Influence of culture conditions on the technological properties ofCarnobacterium maltaromaticumCNCM I‐3298 starters

Abstract

Aim The aim of this study is to investigate the effect of a broad spectrum of culture conditions on the acidification activity and viability of Carnobacterium maltaromaticum CNCM I‐3298, the main technological properties that determine the shelf‐life of biological time‐temperature integrator (TTI) labels. Methods and Results Cells were cultivated at different temperatures (20–37°C) and pH (6–9·5) according to a modified central composite design and harvested at increasing times up to 10 h of stationary phase. Acidification activity and viability of freeze‐thawed concentrates were assessed in medium mimicking the biological label. Acidification activity was influenced by all three culture conditions, but pH and harvest time were the most influential. Viability was not significantly affected by the tested range of culture conditions. Conclusions Carnobacterium maltaromaticum CNCM I‐3298 must be cultivated at 20°C, pH 6 and harvested at the beginning of stationary phase to exhibit fastest acidification activities. However, if slower acidification activities are pursued, the recommended culture conditions are 30°C, pH 9·5 and a harvest time between 4–6 h of stationary phase. Significance and Impact of the Study Quantifying the impact of fermentation temperature, pH and harvest time has led to a predictive model for the production of biological TTI covering a broad range of shelf‐lives.

Insights into lactic acid bacteria cryoresistance using FTIR microspectroscopy

“…The C. maltaromaticum strain was cultivated and frozen according to the procedure described by Girardeau et al [35], and the L. bulgaricus strains, according to the procedures described by Meneghel et al [2]. After centrifugation (Fig.…”

“…To determine a potential effect of the protective conditions applied that differed between L bulgaricus [2] and C. maltaromaticum [35], the biological activity recovery of C. maltaromaticum was also investigated for cell pellets re-suspended in a sucrose solution (20 % wt in saline water) at a weight ratio of 1:2 and 1:1 (cell pellet weight: protective solution weight).…”

“…The latter was included in this research because it is an extremely cryoresistant LAB reported to present no viability loss after 11 freeze-thaw cycles [34]. Moreover, C. maltaromaticum was shown to maintain its cryoresistance despite changes in growth conditions (temperature, pH, and harvest time) [35]. The membrane fatty acid composition, lipid membrane phase transition following freezing, membrane fluidity following cooling, and intracellular glass transition temperature were also measured to extend the range of markers of cryoresistance.…”

Lactic acid production by Carnobacterium sp. isolated from a maritime Antarctic lake using eucalyptus enzymatic hydrolysate

“…For instance, it has been reported that high levels of some elements such as Mn 2+ , can improve biomass growth for some Carnobacterium spp. [ 43 , 44 ]. Acetic acid production was only observed at the initial batch fermentation (2.3 g/L), while no acetic acid production was observed after the pulse, probably due to the poor consumption of the remaining xylose.…”

Dynamic Modeling of Carnobacterium maltaromaticum CNCM I-3298 Growth and Metabolite Production and Model-Based Process Optimization

“…Data used to calibrate and validate the model were partially reported in a previous study, in which a modified central composite experimental design was carried out to study the effect of operating conditions on the technological properties of C. maltaromaticum CNCM I-3298 [7]. Sixteen lab-scale fermentations (hereafter named F01 to F16) were performed using a wide range of regulated operating conditions (Figure 2): temperature between 20 and 37 • C and pH between 6.0 and 9.5. and trehalose, with similar growth rates.…”