Influence of climate variation on phenolic composition and antioxidant capacity of Medicago minima populations

Kabtni, Souhir; Sdouga, Dorra; Rebey, Ines Bettaib; Save, Mattew; Trifi-Farah, Neïla; Fauconnier, Marie‐Laure; Marghali, Sonia

doi:10.1038/s41598-020-65160-4

Cited by 70 publications

(49 citation statements)

References 64 publications

(171 reference statements)

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“…The phytochemical content and pharmacological effects of a medicinal plant species will differ depending on the geographical conditions where the plant grows 25 , 26 . Therefore, to select medicinal plants that have superior performance for the plant breeding programs, limited environmental conditions are needed 27 , 28 .…”

Section: Discussionmentioning

confidence: 99%

Genotype selection for phytochemical content and pharmacological activities in ethanol extracts of fifteen types of Orthosiphon aristatus (Blume) Miq. leaves using chemometric analysis

Batubara

Komariah

Sandrawati

et al. 2020

Sci Rep

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Orthosiphon aristatus (Blume) Miq. of the Lamiaceae family, called as kumis kucing in Indonesia, is a valuable medicinal plant for their pharmacological properties. The present study comprised of fifteen genotypes of O. aristatus was undertaken to evaluate the genotypes based on phytochemical content and pharmacological activities of leaves ethanol extract. Chemometric analysis (correlation and principal component analysis) was also used to investigate the genetic variability based on phytochemical content and pharmacological activities of O. aristatus genotypes. Results of phytochemical characterization showed that total phenolic ranged from 1.48 to 36.08 (maximum in A15) mg GAE/g DW, total flavonoid ranged from 0.10 to 3.07 (maximum in A15) mg QE/g DW, sinensetin ranged from 0.36 to 4.02 (maximum in A11) mg/g DW, and rosmarinic acid ranged 0.06 to 7.25 (maximum in A7) mg/g DW. Antioxidant activity was tested using DPPH and FRAP assay. Antioxidant results showed that DPPH ranged from 1.68 to 15.55 (maximum in A15) μmol TE/g DW and FRAP ranged from 0.07 to 1.60 (maximum in A1 and A7) μmol TE/g DW. The genotype A8 showed the highest cytotoxic activities against HeLa (66.25%) and MCF-7 (61.79%) cell lines. Maximum α-glucosidase inhibitory activity was recorded in genotype A2 with the value of 62.84%. The genotypes A1, A2, A7, A11, and A15 were identified as superior based on their phytochemicals content and pharmacological activities coupled with chemometric analysis. This finding is important for breeding studies and also the pharmaceutical perspective of O. aristatus.

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Section: Discussionmentioning

confidence: 99%

Genotype selection for phytochemical content and pharmacological activities in ethanol extracts of fifteen types of Orthosiphon aristatus (Blume) Miq. leaves using chemometric analysis

Batubara

Komariah

Sandrawati

et al. 2020

Sci Rep

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“…The obtained UAE extract showed higher DPPH and FRAP values and significantly higher content of redox-active compounds (TPC, TFC, and TAC). This fact is not surprising considering the reports in the literature about the positive correlation between the content of redox-active compounds—particularly the phenols and anthocyanins of berry extracts—and DPPH and FRAP in vitro assays [ 45 ]. The DPPH IC50 values of samples in this work were higher than those reported in enzymatically treated anthocyanin fractions of the raspberry pomace [ 54 ].…”

Section: Resultsmentioning

confidence: 86%

“…The phenolic content can vary even among specimens of the same plant species, and the results of TPC are influenced by environmental factors such as climate, coastal proximity, soil, etc. Some plant species found in colder climates, at higher altitudes, and growing in a more arid environment tend to produce more phenols than the same species growing under different conditions [ 45 , 46 ]. The studied raspberry ( Rubus idaeus L.) tends to fare better in colder climates and higher altitude and this probably accounts for the relatively high TPC found in the pomace samples analyzed.…”

Section: Resultsmentioning

confidence: 99%

Biowaste as a Potential Source of Bioactive Compounds—A Case Study of Raspberry Fruit Pomace

