Influence of androgen deprivation therapy on choline PET/CT in recurrent prostate cancer

Dost, Rutger; Glaudemans, Andor W J M; Breeuwsma, A.J.; Jong, Igle J. de

doi:10.1007/s00259-013-2398-7

Cited by 20 publications

(12 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consequently, the preferable imaging quality and clear visualisation resulted in questions about therapy response especially in patients with metastatic PCa during androgen deprivation therapy (ADT). During the last decades, choline analogues labelled with 11 C and 18 F were the ‘gold-standard’ in nuclear medicine PCa imaging [ 18 , 19 ]. Furthermore, they were used for staging and therapy monitoring.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Alterations in androgen deprivation enhanced prostate-specific membrane antigen (PSMA) expression in prostate cancer cells as a target for diagnostics and therapy

et al. 2015

View full text Add to dashboard Cite

BackgroundProstate-specific membrane antigen (PSMA) is a promising target for diagnostics and therapy of prostate carcinoma (PCa). Based on the hypothesis that PSMA expression can be modulated by variations in androgen deprivation therapy (ADT), we investigated the binding of a PSMA-directed radiopharmaceutical in vitro in order to get an insight of the interactions between altered premedication and PSMA expression before repetitive PSMA-directed PET/CT for therapy response and targeted therapy implementation.MethodsThe human castration-resistant PCa cell line VCaP (CRPC) was treated with either 1 nmol/L testosterone (T) over 20 passages yielding the androgen-sensitive cell line (revCRPC) or with 5 μmol/L abiraterone acetate (AA) generating the abiraterone-tolerant subtype CRPCAA. In these cell lines, T and AA were varied by either supply or withdrawal of T and AA. PSMA expression of the three cell culture models was detected by Western blot and immunohistochemical staining. For quantitative measurement of tracer uptake, 0.3 nmol/L 68Ga-labelled PSMA-HBED-CC peptide (100–300 kBq/ml) was added to different treated parallel cultures (n = 9 each). Time-dependent uptake per 106 cells of each culture was calculated and evaluated. PSMA mRNA expression was investigated by qPCR.ResultsPSMA expression increased dependently on intensified ADT in all three basic cell lines. 68Ga-PSMA-HBED-CC uptake almost doubled during 3 h in all cell lines (p < 0.01). Compared to the basic cells, pre-incubation with abiraterone for 48 h resulted in a significant increased uptake in CRPC (p < 0.001). In revCRPC, 48-h AA pre-incubation resulted in an eightfold higher uptake after 3 h (p < 0.001). Additional withdrawal of external testosterone increased the uptake up to tenfold (p < 0.01). The increase of PSMA expression upon ADT and AA treatments was confirmed by qPCR and Western blot data. Furthermore, in CRPCAA, 48-h AA withdrawal increased the uptake up to fivefold (p < 0.01).ConclusionsThe investigated three PCa cell culture subtypes represent a serial preclinical model of androgen deprivation therapy as a proxy for clinical situations with differing basal PSMA expression. The uptake of PSMA-binding tracers could be stimulated by therapeutic effective short-term variation in premedication in all stages of ADT response. These complex interactions have to be considered in the interpretation of diagnostic imaging using PSMA ligands as well as in the optimal timing of PSMA-based therapies.Electronic supplementary materialThe online version of this article (doi:10.1186/s13550-015-0145-8) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Furthermore, they were used for staging and therapy monitoring. Nevertheless, 2 years ago, Dost et al reported data about the influence of ADT on choline PET/CT and commented on the lack of in vitro data on that issue [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Alterations in androgen deprivation enhanced prostate-specific membrane antigen (PSMA) expression in prostate cancer cells as a target for diagnostics and therapy

et al. 2015

View full text Add to dashboard Cite

show abstract

“…There are limited studies which have evaluated this effect. It is recommended that androgen deprivation therapy should be put on hold prior to choline PET-CT to avoid false negative outcome (27). This is of utmost importance in clinical scenario of biochemical relapse of prostate cancer in hormone naïve patients.…”

Section: -Post Hormonesmentioning

confidence: 99%

Phenotypic appearances of prostate utilizing PET-MRI and PET-CT with 68Ga-PSMA, radiolabelled choline and 68Ga-DOTATATE

Haroon

Afaq

Nuthakki

et al. 2018

Nuclear Medicine Communications

View full text Add to dashboard Cite

The objective of this study was to highlight the role of multimodality imaging and present the differential diagnosis of abnormal tracer accumulation in the prostate and periprostatic tissue. Our departments have performed molecular imaging of the prostate utilizing PET-CT and PET-MRI with a range of biomarkers including F-FDG, radiolabelled choline, Ga-DOTATATE PET-CT and Ga-PSMA images. We retrospectively reviewed the varying appearances of the prostate gland in different diseases and incidental findings in periprostatic region. The differential diagnosis of pathologies related to prostate and periprostatic tissue on multimodality imaging includes malakoplakia, rhabdomyosarcoma, lymphoma, prostate cancer, neuroendocrine tumours, uchida changes, rectoprostatic fistula, synchronous malignancies, lymphocoele and schwanoma. There exists a wide differential for abnormal tracer accumulation in the prostate gland. As a radiologist and nuclear medicine physician, it is important to be aware of range of prostatic and periprostatic pathologies.

show abstract

“…The mechanisms of choline uptake and the role of the androgen receptor in prostate cancer treatment are most relevant when considering choline PET/CT under androgen deprivation. Dost et al stated that the current working hypothesis suggesting that androgen deprivation therapy does not influence choline PET/CT has not been overruled [4]. However, one should keep in mind that related clinical studies are limited in number and were not primarily designed to prospectively assess the effect of androgen deprivation on choline uptake of prostate cancer.…”

Section: Choline Petmentioning

confidence: 99%

Prostate cancer imaging

Picchio

Piert

2013

Eur J Nucl Med Mol Imaging

View full text Add to dashboard Cite

Influence of androgen deprivation therapy on choline PET/CT in recurrent prostate cancer

Abstract: We conclude that in order to prevent false-negative choline PET/CT scans androgen deprivation should be withheld prior to scanning, especially in hormone-naïve patients.

Cited by 20 publications

References 40 publications

Alterations in androgen deprivation enhanced prostate-specific membrane antigen (PSMA) expression in prostate cancer cells as a target for diagnostics and therapy

Alterations in androgen deprivation enhanced prostate-specific membrane antigen (PSMA) expression in prostate cancer cells as a target for diagnostics and therapy

Phenotypic appearances of prostate utilizing PET-MRI and PET-CT with 68Ga-PSMA, radiolabelled choline and 68Ga-DOTATATE

Prostate cancer imaging

Contact Info

Product

Resources

About