Influence of calcium hydroxide on dyes for dentin labeling, anlyzed by means of a new methodology

Moraes, Ivaldo Gomes de; Moraes, Fernanda Gomes de; Mori, Graziela Garrido; Gonçalves, Silvana Beltrami

doi:10.1590/s1678-77572005000300003

Cited by 7 publications

(6 citation statements)

References 9 publications

(4 reference statements)

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“…In relation to MTA, in the presence of water, calcium oxide in the material could form calcium hydroxide, which would certainly cause discoloration of methylene blue. In this study, a 0.2% Rhodamine B solution was used because according to Moraes, et al 23 (2005) and Tanomaru Filho, et al 27 (2005) , Rhodamine B is not influenced by alkaline materials.…”

Section: Discussionmentioning

confidence: 99%

“…The quality of apical sealing obtained by rootend filling materials has been assessed using different methodologies such as dye penetration 5,16,18,23,28,29,33 , bacterial penetration 3,14 , endotoxin 26 , human saliva penetration 2 and fluid filtration technique 17,21 . Studies on dye penetration were considered an easy method to evaluate root-end filling materials 4,33 .…”

Section: Introductionmentioning

confidence: 99%

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Sealing ability, marginal adaptation and their correlation using three root-end filling materials as apical plugs

et al. 2010

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ObjectiveThis study used dye leakage assay and scanning electron microscopy to evaluate, respectively, the sealing ability and marginal adaptation of three root-end filling materials used as apical plugs, as well as the possible correlation between these properties.Material and MethodsNinety-eight single-rooted human teeth were prepared to simulate an open apex. The teeth were allocated to three experimental groups (n = 30), which received a 5-mm thick apical plug of (1) gray MTA AngelusTM, (2) CPMTM and (3) MBPc, and two controls groups (n = 4). After immersion in 0.2% Rhodamine B solution for 48 h, the teeth were sectioned longitudinally and analyzed by Image Tool 3.0 software. The marginal adaptation between apical plugs and the root canal walls were analyzed by SEM.ResultsMBPc had significantly less (p<0.05) apical leakage than the other materials. Regarding marginal adaptation, CPMTM showed the best numerical results, though without statistical significance from the other materials (p<0.05). There was no correlation between the two properties.ConclusionsWhen used as apical plugs, the tested root-end filling materials had similar marginal adaptation to the dentin walls, but MBPc had the best sealing ability, as demonstrated by the least apical leakage from all tested materials.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sealing ability, marginal adaptation and their correlation using three root-end filling materials as apical plugs

et al. 2010

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“…Thus, discoloration of the surfaces stained by methylene blue may occur. Therefore, Rhodamine B dye solution is more appropriate for evaluating the sealing ability of MTA 22 , 24 .…”

Section: Discussionmentioning

confidence: 99%

In vitro sealing ability of white and gray mineral trioxide aggregate (MTA) and white Portland cement used as apical plugs

et al. 2007

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This study evaluated the sealing ability of apical plugs made of white and gray MTA-Angelus® and white Portland cement placed via the root canal and having different thicknesses (2, 5 and 7 mm). Ninety extracted human single-rooted teeth were instrumented using a size 40 K-file to standardize the foraminal opening by the stepback technique. The teeth were assigned to 3 groups (n=30), according to the material used for fabrication of the apical plugs: A = gray MTA; B = white MTA; C = white Portland cement. The groups were subdivided into groups of 10 teeth each according to the apical plug thickness (2, 5 and 7 mm). Marginal apical dye leakage was assessed using 0.2% Rhodamine B solution in which the specimens were immersed for 72 hours at 37°C. The roots were sectioned longitudinally in a buccolingual direction for apical plug exposure, and digital photographs were taken and analyzed by Image Tool image-analysis software. Data were analyzed statistically by Kruskal-Wallis and Dunn's tests. Significance level was set at 5%. The least percent leakage was observed for 5- and 7-mm-thick plugs (p<0.05). No significant difference (p>0.05) was found between gray MTA and white Portland cement. Among the three materials analyzed, white MTA presented the highest marginal leakage (p<0.05). The findings of the present study showed that gray MTA and Portland cement had better sealing ability than white MTA when used as apical plugs. Dye leakage was smaller for 5- and 7-mm-thick plugs compared to 2-mm-thick plugs.

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“…Moraes, et al 19 (2005) and Tanomaru Filho, et al 21 (2005) share the same opinion. Therefore, when performing the leakage test to assess the sealing ability of gray MTA- Angelus™, CPM™ and MBPc, in this study, 0.2% Rhodamine B was selected instead of methylene blue, based on the previously mentioned studies, since labeling by Rhodamine B is not influenced by alkaline materials 19 , 21 .…”

Section: Discussionmentioning

confidence: 83%

Sealing ability of grar MTA AngelusTM, CPM TM and MBPc used as apical plugs

et al. 2008

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This study evaluated the sealing ability of apical plugs fabricated with gray MTA Angelus™ sealer, CPM™ sealer and MBPc sealer. The root canals of 98 extracted single-rooted human teeth were instrumented with #5 to #1 Gates Glidden drills according to the crown-down technique until the #1 drill could pass through the apical foramen. The specimens were then prepared with K-files, starting with an ISO 50 until an ISO 90 could be visualized 1 mm beyond the apex. After root canal preparation, the external surface of each root was rendered impermeable and roots were assigned to 3 experimental groups (n = 30), which received a 5-mm thick apical plug of gray MTA Angelus™, CPM ™ and MBPc, and two control groups (n=4). The remaining portion of the canal in the experimental groups was filled by the lateral condensation technique. The teeth of each group, properly identified, were fixed on utility wax by their crowns and were placed in plastic flasks, leaving the apex free and facing upward. The flasks were filled with 0.2% Rhodamine B solution, pH 7.0, so as to completely cover the root apex of all teeth. The sealing ability was analyzed by measuring 0.2% Rhodamine B leakage after all groups had been maintained in this solution for 48 hours. Data were analyzed statistically by Kruskal-Wallis test and Dunn test with a=5%. The results showed that, among the tested materials used for fabrication of apical plugs, MBPc sealer had the least amount of leakage with statistically significant difference (p<0.05).

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Influence of calcium hydroxide on dyes for dentin labeling, anlyzed by means of a new methodology

Cited by 7 publications

References 9 publications

Sealing ability, marginal adaptation and their correlation using three root-end filling materials as apical plugs

Sealing ability, marginal adaptation and their correlation using three root-end filling materials as apical plugs

In vitro sealing ability of white and gray mineral trioxide aggregate (MTA) and white Portland cement used as apical plugs

Sealing ability of grar MTA AngelusTM, CPM TM and MBPc used as apical plugs

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