Quantification of purple non-sulphur phototrophic bacteria and their photosynthetic structures by means of total reflection X-ray fluorescence spectrometry (TXRF)

Fiedor, Joanna; Ostachowicz, Beata; Baster, Monika; Lankosz, M.; Burda, Květoslava

doi:10.1039/c6ja00207b

Cited by 7 publications

(6 citation statements)

References 50 publications

(53 reference statements)

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“…Samples were processed as described by Latorre et al (2015). Samples were measured with a S2 PICOFOX TM TXRF spectrometer (Bruker, Germany) (González et al, 1999; Fiedor et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

The Combined Effect of Cold and Copper Stresses on the Proliferation and Transcriptional Response of Listeria monocytogenes

Quesille-Villalobos¹,

Parra²,

Maza³

et al. 2019

Front. Microbiol.

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Listeria monocytogenes is a foodborne pathogen that can cause severe disease in susceptible humans. This microorganism has the ability to adapt to hostile environmental conditions such as the low temperatures used by the food industry for controlling microorganisms. Bacteria are able to adjust their transcriptional response to adapt to stressful conditions in order to maintain cell homeostasis. Understanding the transcriptional response of L. monocytogenes to stressing conditions could be relevant to develop new strategies to control the pathogen. A possible alternative for controlling microorganisms in the food industry could be to use copper as an antimicrobial agent. The present study characterized three L. monocytogenes strains (List2-2, Apa13-2, and Al152-2A) adapted to low temperature and challenged with different copper concentrations. Similar MIC-Cu values were observed among studied strains, but growth kinetic parameters revealed that strain List2-2 was the least affected by the presence of copper at 8°C. This strain was selected for a global transcriptional response study after a 1 h exposition to 0.5 mM of CuSO 4 × 5H 2 O at 8 and 37°C. The results showed that L. monocytogenes apparently decreases its metabolism in response to copper, and this reduction is greater at 8°C than at 37°C. The most affected metabolic pathways were carbohydrates, lipids and nucleotides synthesis. Finally, 15 genes were selected to evaluate the conservation of the transcriptional response in the other two strains. Results indicated that only genes related to copper homeostasis showed a high degree of conservation between the strains studied, suggesting that a low number of genes is implicated in the response to copper stress in L. monocytogenes . These results contribute to the understanding of the molecular mechanisms used by bacteria to overcome a combination of stresses. This study concluded that the application of copper in low concentrations in cold environments may help to control foodborne pathogens as L. monocytogenes in the industry.

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“…Samples were processed as described by Latorre et al (2015). Samples were measured with a S2 PICOFOX TM TXRF spectrometer (Bruker, Germany) (González et al, 1999; Fiedor et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

The Combined Effect of Cold and Copper Stresses on the Proliferation and Transcriptional Response of Listeria monocytogenes

Quesille-Villalobos¹,

Parra²,

Maza³

et al. 2019

Front. Microbiol.

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“…Additionally, this work shows clearly the versatility, potential and sensitivity of TXRF spectrometry for the analytical evaluation of metals in this kind of microbiological systems. Fiedor et al [85] applied TXRF spectrometry to perform a comprehensive analysis of the trace elements content of purple non-sulfur phototrophic bacteria, their chromatophores and selected photosynthetic structures in response to alterations in oxygen growth conditions. There is a lack of consistent information on microelements' content, distribution, and correlations between them.…”

Section: Microbiological Systemsmentioning

confidence: 99%

TXRF spectrometry in the bioanalytical sciences: A brief review

Fernández-Ruiz

2021

X-Ray Spectrometry

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Funding informationUniversidad Aut onoma de Madrid This work describes the basic principles of Total-reflection X-Ray Fluorescence (TXRF) spectrometry and some of its most outstanding applications for investigating metal traces, mainly in biological and biomedical samples. TXRF is an analytical technique that combines the versatility of elemental analysis by X-ray energy dispersion with a low detection limit in the order of picograms of absolute mass or a few ppb (μg L À1 ) as concentration. The microanalytical capacity of TXRF allows the study of small sample quantities. The TXRF can perform qualitative and mass ratio analyses of the elements present in a sample of only a few hundred nanograms. These properties are of great importance in the research of metal traces in biological systems, as they allow fast, precise, and accurate characterisation of the elemental fingerprint at trace level. Its successful application in studies of the coordination kinetics of new platinumbased antitumor drugs with DNA, its application in the study profile of the metal in healthy and cancerous human tissues, or even its application in the study of the processes of metal diffusion through cell membranes are just a few examples of TXRF capabilities in the biomedical sciences. This brief review's main objective is to provide an overview of the physical principles and possibilities of TXRF spectrometry. It also highlights some of the most outstanding applications that have been developed so far, mainly in the biological field, so that the reader can evaluate their potential applications.

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“…Biological and clinical materials generally need to be pretreated to remove the organic matrix which would increase the background signal and thus worsen the detection limits. Common pretreatment methods are ashing, acid digestion, or purification using chromatographic methods [42][43][44][45][46][47][48][49][50]. Mineral oil, vegetable oil or fatty acid containing samples like creams and lotions can be diluted with a solvent or if necessary subjected to cold oxygen plasma ashing to remove excess carbon.…”

Section: Biological and Food Applicationsmentioning

confidence: 99%

Total reflection X-ray fluorescence

Schmeling

2019

Physical Sciences Reviews

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Abstract Total reflection X-ray fluorescence (TXRF) spectrometry is a non-destructive and surface sensitive multi-element analytical method based on energy dispersive X-ray fluorescence spectrometry with detection limits in the lower picogram range. It utilizes the total reflection of the primary X-ray beam at or below the critical angle of incidence. At this angle, the fluorescence intensity is substantially enhanced for samples present as small granular residue or as thin homogenous layer deposited at the surface of a thick substrate. Generally, two types of application exist: micro- and trace-analysis as well as surface and thin-layer analysis. For micro- and trace-analysis, a small amount of the solid or liquid sample is deposited on an optically flat substrate, typically quartz or polycarbonate. The dried residue is analyzed at a fixed angle setting slightly below the critical angle. Quantification is carried out by means of internal standardization. For surface and thin-layer analysis, the surface of an optically flat substrate is scanned. Variations of the incident angle of the primary X-ray beam provide information about the type and sometimes also the amount of material present at or slightly below the surface of the substrate. Major fields of application are environmental samples, biological tissues, objects of cultural heritage, semiconductors and thin-layered materials and films.

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Quantification of purple non-sulphur phototrophic bacteria and their photosynthetic structures by means of total reflection X-ray fluorescence spectrometry (TXRF)

Abstract: TXRF spectrometry proves useful in analysing bacterial cells and their substructures as exemplified byRhodobacter sphaeroides. Trace element profiling complemented with correlation analysis under varying growth conditions.

Cited by 7 publications

References 50 publications

The Combined Effect of Cold and Copper Stresses on the Proliferation and Transcriptional Response of Listeria monocytogenes

The Combined Effect of Cold and Copper Stresses on the Proliferation and Transcriptional Response of Listeria monocytogenes

TXRF spectrometry in the bioanalytical sciences: A brief review

Total reflection X-ray fluorescence

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