Krivokapić

Vlaović

Vratnica

et al. 2021

Foods

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Raspberry fruit pomace, a byproduct of juice production, was studied as a potential source of antioxidant compounds. Target high-performance liquid chromatography analysis of important polyphenolic compounds (gallic, p-coumaric, caffeic, quercitrin, chlorogenic, and ellagic acid) was performed together with analysis of total phenolic content (TPC), total flavonoid content (TFC), total anthocyanins content (TAC), and antioxidant capacity (via ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays). The differences in polyphenolic content of Rubus idaeus L. pomace were evaluated following ultrasound-assisted extraction and conventional maceration with different organic solvents. Additionally, the yield of free phenolics was measured in hydrolyzed pomace extracts. The results obtained show that the ultrasound method maximizes the quantity of antioxidant compounds in terms of TPC (27.79 mg/L gallic acid equivalent (GAE)), TFC (8.02 mg/g quercetin equivalent (QE)), TAC (7.13 mg/L cyanidine-3-glucoside equivalent (C3G Eq)), caffeic (19.17 µg/g), chlorogenic (3.56 µg/g), ellagic (105.52 µg/g), and gallic acids (8.75 µg/g), as well as FRAP (1002.72 µmol/L) and DPPH assays (969.71 µmol/mL vitamin C equivalent (vit C Eq); 567.00 µmol/100 g Trolox equivalent (TE)). On the other hand, conventional maceration maximizes the yield of quercetin and p-coumaric acid. In terms of biowaste valorization, raspberry fruit pomace has a promising industrial potential and may prove to be useful in the development of antioxidant dietary supplements.

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“…However, huge variation is noted in the literature for the phenolics content, and antioxidant activities measured in Chaga. The variation in antioxidant activities and phenolics content reported in the literature are reported to be influenced by source geography, temperature, carrier birch type, time of harvest, and part used for extraction [27].…”

Section: Total Polyphenols Content (Tpc) and Antioxidant Activity (Aa) Of Myconutrients From Chaga Extractmentioning

confidence: 99%

The Influence of pH and Temperature on Water as a Pressurized Fluid in the Extraction of Bioactive Myconutrients from Chaga Mushroom (<em>Inonotus obliquus</em>)

Im¹,

Critch²,

Manful³

et al. 2021

Preprint

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Mushrooms have long rich history in folk medicine, traditional and functional foods due to high content of dietary myco-nutrients. Currently, there is increased interest in finding appropriate food-grade green ex-traction systems capable of extracting these bioactive compounds from dietary mushrooms for applica-tions in various food, pharmacological or nutraceutical formulations. Herein, we evaluated a modified Swiss water process (SWP) method using alkaline and acidic pH at low and high temperature under pressurized conditions as a suitable green food grade solvent to obtained extracts enriched with my-co-nutrients (dietary phenolics, total antioxidants (TAA), vitamins, and minerals) from Chaga. Ultra-high performance liquid chromatography coupled to high resolution accurate mass tandem mass spectrometry (UHPLC-HRAMS-MS/MS) was used to assess the phenolic compounds and vitamin levels in the extracts, while inductively coupled plasma mass spectrometry (ICP-MS) was used to determine the mineral con-tents. Over twenty phenolic compounds were quantitatively evaluated in the extracts and the highest total phenolic content and antioxidant activity was observed at pH11.5 at 100°C. The most abundant phenolic compounds present in Chaga extracts included phenolic acids such as protocatechuic acid 4-glucoside (0.7-1.08µg/mL), syringic acid (0.62-1.18µg/mL), and myricetin (0.68-1.3µg/mL). Vitamins are being reported for the first time in Chaga. pH 2.5 at 100°C treatment shows superior effects in extracting the B vitamins whereas pH 2.5 at 60 and 100°C treatments were outstanding for extraction of total fat-soluble vitamins. Vitamin E content was the highest for the fat-soluble vitamins in the Chaga extract under acidic pH (2.5) and high temp. (100°C) and ranges between 50 to 175 µg/100g Chaga. Antioxidant minerals ranged from 85.94 µg/g (pH7 at 100°C) to 113.86 µg/g DW (pH2.5 at 100°C). High temperature 100°C and a pH of 2.5 or 9.5. The treatment of pH11.5 at 100°C was the most useful for recovering phenolics and antioxidants from Chaga including several phenolic compounds reported for the first time in Chaga. SWP is being proposed herein for the first time as a novel, green food-grade solvent system for the extraction of myco-nutrients from Chaga and have potential applications as a suitable approach to extract nutrients from other matrices. Chaga extracts enriched with bioactive myconutrients and antioxidants may be suitable for further use or applications in the food and nutraceutical industries.

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Influence of climate variation on phenolic composition and antioxidant capacity of Medicago minima populations

Cited by 70 publications

References 64 publications

Genotype selection for phytochemical content and pharmacological activities in ethanol extracts of fifteen types of Orthosiphon aristatus (Blume) Miq. leaves using chemometric analysis

Genotype selection for phytochemical content and pharmacological activities in ethanol extracts of fifteen types of Orthosiphon aristatus (Blume) Miq. leaves using chemometric analysis

Biowaste as a Potential Source of Bioactive Compounds—A Case Study of Raspberry Fruit Pomace

The Influence of pH and Temperature on Water as a Pressurized Fluid in the Extraction of Bioactive Myconutrients from Chaga Mushroom (<em>Inonotus obliquus</em>)

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