Influence of aqueous aluminium on the immune system of the freshwater crayfish Pacifasticus leniusculus

Ward, Richard J. S.; McCrohan, Catherine R.; White, Kenneth

doi:10.1016/j.aquatox.2005.12.006

Cited by 68 publications

(42 citation statements)

References 25 publications

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“…Environmental stressors may suppress the immune responses in signal crayfish (Ward et al 2006), although the stressors in the case of Lake Immeln, where the caging experiment took place and ESS was high among female signal crayfish, are unknown. We hypothesize that reproductive impairment caused by ESS may be among the factors explaining the population collapses in Lake Immeln, and potentially in other lakes as well.…”

Section: Discussionmentioning

confidence: 99%

Eroded swimmeret syndrome in female crayfish Pacifastacus leniusculus associated with Aphanomyces astaci and Fusarium spp. infections

Edsman¹,

Nyström²,

Sandström³

et al. 2015

Dis. Aquat. Org.

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Section: Discussionmentioning

confidence: 99%

Eroded swimmeret syndrome in female crayfish Pacifastacus leniusculus associated with Aphanomyces astaci and Fusarium spp. infections

Edsman¹,

Nyström²,

Sandström³

et al. 2015

Dis. Aquat. Org.

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“…Samples were used for determining the total haemocyte counts (THCs) and for haemolymph biochemical analyses. Total haemocyte counts were performed by modifications of the methods of Miller and Stanley (21), Evans (22), and Ward et al (23). After haemolymph samples were centrifuged at 2500 g, 10 min at 4 °C , biochemical analyses were done by standard analytical techniques.…”

Section: Haemolymph Analysismentioning

confidence: 99%

The sublethal effects of (2,4-Dichlorophenoxy) acetic acid (2,4-D) on narrow-clawed crayfish (Astacus leptodactylus Eschscholtz, 1823)

Benlı

Şahin

Sarıkaya

et al. 2016

Archives of Industrial Hygiene and Toxicology

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2,4-D is a widely used phenoxy herbicide, potentially toxic to humans and biota. The objective of the present study was to reveal short term sublethal effects of 2,4-D on narrow-clawed freshwater crayfish (Astacus leptodactylus Eschscholtz, 1823), based on histology, total haemocyte counts, selected haemolymph parameters, and oxidative stress parameters. In the laboratory conditions crayfish specimens were exposed to 9 mg L -1 of 2,4-D for one week. Experiments were conducted under semi-static conditions in 20 L-capacity aquaria where 10 freshwater crayfish were stocked per aquarium. Exposure (experimental) and control groups were used and the experiments were repeated two times. No mortality and behavioural changes were recorded during the experiments. Total haemocyte counts decreased significantly, while haemolymph glucose levels increased (P<0.05), when compared to the control group. Haemolymph levels of calcium, chloride, sodium, potassium, magnesium, total protein, and lactate did not change. Exposure resulted with increased levels of malondialdehyde (MDA) only in hepatopancreas. However, results of gill FOX assay showed a significant decrease in oxidative stress parameters (P<0.05). MDA levels of gill and abdominal muscle tissues and FOX levels of hepatopancreas and abdominal muscle tissues did not change when compared to the control group. Significant histopathological alterations were observed both in hepatopancreas (multifocal deformations in tubule lumen) and gill tissue (melanisation of gill lamella). Exposure of crayfish even to a sublethal concentration of 2,4-D alters histopathology and lipid peroxidation due to stress. Biomarkers studied here seem to be useful for the assessment of adverse/toxic effects of pesticides on non-target, indicator aquatic organisms.KEY WORDS: haemolymph; herbicide; histology; oxidative stress; toxicity T h e f i r s t s u c c e s s f u l s e l e c t i v e h e r b i c i d e , 2,4-dichlorophenoxy acetic acid (2,4-D), was developed in 1946. It belongs to the phenoxy herbicide family and is still one of the systemic herbicides widely used to control many types of broadleaf weeds (1). It is used to control aquatic vegetation, in pasture and rangeland applications, in cultivated agriculture, forest management, home, and garden. It acts by sustaining high levels of the plant hormone auxin, which results in overstimulation of plant growth and death. In addition to this, it causes changes in the animal nervous system based on receptor interaction/interference of acetylcholine. Furthermore, it inhibits the acetyl cholinesterase (AChE) activity and increases the level of serotonin.Available data on 2,4-D toxicity on aquatic organisms mostly rely on the reports of its acute toxic effects observed in different fish species. Oruc and Uner (4) studied the combined effects of azinphosmethyl and 2,4-D on Oreochromis niloticus to clarify the mode of its action on the cellular defence system. Farah et al. (5) (8); for catfish (Clarias batrachus) by studying micronuclei and erythrocyte alt...

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“…Clearwater et al (2014) demonstrate that fingernail clam survival is adversely affected by high dosage (344 g alum/m 2 ) of alum application and some aluminum accumulation occurred in the crayfish and mussels (Clearwater et al, 2014). The aqueous Al can increase the risk of infection in the crayfish by impairing the ability of haemocytes to recognise and/or remove bacteria from the circulation (Ward et al, 2006). Recent studies indicate that toxic Al 3þ could be released after alum application at low pH (<6.0), and sediment-capping with alum could inhibit microbial nitrification and denitrification under aerobic conditions (Gibbs and Oezkundakci, 2011).…”

Section: Introductionmentioning

confidence: 99%

Ecotoxicological assessment of flocculant modified soil for lake restoration using an integrated biotic toxicity index

2016

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a b s t r a c tFlocculant modified soils/clays are being increasingly studied as geo-engineering materials for lake restoration and harmful algal bloom control. However, the potential impacts of adding these materials in aquatic ecological systems remain unclear. This study investigated the potential effects of chitosan, cationic starch, chitosan modified soils (MS-C) and cationic starch modified soils (MS-S) on the aquatic organisms by using a bioassay battery. The toxicity potential of these four flocculants was quantitatively assessed using an integrated biotic toxicity index (BTI). The test system includes four aquatic species, namely Chlorella vulgaris, Daphnia magna, Cyprinus carpio and Limnodrilus hoffmeisteri, which represent four trophic levels in the freshwater ecosystem. Results showed that median effect concentrations (EC 50 ) of the MS-C and MS-S were 31e124 times higher than chitosan and cationic starch, respectively. D. magna was the most sensitive species to the four flocculants. Histological examination of C. carpio showed that significant pathological changes were found in gills. Different from chitosan and cationic starch, MS-C and MS-S significantly alleviated the acute toxicities of chitosan and cationic starch. The toxicity order of the four flocculants based on BTI were cationic starch > chitosan > MS-S > MS-C. The results suggested that BTI can be used as a quantitative and comparable indicator to assess biotic toxicity for aquatic geo-engineering materials. Chitosan or cationic starch modified soil/clay materials can be used at their optimal dosage without causing substantial adverse effects to the bioassay battery in aquatic ecosystem.

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Influence of aqueous aluminium on the immune system of the freshwater crayfish Pacifasticus leniusculus

Cited by 68 publications

References 25 publications

Eroded swimmeret syndrome in female crayfish Pacifastacus leniusculus associated with Aphanomyces astaci and Fusarium spp. infections

Eroded swimmeret syndrome in female crayfish Pacifastacus leniusculus associated with Aphanomyces astaci and Fusarium spp. infections

The sublethal effects of (2,4-Dichlorophenoxy) acetic acid (2,4-D) on narrow-clawed crayfish (Astacus leptodactylus Eschscholtz, 1823)

Ecotoxicological assessment of flocculant modified soil for lake restoration using an integrated biotic toxicity index

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