Influence of controlled hypoxia and radical scavenging agents on erythropoietin and malondialdehyde concentrations in humans

Freudenthaler, Stefan M.; Schreeb, K H; Wiese, Anne; Pilz, Jürgen; Gleiter, Christoph H.

doi:10.1046/j.1365-201x.2002.00946.x

Cited by 32 publications

(22 citation statements)

References 27 publications

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“…10 The end products of the reactions between ROS and polyunsaturated fatty acids are hydroperoxides, and malondialdehyde is one of the major aldehydes produced by the breakdown of such hydroperoxides. The hypoxia-reoxygenation cycles in OSAS were suggested to cause increases in malondialdehyde levels, which was also confirmed by the studies that showed increased malondialdehyde levels following hypoxemia in healthy controls, 40 or hyperoxic reoxygenation in surgical subjects. 41 Our results also confirm these findings.…”

Section: Discussionsupporting

confidence: 66%

Evaluation of Oxidative Damage and Antioxidant Mechanisms in COPD, Lung Cancer, and Obstructive Sleep Apnea Syndrome

et al. 2015

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BACKGROUND: Oxidative damage is a major contributing factor to carcinogenesis and obstructive disorders in lungs. Current evidence suggests that the inflammatory processes yield to oxidative mechanisms, which underlie COPD, lung cancer, and obstructive sleep apnea syndrome (OSAS). This study aimed to evaluate the oxidative damage in these diseases by evaluating the oxidative and antioxidant biomarkers. METHODS: Malondialdehyde, 8-oxo-7,8-dihydro-2-deoxyguanosine, and coenzyme Q10 levels were evaluated in the blood samples of subjects with COPD, lung cancer, and OSAS by high-pressure liquid chromatography. RESULTS: A total of 111 participants (35 females, 76 males) with OSAS (n ‫؍‬ 29), COPD (n ‫؍‬ 26), and lung cancer (n ‫؍‬ 28) and healthy controls (n ‫؍‬ 28) were included in the study. The malondialdehyde and coenzyme Q10 levels were significantly higher in all 3 diseases when compared with controls (P < .01), whereas 8-oxo-7,8-dihydro-2-deoxyguanosine levels were only significantly higher than in healthy controls in subjects with lung cancer (P ‫؍‬ .005). The highest levels of malondialdehyde and coenzyme Q10 were determined in subjects with OSAS and lung cancer, respectively. The highest 8-oxo-7,8-dihydro-2-deoxyguanosine levels were also observed in subjects with lung cancer, but the differences of this biomarker with other diagnoses were not statistically significant (P ‫؍‬ .56). CONCLUSION: Oxidative damage was observed in all 3 diagnoses, and, as a response to oxidative stress, antioxidant mechanisms were also active in these diseases. Malondialdehyde and 8-oxo-7,8-dihydro-2-deoxyguanosine were found to be efficiently usable in the evaluation of oxidative damage in chronic respiratory diseases. (ClinicalTrials.gov registration NCT02406053.) Key words: malondialdehyde; 8-oxo-7,8-dihydro-2Ј-deoxyguanosine;coenzyme Q10; COPD; OSAS; lung cancer; oxidative damage; antioxidants. [Respir Care 2016;61(2):205-211.

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Section: Discussionsupporting

confidence: 66%

Evaluation of Oxidative Damage and Antioxidant Mechanisms in COPD, Lung Cancer, and Obstructive Sleep Apnea Syndrome

et al. 2015

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“…The order of magnitude of the increase of Epo concentrations in humans under i.v. AII administration is very similar to that of hypoxia (exposure to 13% O 2 or altitude levels of about 4000 m) [5,23,24].…”

Section: Discussionmentioning

confidence: 70%

Do alterations of endogenous angiotensin II levels regulate erythropoietin production in humans?

Freudenthaler¹,

Benöhr

Grenz

et al. 2003

Brit J Clinical Pharma

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Aims Recent evidence suggests a potential role of angiotensin II in the physiological regulation of erythropoietin (Epo) production. While the administration of exogenous angiotensin II (AII) has been used so far to study its effects, the role of endogenous AII has remained unclear. Methods To alter endogenous AII in humans experimentally we used furosemide bolus injection as a short-term (study 1) and dietary salt as a long-term modulator (study 2). In an open crossover design, 12 healthy male volunteers received furosemide (F) 0.5 mg kg -1 intravenously or placebo (P) in random order (study 1). With the same design, 12 volunteers received high-salt (HS), normal-salt (NS) and lowsalt (LS) diet (study 2). Plasma renin activity (PRA) was analysed along with AII. Inulin and paraaminohippurate (PAH) clearances were used to indicate glomerular filtration rate (GFR) and renal plasma flow (RPF), respectively. Results While F stimulated AII and PRA and decreased GFR and RPF significantly, no concomitant alteration of Epo was observed [AUCEpo: placebo 5709 ± 243 (% of baseline ¥ h), furosemide: 5833 ± 255 (% of baseline ¥ h); 95% confidence interval (CI) -608.4, 856.0; P = 0.73]. F decreased GFR (from 103.6 ± 4.0 to 90.6 ± 4.8 ml min -1 1 -1 73 m -2 ; 95% CI 1.1, 24.9; P < 0.05), but not RPF (study 1). Correspondingly, LS stimulated and HS decreased AII and PRA significantly. HS increased GFR and RPF. Again, Epo concentrations were not affected (AUCEpo: normal sodium 44 ± 6.7 mIU ¥ day ml -1 , low sodium 39 ± 2.4 mIU ¥ day ml -1 , high sodium 48.5 ± 6.1 mIU ¥ day ml -1 ; normal salt/low salt 95% CI -11.9, 21.9, P = 0.54; normal salt/high salt 95% CI -14.4, 23.3, P = 0.63; study 2). Conclusions We conclude that, at least in the physiological setting in healthy volunteers, increased concentrations of endogenous AII may not be a major factor of Epo regulation.

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“…Freudenthaler S et al increased concentrations of endogenous angiotensin II and PRA and decreased GFR and RPF significantly, with no concomitant alteration of Epo regulation in humans [17]. Reversely, the majority of the following examples concern [20]. Gargano G et al improved epoetin-α or r-HuEPO SC administration by sodium ferrous gluconate administered IV along [21], [22].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Erythropoietin on Sodium During Ischemia Reperfusion Injury in Rats

Tsompos¹

2015

Ann Clin Anal Med

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Influence of controlled hypoxia and radical scavenging agents on erythropoietin and malondialdehyde concentrations in humans

Abstract: Taken together, our in-vivo results do not support a gross modulatory effect of a short-term treatment with radical scavenging agents on EPO-production during or after hypoxia in humans, as derived from the detected changes of MDA-concentrations in peripheral plasma.

Cited by 32 publications

References 27 publications

Evaluation of Oxidative Damage and Antioxidant Mechanisms in COPD, Lung Cancer, and Obstructive Sleep Apnea Syndrome

Evaluation of Oxidative Damage and Antioxidant Mechanisms in COPD, Lung Cancer, and Obstructive Sleep Apnea Syndrome

Do alterations of endogenous angiotensin II levels regulate erythropoietin production in humans?

The Effect of Erythropoietin on Sodium During Ischemia Reperfusion Injury in Rats

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