Influence of dairy spent wash (DSW) on different cultivation phases and yield response of two Pleurotus mushrooms

Abstract

Two commercial oyster mushroom species, Pleurotus florida PF05 (PF) and Pleurotus sajor-caju PS08 (PS), were cultivated on wheat straw (WS), corn cob (CC) and combination of both (CC+WS) as basal substrates. These substrates were deliberately supplemented with 5, 10, 20 and 40% of dairy spent wash (DSW) as additive. The influence of DSW on cultivation phases of both mushrooms' lifecycle and other parameters such as mycelial growth rate, ergosterol production, mycelial run, pin head formation, yield, biological efficiency (BE) and protein content in fruitbodies was evaluated. Different DSW concentrations significantly (P< 0.05) exhibited continuous variations over different parameters. In contrast to the response of spent wash on PS, PF was superior in every phase and parameter. The lower concentrations up to a certain level unexpectedly influenced the cultivation phases of both mushrooms. The fastest growth rate (6.6 mm day −1 ) and highest ergosterol (437.00 μg g −1 ) were registered at 20% DSW with the combination of CC+WS as basal substrate. The earliest mycelial run and pin head formation by PF were recorded within 17 and 21 days at 10% supplementation of WS substrate. The maximum 2,173.62 g fruit body yield and 108.68% BE were obtained from PF at 10% DSW supplementation to CC+WS substrates. In addition, the highest content of protein (32.9 mg g −1 ) was achieved in the set of CC+WS with 20% DSW added. The results for each parameter of cultivation in supplemented sets were more enhanced compared to unsupplemented sets. The progression of yield response and biological efficiency was noted as flush-I>flush-II>flush-III with considerable differences. These results suggest the use of DSW at low levels for increasing fruit body yield and BE of Pleurotus mushrooms.

Differential Response of Oyster Shell Powder on Enzyme Profile and Nutritional Value of Oyster MushroomPleurotus floridaPF05

“…Similar level of protein content was also reported by other workers in P. florida and P. ostreatus mushrooms [2, 27]. Thus it can be speculated that availability of nutrients may affect the nutrient composition of fruit bodies [7]. Several kinds of amino acids are basically available in oyster shell [21] that may provide nutrients to fungus and might help in their protein synthesis.…”

Decolorization of synthetic brilliant green carpet industry dye through fungal co-culture technology

“…Fungal biomass and mycelial growth are known as quantitative characteristic of fungus (Naraian et al, 2011). We in the first experiment of the present study investigated response of coculture of two different species of fungi on production of fungal biomass.…”

Yield and nutritional composition of oyster mushroom strains newly introduced in Bangladesh

“…For successful cultivation, it is important to select high-yielding strains. However, the production and yield performance of commercial strains of mushrooms tend to decrease after consecutive subculturing (Naraian et al, 2011). Therefore, more information about this genus and its species is necessary to identify good strains to ensure continuous yield improvement (Uhart et al, 2008) and to screen the efficient varieties for Bangladesh.…”