Study of Concrete Crack Repair using Bacillus megaterium

Shi, Wenbo; Wang, Meng; Wu, Lan; Xie, Xinhang; Wang, Miao; Lu, Tianjing

doi:10.1155/2022/6188680

Cited by 3 publications

(1 citation statement)

References 48 publications

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“…Bacteria would adhere to the crack surface during the repair by MICP. Biofilm is an extracellular polymeric substance, which is produced by attached bacteria [40][41][42]. Previously conducted laboratory-based experiments do not considered biofilm, but this does not mean that no biofilm exists in experiments.…”

Section: Introductionmentioning

confidence: 99%

A Novel Mathematical Model for Repairing Rough Cracks Using the Microbially Induced Carbonate Precipitation (MICP)

Zhang,

Wang,

Ahmed

et al. 2023

Sustainability

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Concrete cracks have an adverse effect on the strength properties and durability of concrete structures. Therefore, repairing concrete cracks to recover the concrete’s strength parameters is an important task in the civil engineering field. For repairing concrete cracks, the MICP technique has been widely analyzed in recent times; however, no research has been conducted to deeply investigate the repair effects of MICP on concrete cracks with a rough surface using a theoretical model. In the current research, MICP with a novel mathematical model was conducted considering the precipitation of calcium carbonate (CaCO3), ureolysis, suspended biomass, geochemistry, transport of solutes, and biofilm growth. Furthermore, crack repair experiments were performed to assess the performance of the new mathematical model. The results revealed that the calculated concentrations of suspended biomass in cracks gradually decreased during the test. The comparison between the experimental results and calculated results verified the precision of the migration behavior of the suspended biomass. At the inlet, the solute concentrations and volume fractions of biofilm were higher, causing an increase in the productive rates of calcium carbonate. The consumed concentrations of solutes were higher for cracks with a smoother surface, eventually leading to smaller values of sonic time; the upper parts of the cracks also had smaller values of sonic time, showing good repair effects. The proposed mathematical model provides a better solution to control the repair time and microbial metabolism process, allowing for adjustive bioremediation and biomineralization of concrete, which could provide a firm basis for the remediation of materials in the civil engineering field.

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Section: Introductionmentioning

confidence: 99%

A Novel Mathematical Model for Repairing Rough Cracks Using the Microbially Induced Carbonate Precipitation (MICP)

Zhang,

Wang,

Ahmed

et al. 2023

Sustainability

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Microbially induced calcium carbonate precipitation through CO2 sequestration via an engineered Bacillus subtilis

Gilmour,

Ghimire,

Wright

et al. 2024

Microb Cell Fact

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Background Microbially induced calcium carbonate precipitation has been extensively researched for geoengineering applications as well as diverse uses within the built environment. Bacteria play a crucial role in producing calcium carbonate minerals, via enzymes including carbonic anhydrase—an enzyme with the capability to hydrolyse CO2, commonly employed in carbon capture systems. This study describes previously uncharacterised carbonic anhydrase enzyme sequences capable of sequestering CO2 and subsequentially generating CaCO3 biominerals and suggests a route to produce carbon negative cementitious materials for the construction industry. Results Here, Bacillus subtilis was engineered to recombinantly express previously uncharacterised carbonic anhydrase enzymes from Bacillus megaterium and used as a whole cell catalyst allowing this novel bacterium to sequester CO2 and convert it to calcium carbonate. A significant decrease in CO2 was observed from 3800 PPM to 820 PPM upon induction of carbonic anhydrase and minerals recovered from these experiments were identified as calcite and vaterite using X-ray diffraction. Further experiments mixed the use of this enzyme (as a cell free extract) with Sporosarcina pasteurii to increase mineral production whilst maintaining a comparable level of CO2 sequestration. Conclusion Recombinantly produced carbonic anhydrase successfully sequestered CO2 and converted it into calcium carbonate minerals using an engineered microbial system. Through this approach, a process to manufacture cementitious materials with carbon sequestration ability could be developed.

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A Novel Mathematical Model for Rough Cracks Repairing by Using the Method of Microbially Induced Carbonate Precipitation (MICP)

Zhang,

Wang,

Ahmed

et al. 2023

Preprint

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Concrete cracks have a detrimental effect on the strength properties and durability of the structures. So, repairing of concrete cracks to recover their strength parameters is more important task in civil engineering. To repair concrete cracks, MICP has been widely analysed in recent times; but, zero research is conducted to deeply investigate the repair effects of MICP on concrete cracks with rough surface by using the theoretic model. In current research, MICP with a novel mathematical model was attained by taking the precipitation of calcium carbonate (CaCO3), ureolysis, suspended biomass, geochemistry, transport of solute and biofilm growth. Furthermore, crack repair experiments were performed to check the workability of the new mathematical method. The outcomes revealed that the designed suspended biomass concentrations in cracks diminished step by step. The comparison in between the experimental results and calculated results verified the precision of migration behaviour of the suspended biomass. At the inlet, the solute concentrations and biofilm volume fractions was higher, causing in rise of yield amounts for calcium carbonate. The consumed solutes concentrations were higher for cracks of less rough surface, ultimately causing in smaller sonic time; and the values of sonic time for upper portions of cracks was smaller, showing good repair impacts. The recommended mathematical model provides a better tool that controls repair time and microbial metabolism process to report a new adjustive, bioremediation and biomineralization to concrete, which could provide a firm base for remediation of material in civil engineering field.

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Study of Concrete Crack Repair using Bacillus megaterium

Cited by 3 publications

References 48 publications

A Novel Mathematical Model for Repairing Rough Cracks Using the Microbially Induced Carbonate Precipitation (MICP)

A Novel Mathematical Model for Repairing Rough Cracks Using the Microbially Induced Carbonate Precipitation (MICP)

Microbially induced calcium carbonate precipitation through CO2 sequestration via an engineered Bacillus subtilis

A Novel Mathematical Model for Rough Cracks Repairing by Using the Method of Microbially Induced Carbonate Precipitation (MICP)

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