Influence of copper ions on the viability and cytotoxicity of Pseudomonas aeruginosa under conditions relevant to drinking water environments

Dwidjosiswojo, Zenyta; Richard, Jessica; Moritz, Miriam M.; Dopp, Elke; Flemming, Hans‐Curt; Wingender, Jost

doi:10.1016/j.ijheh.2011.06.004

Cited by 84 publications

(88 citation statements)

References 27 publications

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“…33 Copper concentrations observed in drinking water would therefore affect culturability without affecting viability, with the capacity to recover culturability once copper stress is reduced. 22 Mean residual chlorine (Fig. 1b) was slightly more elevated in negative samples and was a significant variable for aerator positivity according to qPCR and water P. aeruginosa load (Table 4).…”

Section: Discussionmentioning

confidence: 95%

“…In this study, mean copper concentration in water was higher (570 μg/L) than the concentration reported to inhibit P. aeruginosa culturability (250 μg/L). 21,22 However, this threshold concentration was obtained using strains not previously acclimated to elevated copper concentrations. Viable but nonculturable P. aeruginosa cells can persist in the presence of copper concentrations up to 2 mM, which is orders of magnitude higher than copper concentrations found in drinking water.…”

Section: Discussionmentioning

confidence: 98%

“…Furthermore, bacteria present in the water distribution system are exposed to stressors such as chlorine, copper, and high temperatures. 21,22 In drinking water, stressed cells that lose their culturability can be detected by qPCR; these cells can quickly recover their culturability under favorable conditions. 21 These findings suggest the need to re-evaluate the prevalence of P. aeruginosa in the water system using a standardized sample volume for culture and the need to apply qPCR methods recently validated in drinking water.…”

Section: Discussionmentioning

confidence: 99%

“…Environmental stressors present in water such as chlorine and copper decrease culturability without necessarily decreasing viability. 21,22 Most studies describing environmental contamination have been conducted using the culture detection method. Often, P. aeruginosa could not be isolated from water but was recovered from biofilm swabs.…”

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confidence: 99%

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Post-Outbreak Investigation of Pseudomonas aeruginosa Faucet Contamination by Quantitative Polymerase Chain Reaction and Environmental Factors Affecting Positivity

Bédard

Laferrière

Charron

et al. 2015

Infect. Control Hosp. Epidemiol.

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OBJECTIVETo perform a post-outbreak prospective study of the Pseudomonas aeruginosa contamination at the faucets (water, aerator and drain) by culture and quantitative polymerase chain reaction (qPCR) and to assess environmental factors influencing occurrenceSETTINGA 450-bed pediatric university hospital in Montreal, CanadaMETHODSWater, aerator swab, and drain swab samples were collected from faucets and analyzed by culture and qPCR for the post-outbreak investigation. Water microbial and physicochemical parameters were measured, and a detailed characterization of the sink environmental and design parameters was performed.RESULTSThe outbreak genotyping investigation identified drains and aerators as the source of infection. The implementation of corrective measures was effective, but post-outbreak sampling using qPCR revealed 50% positivity for P. aeruginosa remaining in the water compared with 7% by culture. P. aeruginosa was recovered in the water, the aerator, and the drain in 21% of sinks. Drain alignment vs the faucet and water microbial quality were significant factors associated with water positivity, whereas P. aeruginosa load in the water was an average of 2 log higher for faucets with a positive aerator.CONCLUSIONSP. aeruginosa contamination in various components of sink environments was still detected several years after the resolution of an outbreak in a pediatric university hospital. Although contamination is often not detectable in water samples by culture, P. aeruginosa is present and can recover its culturability under favorable conditions. The importance of having clear maintenance protocols for water systems, including the drainage components, is highlighted.Infect. Control Hosp. Epidemiol. 2015;36(11):1283–1291

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Post-Outbreak Investigation of Pseudomonas aeruginosa Faucet Contamination by Quantitative Polymerase Chain Reaction and Environmental Factors Affecting Positivity

Bédard

Laferrière

Charron

et al. 2015

Infect. Control Hosp. Epidemiol.

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“…The inability to grow viable but non-culturable (VBNC) P. aeruginosa due to heavy metals 588 (Dwidjosiswojo et al 2011) or chlorine in wastewater would mean that it is technically 589 challenging to provide a precise concentration of P. aeruginosa and hence an accurate 590 determination of the microbial risks. Alternatively, qPCR can be used to determine the copy 591 numbers of P. aeruginosa in the water samples.…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Removal of bacterial contaminants and antibiotic resistance genes by conventional wastewater treatment processes in Saudi Arabia: Is the treated wastewater safe to reuse for agricultural irrigation?

et al. 2015

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Please cite this article as: Al-Jassim, N., Ansari, M.I., Harb, M., Hong, P.-Y., Removal of bacterial contaminants and antibiotic resistance genes by conventional wastewater treatment processes in Saudi Arabia: Is the treated wastewater safe to reuse for agricultural irrigation?, Water Research (2015Research ( ), doi: 10.1016Research ( /j.watres.2015 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT Abstract 1This study aims to assess the removal efficiency of microbial contaminants in a local wastewater 2 treatment plant over the duration of one year, and to assess the microbial risk associated with 3 reusing treated wastewater in agricultural irrigation. The treatment process achieved 3.9 logs 4 removal of heterotrophic bacteria and up to 3.9 logs removal of fecal coliforms. The final 5 chlorinated effluent had 1.8 x 10 2 MPN/100 mL of fecal coliforms and fulfils the required quality 6 for restricted irrigation. 16S rRNA gene-based high-throughput sequencing showed that several 7 genera associated with opportunistic pathogens (e.g. Acinetobacter, Aeromonas, Arcobacter, 8 Legionella, Mycobacterium, Neisseria, Pseudomonas and Streptococcus) were detected at 9 relative abundance ranging from 0.010 to 21 % of the total microbial community in the influent. 10

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Antimicrobial Surfaces

Gratzl

Paulik

Lackner

2014

Kirk-Othmer Encyclopedia of Chemical Technology

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Biofouling, the attachment of biological material onto surfaces, is a pervasive and costly problem affecting marine equipment, membrane‐based systems, medical devices, textiles, food packing, and other systems and devices. The attachment of biological material to surfaces is often attributed to an initial microbial settling and the subsequent formation of a microbial biofilm on a surface. Antimicrobial agents have been used to prevent or reduce biofilm formation and are effective if their concentration is sufficiently high; however, biocides are toxic substances that affect the surrounding environment. This article provides an overview of the most important antimicrobial agents, technologies, and recent developments aimed at preventing biofouling. It focuses on antimicrobial surfaces and their current and prospective use, proposed mechanisms of action, and applications.

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Influence of copper ions on the viability and cytotoxicity of Pseudomonas aeruginosa under conditions relevant to drinking water environments

Cited by 84 publications

References 27 publications

Post-Outbreak Investigation of Pseudomonas aeruginosa Faucet Contamination by Quantitative Polymerase Chain Reaction and Environmental Factors Affecting Positivity

Post-Outbreak Investigation of Pseudomonas aeruginosa Faucet Contamination by Quantitative Polymerase Chain Reaction and Environmental Factors Affecting Positivity

Removal of bacterial contaminants and antibiotic resistance genes by conventional wastewater treatment processes in Saudi Arabia: Is the treated wastewater safe to reuse for agricultural irrigation?

Antimicrobial Surfaces

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