Influence of Bacillus Megaterium on Crack Healing and Mechanical Properties of Concrete

Attri, Rishab; Shukla, Ayush; Sharma, Vinayak; Thakur, Ayush

doi:10.1007/978-3-030-51485-3_10

Cited by 3 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As can be seen from Figure 6, comparing the culture trials conducted by Sun et al [29] using LB nutrient solution and the improved nutrient solution adopted by Attri et al [37] for Bacillus megaterium, the No. 7 nutrient solution used in this thesis reached an absorbance OD 600 of 1.2 after 24 h and 1.7 after 48 h, which was 2.1 times higher than LB nutrient solution and 1.7 times higher than ATTRI nutrient solution, respectively.…”

Section: Determining the Optimal Culturementioning

confidence: 95%

“…Bacillus megaterium is a member of the Bacillus family that efciently converts urea into ammonium carbonate, which in turn forms calcium carbonate precipitates [37]. In this paper, Bacillus megaterium is used as an example of a method to enhance the microorganism's ability to perform MICP in a strong alkaline environment through domestication studies.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Study of Concrete Crack Repair using Bacillus megaterium

Shi

Wang

et al. 2022

Advances in Materials Science and Engineering

View full text Add to dashboard Cite

The low yield of microbial calcium carbonate precipitation due to the strong pH environment has limited the development of biotechnology-based concrete crack repair techniques. In this study, Bacillus megaterium was selected to determine the optimal nutrient formulation through orthogonal tests. The growth and reproduction characteristics and urease activity of this strain were analysed by controlling different pH values, and the calcium carbonate precipitation yield under different pH conditions was investigated, and the lower precipitation yield under strong alkaline conditions was improved by using alkaline environment domestication with the addition of urea to the nutrient solution. It has been shown that higher pH results in lower growth and multiplication of Bacillus megaterium and weaker urease activity, and a strong alkaline environment can significantly suppress its growth and multiplication and urease activity. At pH 7, the growth and multiplication of Bacillus megaterium were the fastest, the urease activity was the strongest, and the precipitation yield was greater. The addition of urea to the nutrient solution to domesticate Bacillus megaterium in an alkaline environment can significantly increase the growth rate and precipitation yield, which can effectively solve the problem of insufficient calcium carbonate precipitation under alkaline conditions. Comparing the effectiveness of microbially induced calcium carbonate precipitation (MICP) in repairing concrete after damage before and after microbial domestication shows that the domesticated Bacillus megaterium is more effective in reducing the permeability characteristics of concrete and improving its strength.

show abstract

Section: Determining the Optimal Culturementioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Study of Concrete Crack Repair using Bacillus megaterium

Shi

Wang

et al. 2022

Advances in Materials Science and Engineering

View full text Add to dashboard Cite

show abstract

“…Self-healing concrete is an innovation that utilizes healing agents in the form of microorganisms, namely bacteria with high alkaline properties, to survive in extreme environments such as the genus Bacillus [9], [10]. When cracks occur, moisture and oxygen enter the fracture zone, and active bacteria and nutrient precursors, namely calcium lactate, are dissolved from the microbial particles to form a suitable environment for spores to germinate and begin to precipitate calcium carbonate (CaCO 3).…”

Section: Introductionmentioning

confidence: 99%

Enhancing Performance of High-Strength Concrete Using Bacillus Megaterium as Self-Healing Agent

Mauludin

2024

GEOMATE

View full text Add to dashboard Cite

The weak tensile properties of concrete allowed micro-cracks to emerge and propagate into macrocracks if they were left undetected. An innovation called self-healing concrete (SHC) was subsequently introduced, allowing concrete to cover micro-cracks independently without any human intervention. SHC utilizes healing agents in the form of bacteria, which react with oxygen and water when cracks occur, resulting in calcium carbonate (CaCO3) compounds that play a role in the concrete repair process. The aim of this study is to determine the effects of Bacillus megaterium on the compressive strength, flexural strength, depth of water penetration, and velocity of the 45 MPa high-strength concrete design based on ACI 211-4r.93 guidelines, with 25% of cement substitution by fly ash and 2% of bacteria percentage of the entire water composition. A 12.662% increase in compressive strength and a 1.690% increase in flexural strength were recorded for the age of 28 days bacterial concrete compared to the control concrete. The water penetration depth of the bacterial concrete was 4.49 cm, thereby meeting the DIN 1045 standard, which is less than 5 cm. From the ultrasonic pulse velocity (UPV) test, the average velocity value for bacterial concrete was 9.483 km/sec, which is categorized as having good concrete homogeneity based on the IS 1331 standard. This research offers promising insights into the SHC field by adding innovation to the design of high-quality bacterial concrete.

show abstract