Influence of anticoagulants and blood storage on hematological values in tambaqui, Colossoma macropomum

Tavares‐Dias, Marcos; Sandrim, Valéria C.

doi:10.4025/actascibiolsci.v20i0.4465

Cited by 14 publications

(9 citation statements)

References 25 publications

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“…Porém, foi maior do que o encontrado por Silveira & Rigores (1989) ao trabalhar em O. aureus, e por Wepener et al (1992) ao avaliar Tilapia sparrmanii. Todavia, tais discrepâncias entre tilápias do mesmo gênero provavelmente se devem a escolha do anticoagulante utilizado na colheita das amostras, em que amostras oriundas de plasma heparinizado são maiores quando comparados às amostras obtidas com a utilização de EDTA (Tavares-Dias & Sandrim 1998).…”

Section: Discussionunclassified

Parâmetros hematológicos e bioquímicos da tilápia-do-Nilo (Oreochromis niloticus L.) sob estresse por exposição ao ar

et al. 2012

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Pesq. Vet. Bras. 32(Supl. The present study evaluated the hematological and biochemical parameters of adult tilapia (Oreochromis niloticus) under the influence of the physiological stress factor in animals submitted to air exposure during fattening in raceway system. Blood cell count, hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), leukogram, differencial leukocyte count, platelet, glucose, total protein, cholesterol, triglycerides and electrolytes (calcium, chloride, sodium and potassium) were analyzed. The results showed a uniform distribution for red blood cells, hematocrit, hemoglobin, secondary Wintrobe indices, total protein, glucose, cholesterol, and serum ions, indicated by a relatively low variation coefficient. There was positive correlation only for total white blood cells, organic defense cells (neutrophils and lymphocytes), glucose, cholesterol, sodium and calcium. As for the leukocytes (WBC), as the animals were exposed to aeration, the number of leukocytes gradually decreased (leukopenia), simultaneously occurring neutrophilia and lymphopenia. The glycemic index was a good indicator of physiological stress due to hyperglycemia (82.0+20.88mg/dL) demonstrated in the treatments. The air exposure constituted an imbalance factor at the ion homeostasis and at the endogenous cholesterol synthesis. Besides, the recovery time did not result in the complete physiological rehabilitation face the imposed challenge.

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Section: Discussionunclassified

Parâmetros hematológicos e bioquímicos da tilápia-do-Nilo (Oreochromis niloticus L.) sob estresse por exposição ao ar

et al. 2012

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“…No entanto, alguns anticoagulantes utilizados podem determinar limitações durante o processamento das amostras sanguíneas, ocasionando alterações in vitro na determinação dos parâmetros hematológicos (HATTINGH, 1975;VAN VLIET et al, 1985;TAVARES-DIAS & SANDRIM, 1998; WALENCIK & WITESKA, Ciência Rural, v.40, n.7, jul, 2010. (1985) citam que a utilização de anticoagulante eficiente e adequado torna-se essencial para os procedimentos hematológicos e imunológicos.…”

Section: Introductionunclassified

Heparina e Na2EDTA como anticoagulantes para surubim híbrido (Pseudoplatystoma reticulatum x P. corruscans): eficácia e alterações hematológicas

et al. 2010

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“…Number of red blood cells and hemoglobin concentration, otherwise, differ from Das and Mukherjee (2003) and Adhikari et al (2004), who demonstrated reduction in these parameters for Labeo rohita exposed to stressor agents. It can be assumed that, for dourado, semi-natural and extruded methodologies do not alter eritropoietic process, besides that, for the same species, different results for hemoglobin concentration and hematocrit can be caused by blood samples collection techniques, including anticoagulants and anesthetic use, time elapsed between collection and laboratorial analysis, among others (Tavares-Dias and Sandrim, 1998). More studies are needed to the understanding of these differences.…”

Section: Resultsmentioning

confidence: 99%

Stress evaluation in dourado females (Salminus brasiliensis) submitted to two different methods of induced spawning

Zanoni¹,

Carvalho²,

Costa³

et al. 2019

Int. J. Fish. Aquac.

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The goals of this study were to evaluate dourado female egg viability submitted to stress condition caused by two different reproductive induction methods, extruded and semi-natural. Sixteen females randomly chosen were induced with pituitary extract and allocated in two groups; the first, in which oocytes were manually extruded and the second group, females were allowed to naturally spaw. Blood samples were collected for glucose, cortisol and hematocrit determinations as well as for red blood cells and white blood cells smears evaluations. A control group had its blood collected before hormonal induction. Fertilization rate was 40.6 and 91.7% for extruded and semi-natural methods, respectively, and the survival rate was higher in semi-natural groups. Both experimental groups showed higher glucose and cortisol levels compared to control group. The results for hematocrit, hemoglobin concentration and erythrocytes numbers did not differ among groups. Extruded and seminatural procedures elevated monocytes percentage. In conclusion, the semi-natural procedures are more efficient than extruded method and should be taken into account for Salminus brasiliensis handling of breeders.

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Influence of anticoagulants and blood storage on hematological values in tambaqui, Colossoma macropomum

Cited by 14 publications

References 25 publications

Parâmetros hematológicos e bioquímicos da tilápia-do-Nilo (Oreochromis niloticus L.) sob estresse por exposição ao ar

Parâmetros hematológicos e bioquímicos da tilápia-do-Nilo (Oreochromis niloticus L.) sob estresse por exposição ao ar

Heparina e Na2EDTA como anticoagulantes para surubim híbrido (Pseudoplatystoma reticulatum x P. corruscans): eficácia e alterações hematológicas

Stress evaluation in dourado females (Salminus brasiliensis) submitted to two different methods of induced spawning

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