Influence of annealing temperature on microstructures and resistivity of Fe x Al 1-x films

Identification of Dihydrobenzofuran Neolignans as Novel PDE4 Inhibitors and Evaluation of Antiatopic Dermatitis Efficacy in DNCB-Induced Mice Model

“…The expression and purification of the catalytic domains of PDE4D (GenBank: NM_001197221.1, T86-S413), PDE5A (GenBank: NM_001083.3, E535-Q860), PDE7A (GenBank: NM_001242318.2, S130−S482), PDE9A (GenBank: NM_001001567.1, P181−K506), and PDE10A (GenBank: NM_006661.3, S439-A766) were performed by the protocols reported previously. 31,36 Briefly, cDNA fragments encoding the catalytic domain of the above PDEs were separately transformed into the pET15b expression vector and were expressed in Escherichia coli BL21(DE3). The recombinant proteins were further purified by the Ni NTA column (GE Healthcare), Q-Sepharose (GE Healthcare), and superdex200 (GE Healthcare) for the enzymatic inhibition assay and protein crystallization.…”

International Perspectives

“…Other inborn errors of metabolism, such as maple syrup urine disease and galactosemia, are uncommon and rarely reported in southern Chinese. (31) The overall incidence of inborn errors of metabolism and other inherited disorders in southern Chinese is low, which might be related to strict prohibition of consanguineous marriage in the Chinese culture. (27) Glutaric aciduria type 1 (GA-1), the most frequently reported disorder, is caused by an enzymatic defect of glutaryl-CoA dehydrogenase that affects the metabolism of hydroxylysine, lysine, and tryptophan.…”