Objectives:
To measure and compare
in vivo
and
in vitro
pulp temperature (PT) increase (ΔTEMP) over baseline, physiologic temperature using the same intact upper premolars exposed to the same Polywave
®
LED curing light.
Methodology:
After local Ethics Committee approval (#255,945), local anesthesia, rubber dam isolation, small occlusal preparations/minute pulp exposure (n=15) were performed in teeth requiring extraction for orthodontic reasons. A sterile probe of a temperature measurement system (Temperature Data Acquisition, Physitemp) was placed within the pulp chamber and the buccal surface was sequentially exposed to a LED LCU (Bluephase 20i, Ivoclar Vivadent) using the following exposure modes: 10-s low or high, 5-s Turbo, and 60-s high. Afterwards, the teeth were extracted and K-type thermocouples were placed within the pulp chamber through the original access. The teeth were attached to an assembly simulating the
in vivo
environment, being similarly exposed while real-time temperature (°C) was recorded. ΔTEMP values and time for temperature to reach maximum (ΔTIME) were subjected to two-way ANOVA and Bonferroni's
post-hoc
tests (pre-set alpha 0.05).
Results:
Higher ΔTEMP was observed
in vitro
than
in vivo
. No significant difference in ΔTIME was observed between test conditions. A significant, positive relationship was observed between radiant exposure and ΔTEMP for both conditions (
in vivo
: r2=0.917; p<0.001;
in vitro
: r2=0.919; p<0.001).
Conclusion:
Although the
in vitro
model overestimated
in vivo
PT increase,
in vitro
PT rise was close to
in vivo
values for clinically relevant exposure modes.