods. The AIN Experimental Diet Committee reported that kidney calcification was found among rats that were fed the AIN-76 diet and that one of the causes of kidney calcification was the Ca/P ratio in this diet. The protein and oil contents in the diets had significant effects on the degree of kidney calcification among rats that were fed by pair feeding or adjustable feeding. The results of this study suggest that the protein and oil contents in the diet play an important role in kidney calcification. Key Words calcium balance, protein intake, oil intake, kidney calcification, feeding method There have been many nutrition studies on the bioavailability of minerals including calcium, phosphorus (P) and magnesium (Mg) balance studies in experimental animals such as rats. We previously studied the effect of varying the level of protein intake on calcium utilization and found that a high-protein diet increased the absorption and urinary excreti...

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“…The composition of the premixes has been described elsewhere (Hoek et al 1988) Composition of premixes has been described elsewhere (Hoek et al 1988) similar. Each dietary group thus consisted of four males and four females.…”

Section: Experiments With Dba/2 Micementioning

confidence: 99%

The influence of dietary phosphorus and magnesium concentrations on the calcium content of heart and kidneys of DBA/2 and NMRI mice

Broek

Beynen

1998

Lab Anim

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SummaryDystrophic cardiac calcification (DCC) is often found in DBA/2 mice, reportedly in association with low plasma magnesium levels in this mouse strain. It was hypothesized that high-phosphorus diets and low-magnesium diets that are known to lower plasma magnesium concentrations would promote the development of DCC. DBA/2 mice were fed diets with either low-magnesium (0.02%, w/wl or high-phosphorus (0.8%) concentrations or a combination of the two variables. NMRI mice were given either a low-(0.2%) or high-(0.6%) phosphorus diet.Female, but not male, NMRI mice accumulated calcium in the heart when fed the highphosphorus diet; neither gender developed kidney calcification. DBA/2 mice with either a low-magnesium or a high-phosphorus intake developed marked cardiac calcifications. The combination of low-magnesium and high-phosphorus intake caused severe calcification of the heart, kidney and tongue'. Increasing the dietary magnesium content (0.08%) and reducing phosphorus (0.2%) did not fully prevent cardiac calcification, but reduced heart calcium concentrations in male DBA/2 mice. It is concluded that diets for DCC-susceptib1e mice should contain adequate amounts of magnesium and low, but sufficient amounts of phosphorus in order not to additionally stimulate cardiac calcification.

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Influence of Dietary Calcium:Phosphorus Ratio on Mineral Excretion and Nephrocalcinosis in Female Rats

Cited by 99 publications

References 10 publications

Vitamin D, within its range of fluctuation in commercial rat diets, does not influence nephrocalcinogenesis in female rats

Vitamin D, within its range of fluctuation in commercial rat diets, does not influence nephrocalcinogenesis in female rats

Effect of the Protein and Oil Contents on Calcium Utilization in the Diets of Female Fischer Rats That Were Fed by Three Different Feeding Methods

The influence of dietary phosphorus and magnesium concentrations on the calcium content of heart and kidneys of DBA/2 and NMRI mice

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