SUMMARY
The glucosinolates are thought to contribute to resistance to pests and diseases in members of the Cruciferae, including oilseed rape (Brassica napus) and they are known to accumulate in Brassica tissues after infestation by various pests. The present study investigated the changes in glucosinolate concentration in leaves of oilseed rape following inoculation with the dark leaf spot pathogen (Alternaria brassicae). Fourth and sixth leaves of the single‐low cultivar Bienvenu (low in erucic acid) and the double‐low cultivar Cobra (low in erucic acid and glucosinolate) were removed at intervals up to twenty days after inoculation and analysed for glucosinolate content using HPLC. Glucosinolates accumulated in inoculated leaves of both cultivars but the accumulation was greater for cv. Bienvenu, especially in sixth leaves. Among the glucosinolates, aliphatic compounds accumulated rapidly in cv. Bienvenu, but later declined. Indolyl and aromatic glucosinolates accumulated in both cultivars, but at a slower rate than the aliphatic glucosinolates. There were differences in the extent to which individual glucosinolates accumulated after inoculation. Disease symptoms were initially more extensive on cv. Cobra than on cv. Bienvenu but were similar on corresponding leaves of the two cultivars by the end of the experiment. However, sixth leaves had significantly less lesioning than fourth leaves. Glucosinolate accumulation in infected oilseed rape may restrict the spread of existing fungal infection or inhibit subsequent attempted infections, especially in younger leaves.
SUMMARY
The glucosinolate content of oilseed rape {Brassica napus) leaves was monitored over the growth period 30–70 days after planting, and a comparison made between a single‐low cultivar (low in erucic acid), Bienvenu, and a double‐low cultivar (low in erucic acid and glucosinolate), Cobra. In older, fully‐expanded leaves the glucosinolate concentration was very low (< 0.3 μmol/ml tissue water) and did not alter during the course of the experiment. In developing sixth leaves glucosinolate content increased rapidly and reached a maximum concentration (4–5 μmol/ml tissue water) 40 days after planting (6 days after leaf emergence). The concentration then declined, to about 1 μmol/ml after 60 days although the total glucosinolate content in leaves continued to increase until 50 days; much of the reduction in concentration was simply a result of leaf expansion. No major differences were seen between the two varieties in total glucosinolate content or in the individual compounds present. Cv. Cobra developed more quickly than cv. Bienvenu so direct comparison between leaves of the two cultivars was complex. When comparing the glucosinolate content of oilseed rape leaves, between cultivars or between treatments, it is vital to ensure that carefully matched leaves of comparable developmental age are selected.
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