Influence of antibody valency in a displacement immunoassay for the quantitation of 2,4-dichlorophenoxyacetic acid

Gerdes, Melanie; Meusel, Markus; Spener, Friedrich

doi:10.1016/s0022-1759(98)00211-7

Cited by 16 publications

(16 citation statements)

References 30 publications

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“…PCR or RT-PCR is only an indirect and semiquantitative measure of an infection by defective virus. Due to the defective genome, only cells productively coinfected with a helper virus can be quantified by a recently established immunofocus assay using a specific antiserum to the gag p12 portion of the defective virus [17]. The fact that blood can transfer MAIDS [13]and that the ecotropic and MCF virus-infected and infectious cells can be detected in quantities and infection frequencies similar to lymphoid organ suggests that infectious pseudotyped defective virus can also be released by blood cells.…”

Section: Discussionmentioning

confidence: 99%

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Murine AIDS Induces Viremia and Functional and Phenotypic Alterations in Blood Cells

Hügin¹,

Wirth²

2002

Int Arch Allergy Immunol

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Background: Murine acquired immunodeficiency syndrome (MAIDS) is characterized by generalized lymphoproliferation and progressive immunodeficiency. It is induced by a mixture of two replication-competent murine leukemia viruses (MuLV) and a disease-causing, replication-incompetent defective MuLV. Infection leads to specific phenotypic and functional alterations of lymphocytes in lymphoid organs. Methods: We analyzed phenotypic, virological and functional parameters in the blood of mice infected with MAIDS virus. Results: Disease progression correlated with increasing viremia, a loss of mitogen responsiveness of T lymphocytes, and the appearance of CD4+ Thy1– T lymphocytes. At >9 weeks after infection, the distribution of leukocyte cell populations became very heterogeneous, and late-stage leukemic events were observed in 5 of 23 mice. Conclusions: Virus titers, mitogen responsiveness and the presence of CD4+ Thy1– T lymphocytes can efficiently be monitored in the blood and serve as diagnostic parameters to monitor disease progression. Acute leukemic events occurring at the terminal stage could be responsible for the death of at least some of the mice with MAIDS.

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Section: Discussionmentioning

confidence: 99%

“…Titration of ecotropic and MCF virus or of productively infected cells was done with an immunochemical focus assay [17]. Blood from infected mice was obtained from the tail vein and was heparinized to prevent clotting.…”

Section: Methodsmentioning

confidence: 99%

Murine AIDS Induces Viremia and Functional and Phenotypic Alterations in Blood Cells

Hügin¹,

Wirth²

2002

Int Arch Allergy Immunol

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“…Improved signal intensity and reproducibility, background reduction and a more stable probe layer have been addressed as advantages with respect to the classic protein-hapten conjugate adsorption coating strategy (Ivanova et al, 2006;Ortega-Vinuesa et al, 1995). Moreover, different authors supported that direct hapten coated format showed an improvement in immunoassay sensitivity as well; this probably due to changes in the valency of antibody-hapten interaction (Gerdes et al, 1999;Townsend et al, 2006). In line with this, several approaches have been developed on modified polystyrene (PS) microtriter wells of ELISA plates, resulting in improved analytical performances of the assays (Böcher and Sorensen, 1994;Feng et al, 2009;Kaur et al, 2008;Holthues et al, 2005;Niveleau et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Direct hapten-linked multiplexed immunoassays on polycarbonate surface

Tamarit-López¹,

Morais²,

Puchades³

et al. 2011

Biosensors and Bioelectronics

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Direct hapten-linked multiplexed immunoassay is developed on the polycarbonate surface of standard Digital Versatile Discs (DVDs) for six compounds of environmental concern, as proof of concept. Carboxylated haptens are directly linked to the aminated polycarbonate surface through carbodiimide/succinimide coupling. The modified DVD surface maintained its physical and optical properties. Multiplexed assay reached detection limits down to 0.1 μg/L for chlorpyrifos, 2,4,5-trichlorophenoxypropionic acid, sulfathiazole and sulfasalazine and down to 1.0 μg/L for fenthion and malathion. This approach presents advantages such as the improvement in sensitivity in comparison to protein-hapten conjugate format for all the studied analytes and the absence of cross-interference effects, allowing high throughput multianalysis on the same surface. Also, a comparison of the performance of two sensing strategies indicated that DVD disc and drive approach turned out in a simpler mode, the assays being more reproducible and with higher signal to noise ratios.

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“…Apart from reductions in costs, recombinant fragments provide several other advantages for biosensing processes, including the removal of animal sacrifices, decreases in non-specific binding and the advantage of monovalency. Several publications describe the production of such fragments to environmental pollutants but only a few describe the production of fragments to low molecular weight haptens such as the pesticides atrazine [25,[27][28][29], paraquat [25,28] mecoprop [25], diuron [25,30] 2,4-D [31], chlorpyrifos [32] and chlorpyrifos-ethyl [33]. In this work, recombinant single-chain antibody (scAb) fragments to the pesticide atrazine, which have been shown to be capable of as sensitive a detection as their whole antibody counterparts, were employed [28].…”

Section: Introductionmentioning

confidence: 99%

Atrazine analysis using an amperometric immunosensor based on single-chain antibody fragments and regeneration-free multi-calibrant measurement

Grennan

Strachan²,

Porter³

et al. 2003

Analytica Chimica Acta

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This work describes the development of an electrochemical immunosensor for the analysis of atrazine using recombinant single-chain antibody (scAb) fragments. The sensors are based on carbon paste screen-printed electrodes incorporating the conducting polymer polyaniline (PANI)/poly(vinylsulphonic acid) (PVSA), which enables direct mediatorless coupling to take place between the redox centres of antigen-labelled horseradish peroxidase (HRP) and the electrode surface. Competitive immunoassays can be performed in real-time using this separation-free system. Analytical measurements based on the pseudo-linear relationship between the slope of a real-time amperometric signal and the concentration of analyte, yield a novel immunosensor setup capable of regenerationless amperometric analysis. Multiple, sequential measurements of standards and samples can be performed on a single scAb-modified surface in a matter of minutes. No separation of bound and unbound species was necessary prior to detection. The system is capable of measuring atrazine to a detection limit of 0.1 ppb (0.1 g l −1). This system offers the potential for rapid, cost-effective immunosensing for the analysis of samples of environmental, medical and pharmaceutical significance.

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Influence of antibody valency in a displacement immunoassay for the quantitation of 2,4-dichlorophenoxyacetic acid

Cited by 16 publications

References 30 publications

Murine AIDS Induces Viremia and Functional and Phenotypic Alterations in Blood Cells

Murine AIDS Induces Viremia and Functional and Phenotypic Alterations in Blood Cells

Direct hapten-linked multiplexed immunoassays on polycarbonate surface

Atrazine analysis using an amperometric immunosensor based on single-chain antibody fragments and regeneration-free multi-calibrant measurement

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