Influence of baking time and matrix effects on the detection of milk allergens in cookie model food system by ELISA

Monaci, Linda; Brohée, Marcel; Tregoat, Virginie; Hengel, Arjon van

doi:10.1016/j.foodchem.2010.12.113

Cited by 62 publications

(26 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The same amount of total DNA was used as the input in all reactions, and our results were 100% consistent with the absence or presence of milk, as indicated on the food label. These results demonstrate that the ability to detect bovine DNA using our Taq-Man MGB real-time PCR method was not affected by either the other ingredients, such as sugar and fat, in the foods or the processing procedures, such as baking (Monaci et al, 2011). Therefore, this method can be widely used for the detection of milk allergens in different types of food.…”

Section: Discussionmentioning

confidence: 75%

Development of a real-time quantitative PCR assay using a TaqMan minor groove binder probe for the detection of α-lactalbumin in food

Guan

Cai

Zhang

et al. 2016

Journal of Dairy Science

View full text Add to dashboard Cite

Here, we report the development of a real-time PCR assay using a TaqMan minor groove binder (MGB, Genecore, NCBI: AF249896.1, 806-820) probe and primer sets designed to recognize the α-lactalbumin gene from the cow (Bos taurus). We evaluated the efficacy of this assay for detecting and quantifying cow α-lactalbumin in commercial foods. Our results demonstrated that the developed method was highly sensitive and showed high specificity for cow milk, with consistent detection of 0.05 ng of bovine DNA. We tested 42 commercial food samples with or without cow milk listed as an ingredient by using the developed assay. Among the 42 samples, 26 products that listed milk as an ingredient and 3 products might contain milk showed positive signals, whereas the other 9 products that did not contain milk and 4 products that might contain milk tested negative. Therefore, this method could be widely used for the detection of cow milk allergens in food.

show abstract

Section: Discussionmentioning

confidence: 75%

Development of a real-time quantitative PCR assay using a TaqMan minor groove binder probe for the detection of α-lactalbumin in food

Guan

Cai

Zhang

et al. 2016

Journal of Dairy Science

View full text Add to dashboard Cite

show abstract

“…Food processing may deeply affect the protein structure depending on the intrinsic characteristics of the protein, the severity of the treatment applied, and the environment where it takes place. In particular, thermal processing has the potential to modify the structure, solubility, and immunochemical reactivity of allergen proteins, affecting the conformation, epitope antigenicity, and hydrophobicity [21,30,31].…”

Section: Varietal Diversity Andmentioning

confidence: 99%

Effects of the Varietal Diversity and the Thermal Treatment on the Protein Profile of Peanuts and Hazelnuts

Angelis

Bavaro

Monaci

et al. 2018

Journal of Food Quality

Self Cite

View full text Add to dashboard Cite

Several bu er compositions were compared for their e ciency in protein extraction from both raw and roasted peanut and hazelnut samples, the nal goal being to understand the modi cation of protein solubility upon roasting and maximize the extraction yield. Denaturant conditions provided by urea-TBS bu er resulted in satisfactory extraction yields for both peanut and hazelnut samples, before and after the thermal treatment. In addition, di erent varieties of peanuts and hazelnuts were characterized to highlight the extent of variability in the protein pro le accounted by the varietal factor and eventual di erential resistance among cultivars to protein modi cation induced by the thermal processing. e protein pro le was characterized by gel electrophoresis, and speci c bands were analyzed by micro-HPLC-MS/MS coupled to software-based protein identi cation. No signi cant di erence was observed for the investigated hazelnut cultivars, namely, Campana, Romana, and Georgia, whereas interesting features were presented for the peanut varieties Virginia, Zambia, and China. In particular, Zambia variety lacked two bands of approximately 36 and 24 kDa that were visible in Virginia and China varieties, which could suggest a lower allergenic potential of this particular variety which deserves to be further investigated before drawing nal conclusions.

show abstract

“…If they fail to coincide, and instead the calculated values diverge by amounts corresponding to the difference between the dilution factors, this would constitute a strong indicator of a contaminating presence binding specifically to the antibody. Matrix effects, especially at low dilutions, are assumed to be occurring when immunological methods are used [41–43]. These effects should be reduced with dilution, as the typical matrix effect is presumed to result from concentrated nonspecific plasma proteins.…”

Section: Discussionmentioning

confidence: 99%

“…These effects should be reduced with dilution, as the typical matrix effect is presumed to result from concentrated nonspecific plasma proteins. Dilution of samples is a common remedy for suspected matrix effects [43]. Unfortunately, the individual results of several dilutions of each sample are rarely reported in endocrine disruption studies.…”

Section: Discussionmentioning

confidence: 99%

Affinity and Matrix Effects in Measuring Fish Plasma Vitellogenin Using Immunosorbent Assays: Considerations for Aquatic Toxicologists

Bartell

Schoenfuss

2012

ISRN Toxicology

View full text Add to dashboard Cite

Enzyme-linked immunosorbent assays (ELISAs) are important tools in aquatic toxicology and have become crucial in assessing exposure concentrations in the aquatic environment and acute physiological responses in exposed organisms. These assays utilize the inherent properties of antibodies to recognize and selectively bind a target molecule, while largely ignoring other molecules to provide semiquantitative values. A variety of methodologies to measure plasma vitellogenin using ELISAs have generated widely divergent data. Limitations of the ELISA method are known in the wider immunology field, though aquatic toxicologists may be less familiar with these limitations. We evaluated several mechanisms contributing to the divergent vitellogenin data in the literature. Antibody affinities and the matrix in which standard curves are constructed are possible error generators. These errors can be amplified by large sample dilutions necessary to fall within the standard curve. It is important for the aquatic toxicology research community to realize the limitations and understand the pitfalls of absolute plasma vitellogenin data in their studies.

show abstract

Influence of baking time and matrix effects on the detection of milk allergens in cookie model food system by ELISA

Cited by 62 publications

References 31 publications

Development of a real-time quantitative PCR assay using a TaqMan minor groove binder probe for the detection of α-lactalbumin in food

Development of a real-time quantitative PCR assay using a TaqMan minor groove binder probe for the detection of α-lactalbumin in food

Effects of the Varietal Diversity and the Thermal Treatment on the Protein Profile of Peanuts and Hazelnuts

Affinity and Matrix Effects in Measuring Fish Plasma Vitellogenin Using Immunosorbent Assays: Considerations for Aquatic Toxicologists

Contact Info

Product

Resources

About