Influence of antibody immobilization strategies on the analytical performance of a magneto-elastic immunosensor for Staphylococcus aureus detection

Menti, C.; Beltrami, M.; Pozza, Márcia Dalla; Martins, Sandro Tomaz; Henriques, João Antônio Pêgas; Santos, Alexandre Leme Godoy dos; Missell, F.P.; Roesch‐Ely, Mariana

doi:10.1016/j.msec.2017.03.107

Cited by 13 publications

(8 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Examples of bioreceptors are enzymes, antibodies, nucleic acids, and aptamers. Regardless of recognition elements chosen, the functionalization step, i.e., the immobilization of bioreceptors on the surfaces of the transducer, has proven to be crucial for biosensors effectiveness [ 4 ]. Antibody-based biosensors, i.e., immunosensors, benefit from the natural high specificity of antigen-antibody interactions.…”

Section: Introductionmentioning

confidence: 99%

Core-Shell Magnetic Nanoparticles for Highly Sensitive Magnetoelastic Immunosensor

et al. 2020

View full text Add to dashboard Cite

A magnetoelastic (ME) biosensor for wireless detection of analytes in liquid is described. The ME biosensor was tested against human IgG in the range 0–20 μg∙mL−1. The sensing elements, anti-human IgG produced in goat, were immobilized on the surface of the sensor by using a recently introduced photochemical immobilization technique (PIT), whereas a new amplification protocol exploiting gold coated magnetic nanoparticles (core-shell nanoparticles) is demonstrated to significantly enhance the sensitivity. The gold nanoflowers grown on the magnetic core allowed us to tether anti-human IgG to the nanoparticles to exploit the sandwich detection scheme. The experimental results show that the 6 mm × 1 mm × 30 μm ME biosensor with an amplification protocol that uses magnetic nanoparticles has a limit of detection (LOD) lower than 1 nM, works well in water, and has a rapid response time of few minutes. Therefore, the ME biosensor is very promising for real-time wireless detection of pathogens in liquids and for real life diagnostic purpose.

show abstract

Section: Introductionmentioning

confidence: 99%

Core-Shell Magnetic Nanoparticles for Highly Sensitive Magnetoelastic Immunosensor

et al. 2020

View full text Add to dashboard Cite

show abstract

“…One of the main issues in the development of an immunosensor platform is the way in which the recognition element and the surface of the transducer are configured, as both will greatly affect the performance of the final device in terms of its sensitivity and selectivity [8]. Α considerable amount of scientific effort has been put towards increasing the sensitivity of an immunosensor through the optimization of either the way the recognition elements is immobilized onto the transducer surface or the morphology of the surface itself or both.…”

Section: Introductionmentioning

confidence: 99%

Comparative Assessment of Affinity-Based Techniques for Oriented Antibody Immobilization towards Immunosensor Performance Optimization

et al. 2019

View full text Add to dashboard Cite

Immunosensor sensitivity and stability depend on a number of parameters such as the orientation, the surface density, and the antigen-binding efficiency of antibodies following their immobilization onto functionalized surfaces. A number of techniques have been developed to improve the performance of an immunosensor that targets one or both of the parameters mentioned above. Herein, two widely employed techniques are compared for the first time, which do not require any complex engineering of neither the antibodies nor the surfaces onto which the former get immobilized. To optimize the different surface functionalization protocols and compare their efficiency, a model antibody-antigen system was employed that resembles the complex matrices immunosensors are frequently faced with in real conditions. The obtained results reveal that protein A/G is much more efficient in increasing antibody loading onto the surfaces in comparison to boronate ester chemistry. Despite the fact, therefore, that both contribute towards the orientation-specific immobilization of antibodies and hence enhance their antigen-binding efficiency, it is the increased antibody surface density attained with the use of protein A/G that plays a critical role in achieving maximal antigen recognition.

show abstract

“…Byeon et al [31] detected S. aureus in spinach leaves with magnetoelastic biosensor operating in the range 1.0 × 10 1 -1.0 × 10 8 CFU/25 mm 2 surface of spinach. Similarly, Menti et al [32] used a magnetoelastic immunosensor in the range 10 4 -10 8 CFU/mL to detect S. aureus in a culture. In a study, S. typhimurium was detected on the tomato surface using a magnetoelastic biosensor with a working range of 5 × 10 1 -5 × 10 8 CFU/mL [35].…”

Section: Mass-sensitive Biosensorsmentioning

confidence: 99%

Biosensors: A Novel Approach to Detect Food-borne Pathogens.

Sentürk¹,

Aktop²,

Şanlibaba³

et al. 2018

Appli Microbiol Open Access

View full text Add to dashboard Cite

Foodborne pathogens affect human health negatively and are known to cause economic losses. Therefore, quick detection of foodborne pathogens and the implementation of measures to ensure their inactivation are of immense significance. Immunological, molecular, and cultural methods are frequently used in the detection of foodborne pathogens. High cost, prolonged analysis times, and the necessity of specialized personnel are some of the disadvantages of these methods. Biosensors are known as analytical devices. The use of biosensors is considered a new approach to quickly detect foodborne pathogens and their toxins. Biosensors, which are capable of converting biological, chemical, or biochemical signals into measurable electrical signals, are systems containing a biological detection material combined with a chemical or physical transducer. Different types of biosensor are being employed for detection of pathogenic bacteria. Biosensors are sensitive, fast, economical, reliable, and portable devices, and are used in many fields such as food safety, medicine, pharmacy, measurement of environmental pollution, and the military defense. Electrochemical and optical biosensors and piezoelectric immunosensors are among the most frequently used biosensors in the detection of foodborne pathogens. In this article, the principle components and requirements for an ideal biosensor, types, and their applications in food industry are summarized.

show abstract

Influence of antibody immobilization strategies on the analytical performance of a magneto-elastic immunosensor for Staphylococcus aureus detection

Cited by 13 publications

References 38 publications

Core-Shell Magnetic Nanoparticles for Highly Sensitive Magnetoelastic Immunosensor

Core-Shell Magnetic Nanoparticles for Highly Sensitive Magnetoelastic Immunosensor

Comparative Assessment of Affinity-Based Techniques for Oriented Antibody Immobilization towards Immunosensor Performance Optimization

Biosensors: A Novel Approach to Detect Food-borne Pathogens.

Contact Info

Product

Resources

About