Influence of Diet on the Bioavailability of Active Components from Zingiber officinale Using an In Vitro Digestion Model

Abstract

Ginger (Zingiber officinale Rosc.) is a plant known all over the world that is used as a spice and as an ingredient in drinks, dietary supplements, and cosmetics. The growing availability of its fresh rhizomes makes it even more likely to be used in the diet, mainly due to its beneficial health properties and high content of polyphenols (gingerols and shogaols). The main goal and motivation of the authors was to assess the bioavailability of active substances contained in the extract from ginger rhizomes in the presence of various types of diets using the in vitro digestion method, enabling simulation of the processes occurring during the digestion and absorption of metabolites in the small intestine. For the qualitative and quantitative analyses, the HPLC-MS (High Performance Liquid Chromatography–Mass Spectrometry) and HPLC (High Performance Liquid Chromatography) techniques were used, respectively. Based on the obtained results, it was found that the best bioavailability of the selected ginger polyphenols (6-gingerol, 8-gingerdione, 8-shogaol, and 10-gingerdione) was estimated for a high-fiber diet, while the weakest results were obtained for standard and basic diets. In the case of the high-fiber diet, the bioavailability of the mentioned compounds was estimated as 33.3, 21.4, 6.73, and 21.0%, while for the basic diet, it was only 21.3, 5.3, 2.0, and 1.0%, respectively.

In Vitro Evaluation of Bioavailability of Cr from Daily Food Rations and Dietary Supplements from the Polish Market

“…The procedure used in the present study was improved and previously checked and validated for its accuracy in evaluating the bioavailability of various food components, including trace elements. All the details were described elsewhere [30,36].…”

Influence of Thermal Treatment on the Composition of Alpinia officinarum Rhizome

“…Extraction yield for all extracts was presented in Table S2 in the Supplementary Materials. For qualitative purposes, the samples were analyzed for their fingerprint and composition using the HPLC-ESI-QTOF-MS/MS platform according to previously described procedure with further modifications [110]. Prior to the analysis, the extracts were dissolved in methanol (at a concentration of 10 mg/mL) and then filtered through a nylon syringe filter with pore size of 0.1 µm.…”

“…The same extract solution (described above) was used for the quantitative study using HPLC analysis according to previously described procedure with further modifications [110]. During analysis solvent gradient elution mode was used.…”