Influence of copper (I) nicotinate complex and autophagy modulation on doxorubicin-induced cytotoxicity in HCC1806 breast cancer cells

Abdel‐Mohsen, Mohamed A.; Malak, Camelia A. Abdel; El‐Shafey, Eman Salah

doi:10.1016/j.advms.2018.08.014

Cited by 23 publications

(12 citation statements)

References 45 publications

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“…Real‐time was performed using the BeyoRT™ II First Strand cDNA Synthesis Kit (Beyotime Institute of Biotechnology) and then qPCR was performed using the Light‑Cycler 480 System (Roche Diagnostics), with SYBR Green reagent (Takara Biotechnology, Co., Ltd.). The amplification reactions were according to the following thermal cycling conditions: 95℃ for 10 min, 40 cycles of 95˚C for 15 s, and 60˚C for 30 s. The quantification was expressed as the ratio of target genes to GAPDH mRNA using the 2 −ΔΔCq method (Abdel‐Mohsen et al., 2017; Abdel‐Mohsen et al, 2019a, 2019b). reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was accomplished by the following primers:…”

Section: Methodsmentioning

confidence: 99%

“…The Notch signaling pathway is a well‐preserved molecular mechanism that coordinates cellular events that occur within a neighboring cell community (Abdel‐Mohsen et al, 2019a, 2019b). The interaction among ligands and receptors of the Notch pathway is critical in deciding the status of Notch and the impact of any particular cellular event often induced by other signaling pathways (Bigas & Espinosa, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Ziziphus spina‐christi (L.) fortified with Camellia sinensis mediates apoptosis, Notch‐1 signaling, and mitigates obesity‐induced non‐alcoholic fatty liver

2021

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The habit of drinking tea is highly prevalent and could be utilized to introduce more health benefits through fortification with medicinal plants. The purpose of this analysis was to assess the nutritional quality and health benefits of fortified Ziziphus tea (ZT) with green tea (GT) against obesity and non‐alcoholic fatty liver disease (NAFLD). Proximate analysis and sensory evaluation were carried out on the fortified tea. In the in vivo study, 15 SD rats were used for each group. Flow cytometry was utilized for caspase 3 analysis. ELISA was used for the detection of tumor necrosis factor‐alpha and adiponectin levels. Real‐time PCR was used to detect Notch‐1 and Hes‐1 gene expression. The composition of fortified (GT+ZT) showed a significant improvement in the nutritional value represented by the increase in overall protein, crude fat, crude fiber, ash, carbohydrate, mineral contents, and antioxidant capacity. Treatment with GT+ZT restored the disturbance in body weight, lipid profile, liver function, glucose, insulin sensitivity index, and oxidative status. It reversed the changes in TNF‐α and adiponectin levels. Their protective effects against NAFLD were indicated by the inhibition of hepatic caspase‐3 activity, suppression of Notch‐1, and Hes‐1 gene expression and amelioration of high‐fat diet (HFD)‐induced histological alterations. Collectively, our findings, elucidate the precise mechanism of fortified ZT+GT for the attenuation of obesity‐induced metabolic disorders and NAFLD via regulating lipolysis, TNF‐α, adiponectin, apoptosis, and Notch‐1 signaling pathways, and provide a foundation for an easily implemented healthy habit of drinking. Practical applications The incorporation of bioactive compounds into functional foods is a growing market. Consumer attention in well‐being has increased rapidly toward a fortified diet that provides additional health effects. The fortified (GT+ZT) tea may potentially serve as an easily implemented healthy drinking habit to prevent and manage obesity and NAFLD and reduce the risk of other diseases. Fortification with ZT improved the health‐promoting functionality of GT through the enhancement of total protein, carbohydrates, antioxidant, and mineral contents. This was reflected by their synergetic therapeutic activity in ameliorating the disturbance in obesity‐related disorders and NAFLD via regulating lipolysis, inflammation, oxidative stress, apoptosis, and Notch‐1 signaling pathways. Therefore, (GT+ZT) could be considered functional foods which attribute to functional improvement and reduction in disease risk.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ziziphus spina‐christi (L.) fortified with Camellia sinensis mediates apoptosis, Notch‐1 signaling, and mitigates obesity‐induced non‐alcoholic fatty liver

2021

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“…Casiopeina III‐ia (Trejo‐Solis et al, 2012), a Cu complex, induced autophagy in glioma cells via reactive oxygen species (ROS) and JNK. Moreover, Cu(I) nicotinate complex (CNC) plays an inhibitory role in autophagy induced by Torin (Abdel‐Mohsen et al, 2017). The transcription factor CRR1, a global regulator of Cu signaling in Chlamydomonas , is involved in the Cu‐induced expression of autophagy‐related genes (Perez‐Martin et al, 2015).…”

