Influence of Cd²⁺on the spin state of non-heme iron and on protein local motions in reactions centers from purple photosynthetic bacteriumRhodospirilium rubrum

Chemical proprieties of the iron-quinone complex in mutated reaction centers of Rb. sphaeroides

“…Its hyperfine parameters do not change with the increasing temperature. A similar NHFe state was already observed in other photosynthetic systems of type II [4][5][6][7]. To understand the complexity of the AATyr spectra we performed measurements of the amount of ubiquinone binding at the Q B site per reaction center using HPLC [14].…”

Chemical proprieties of the iron-quinone complex in mutated reaction centers of Rb. sphaeroides

“…Its hyperfine parameters do not change with the increasing temperature. A similar NHFe state was already observed in other photosynthetic systems of type II [4][5][6][7]. To understand the complexity of the AATyr spectra we performed measurements of the amount of ubiquinone binding at the Q B site per reaction center using HPLC [14].…”

“…sphaeroides and of Rp. rubrum [4,5] and an exclusively low spin state in photosystem II of algae PSI − mutant [6,7]. We focused on studies of the NHFe valence and spin states in BRCs isolated form a wild type (WT) and two mutants of Rb.…”

Quantification of purple non-sulphur phototrophic bacteria and their photosynthetic structures by means of total reflection X-ray fluorescence spectrometry (TXRF)

“…One should also expect to conrm the presence of Mo but, due to the use of a Mo X-ray tube as an excitation source, the detection of Mo or Cd was not possible. However, it has been shown that Cd has a high affinity binding site in the vicinity of the non-haem iron in RCs, 51,52 hence its presence in the culturing medium would result in its accumulation in the system investigated, in particular in anaerobically grown cells and chromatophores. The elements determined in Rb.…”