Section: Effects Of Transition Metals On Autophagymentioning

confidence: 99%

Transition metals and metal complexes in autophagy and diseases

Luo

Huang

et al. 2021

Journal Cellular Physiology

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Transition metals refer to the elements in the d and ds blocks of the periodic table. Since the success of cisplatin and auranofin, transition metal‐based compounds have become a prospective source for drug development, particularly in cancer treatment. In recent years, extensive studies have shown that numerous transition metal‐based compounds could modulate autophagy, promising a new therapeutic strategy for metal‐related diseases and the design of metal‐based agents. Copper, zinc, and manganese, which are common components in physiological pathways, play important roles in the progression of cancer, neurodegenerative diseases, and cardiovascular diseases. Furthermore, enrichment of copper, zinc, or manganese can regulate autophagy. Thus, we summarized the current advances in elucidating the mechanisms of some metals/metal‐based compounds and their functions in autophagy regulation, which is conducive to explore the intricate roles of autophagy and exploit novel therapeutic drugs for human diseases.

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“…Ice‐cold methanol (100%), was applied slowly to allow cellular permeabilization. Cells were vortexed gently to a final concentration of methanol (90%), followed by analysis using the appropriate filter (Abdel‐Mohsen, Abdel Malak, et al, 2019; Murugan and Amaravadi, 2016).…”

Section: Methodsmentioning

confidence: 99%

Fortification of biscuit with sidr leaf and flaxseed mitigates immunosuppression and nephrotoxicity induced by cyclosporine A

2021

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The focus of consumers in healthy food turned to the possible health benefits of particular foods and food ingredients. This study aimed to evaluate the newly fortified biscuits supplemented with sidr leaves and flaxseed and to highlight their nutritional quality and health benefits against cyclosporine A-induced dexterous effects.Sidr leaves (SL), and flaxseed (FS) were used in the preparation of fortified biscuits.Proximate analysis and sensory evaluation were carried out on the biscuits. In in vivo study, 15 male albino mice were used for each group. Groups were divided into control, CsA, SL, FS, and SL+FS-treated groups. Hematological analysis, kidney function tests, oxidative stress, and anti-oxidant status were estimated. Flow cytometry was utilized to detect apoptosis and autophagy levels. The enzyme-linked immunosorbent assay (ELISA) was used for detection of interleukin-2 (IL-2), interferonγ (IFNγ), and transforming growth factor β1 (TGF-β1) levels. The composition of biscuits complemented by SL and FS demonstrated significant improvement in the nutritional value represented by the increase in overall protein, crude fat, crude fiber, ash, and carbohydrate contents. Treatment with SL and FS restored the disturbance in hematological, kidney function, oxidative, and antioxidant biomarkers. CsA-induced apoptotic and autophagic renal cell death was suppressed. Cytokines and pro-inflammatory markers were ameliorated. The use of SL and FS in dietary products can be recommended as a functional food. Moreover, they showed renal-protective, antioxidant, anti-inflammatory, and immune-enhancing activities. Practical applicationsSidr leaves (SL) and flaxseed (FS) were used in the preparation of fortified biscuits.The composition of biscuits complemented by SL and FS demonstrated a significant improvement in the nutritional values represented by the increase in overall protein, crude fat, crude fiber, ash, and carbohydrate contents. SL and FS showed a potential therapeutic activity in reversing CsA-induced dexterous side effects by acting as an antioxidant, antiapoptotic, antiautophagic, anti-inflammatory, renal-protective, and How to cite this article: El Seedy GM, El-Shafey ES, Elsherbiny ES. Fortification of biscuit with sidr leaf and flaxseed mitigates immunosuppression and nephrotoxicity induced by cyclosporine A.

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Influence of copper (I) nicotinate complex and autophagy modulation on doxorubicin-induced cytotoxicity in HCC1806 breast cancer cells

Cited by 23 publications

References 45 publications

Ziziphus spina‐christi (L.) fortified with Camellia sinensis mediates apoptosis, Notch‐1 signaling, and mitigates obesity‐induced non‐alcoholic fatty liver

Ziziphus spina‐christi (L.) fortified with Camellia sinensis mediates apoptosis, Notch‐1 signaling, and mitigates obesity‐induced non‐alcoholic fatty liver

Transition metals and metal complexes in autophagy and diseases

Fortification of biscuit with sidr leaf and flaxseed mitigates immunosuppression and nephrotoxicity induced by cyclosporine A

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